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CRISPR/Cas9-mediated knockout of period reveals its function in the circadian rhythms of the diamondback moth Plutella xylostella

查看全文 作　　者：Danfeng [1,2,3,4,5]Wang;Jing [1,2,3,4,5]Chen;Yao [6]Yuan;Lingqi [6]Yu;Guang [1,2,3,4,5]Yang;Wenfeng [6]Chen 高影响力作者 机构地区：[1]State Key Laboratory of Ecological Pest Control for Fujian and Taiwan Crops,Institute of Applied Ecology,Fujan Agriculture and Forestry University,Fuzhou,China;[2]Joint International Research Laboratory of Ecological Pest Control,Ministry of Education,Fuzhou,China;[3]Key Laboratory of Integrated Pest Management for Fujian-Taiwan Crops,Ministry of Agriculture,Fuzhou,China;[4]Key Laboratory of Green Control of Insect Pests(Fujian Agriculture and Forestry University),Fujian Province University,Fuzhou,China;[5]Ministerial and Provincial Joint Innovation Centre for Safety Production of Cross-Strait Crops,Fujian Agriculture and Forestry University,Fuzhou,China;[6]Institute of Life Sciences,College of Biological Science and Engineering,Fuzhou University,Fuzhou,China高影响力机构 出　　处：《Insect Science》索引2023年第30卷第3期,共13页高影响力期刊 基　　金：Wenfeng Chen's lab is supported by the Qi-Shan scholar grant of Fuzhou University(GXRC-20070);the National Natural Science Foundation of China(31970461);the Natural Science Foundation of Fujian Province(2020J02027);Guang Yang's work is supported by the National Natural Science Foundation of China(grant number 31772237). 摘　　要：Circadian clocks control the rhythmicity of many behaviors and physiological features of insects.To study the circadian clock of the moth Plutella xylostella,we employed CRISPR/Cas9-mediated genome editing to investigate the effect of loss of the clock gene period on the circadian rhythms.P.xylostella harbors a single copy of period.Phylogenetic analysis showed that P.xylostella PERIOD is more homologous to mouse PERIOD than the PERIOD proteins from bees,flies,mosquitos,and many other Lepidoptera,such as Danaus plexippus and Bombyx mori.The circadian rhythms in adult locomotor activity were altered in the period knockout strain of P.xylostella under light–dark(LD)and continuous dark(DD)conditions.Under the LD cycle,the wild-type moths displayed nocturnal activity with activity peaking very early after lights off and quickly declining after lights on.In contrast,the period knockout strain had no peak in activity when the lights were turned off and exhibited steady activity throughout the hours of darkness.Interestingly,under DD conditions,our results showed that the locomotor rhythm can be maintained without period gene,but at a lower rhythmicity ratio than wild-type.In addition,knockout of period in P.xylostella changed circadian rhythms patterns related to pupal eclosion,mating,egg-laying,and egg hatching.Mechanistically,loss of PERIOD disrupted the molecular rhythm of period and changed the clock transcription rhythm in the heads of the moths under LD and DD conditions.Together,our study indicates that the PERIOD is required for normal expression of many behavioral rhythms in P.xylostella. 关 键 词：CRISPR/Cas9 circadian rhythm PERIOD Plutella xylostella
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