Artificial intelligence-assisted automatic and index-based microbial single-cell sorting system for One-Cell-One-Tube

查看全文 作　　者：Zhidian [1,2,3,4]Diao;Lingyan [1,3,4]Kan;Yilong [1,3,4]Zhao;Jingyun [1,3,4]Song;Chen [1,3,4]Wang;Yang [1,3,4]Liu;Fengli [1,3,4]Zhang;Teng [1,2,3,4]Xu;Rongze [1,2,3,4]Chen;Xixian [1,2,3,4]Wang;Xiaoyan [1,2,3,4]Jing;Jian [1,2,3,4]Xu;Yuandong [1,2,3,4]Li;Bo [1,2,3,4]Ma

机构地区：[1]CAS Key Laboratory of Biofuels,Shandong Key Laboratory of Energy Genetics,Single-Cell Center,Qingdao Institute of Bioenergy and Bioprocess Technology,Chinese Academy of Sciences,Qingdao,China;[2]University of Chinese Academy of Sciences,Beijing,China;[3]Shandong Energy Institute,Qingdao,China;[4]Qingdao New Energy Shandong Laboratory,Qingdao,China.5Qingdao Single-Cell Biotechnology Co.Ltd.,Qingdao,China.

出　　处：《mLife》索引2022年第1卷第4期,共12页

基　　金：the National Key R&D Program of China(Grant No.2021YFC2101100). 摘　　要：Identification,sorting,and sequencing of individual cells directly from in situ samples have great potential for in-depth analysis of the structure and function of microbiomes.In this work,based on an artificial intelligence(AI)-assisted object detection model for cell phenotype screening and a cross-interface contact method for single-cell exporting,we developed an automatic and index-based system called EasySort AUTO,where individual microbial cells are sorted and then packaged in a microdroplet and automatically exported in a precisely indexed,“One-Cell-One-Tube”manner.The target cell is automatically identified based on an AI-assisted object detection model and then mobilized via an optical tweezer for sorting.Then,a crossinterface contact microfluidic printing method that we developed enables the automated transfer of cells from the chip to the tube,which leads to coupling with subsequent single-cell culture or sequencing.The efficiency of the system for single-cell printing is>93%.The throughput of the system for single-cell printing is~120 cells/h.Moreover,>80%of single cells of both yeast and Escherichia coli are culturable,suggesting the superior preservation of cell viability during sorting.Finally,AI-assisted object detection supports automated sorting of target cells with high accuracy from mixed yeast samples,which was validated by downstream single-cell proliferation assays.The automation,index maintenance,and vitality preservation of EasySort AUTO suggest its excellent application potential for single-cell sorting. 关键词：artificial intelligence One-Cell-One-Tube(OCOT) single-cell analysis single-cell printing single-cell sorting

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Artificial intelligence-assisted automatic and index-based microbial single-cell sorting system for One-Cell-One-Tube

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