Neotropical mustelids: fecal metabolome diversity and its potential for taxonomic discrimination

查看全文 作　　者：Lana Resende de [1,2]ALMEIDA;Marina Amaral [3,4]ALVES;Ana Maria Obino [1]MASTELLA;Rafael [3]GARRETT;Maria João Ramos [1,2]PEREIRA 机构地区：[1]Bird and Mammal Evolution,Systematics and Ecology Lab,Programa de Pós-Graduação em Biologia Animal,Universidade Federal do Rio Grande do Sul,Porto Alegre,Rio Grande do Sul,Brazil;[2]Programa de Pós-Graduação em Biologia Animal,Universidade Federal do Rio Grande do Sul,Porto Alegre,Rio Grande do Sul,Brazil;[3]Chemistry Institute,Metabolomics Laboratory(LabMeta–LADETEC/IQ–UFRJ),Avenida Horácio Macedo,Federal University of Rio de Janeiro,Rio de Janeiro,RJ,Brazil;[4]Walter Mors Institute of Research on Natural Products,Universidade Federal do Rio de Janeiro,Rio de Janeiro,RJ,Brazil 出　　处：《Integrative Zoology》索引2023年第18卷第3期,共12页 基　　金：Lana Resende de Almeida received CAPES(Coordination for the Improvement of Higher Education Personnel from Brazil)Ph.D.scholarships:88882.439375/2019-01;Rufford Foundation Small Grant(25871-1);Maria João Ramos Pereira was supported by a CNPq(National Council for Scientific and Technological Development)Productivity Grant(311297/2018-8)。 摘　　要：Chemical profiles of non-invasive biological material,such as feces,have great potential to study elusive animals or those with low population densities.Here,we use a metabolomic approach to evaluate Neotropical mustelids as a biological model to describe the diversity of the metabolites present in fecal samples,as well as to evaluate the potential of chemical profiles for taxonomic discrimination.We collected fecal samples from captive individuals of 5 species of mustelids occurring in Brazil and analyzed them by liquid chromatography coupled to high-resolution mass spectrometry.Over 200 compounds have been annotated;“bile acids,alcohols and derivatives”was the most expressive class in the metabolome of all the species.We successfully discriminated 3 taxonomic groups:1—tayra(Eira barbara);2—otters(Lontra longicaudis and Pteronura brasiliensis;1);and 3—grisons(Galictis vittata and Galictis cuja).Several compounds seemed to be associated with food intake and the digestive process,while others were found for thefirst time in Neotropical mustelids.We concluded that mustelids show high metabolome diversity and that species-specific identification through metabolomic profiles is possible,thus contributing to the development and implementation of additional non-invasive approaches in the study of mustelids. 关 键 词：chemical profiles ECOLOGY high-resolution mass spectrometry metabolomics MUSTELIDAE