A cell wall invertase modulates resistance to fusarium crown rot and sharp eyespot in common wheat

查看全文 作　　者：Guoguo [1]Lv;Yixiao [1]Zhang;Lin [1]Ma;Xiangning [1]Yan;Mingjie [1]Yuan;Jianhui [1]Chen;Yongzhen [1]Cheng;Xi [1]Yang;Qi [1]Qiao;Leilei [1]Zhang;Mohsin [1]Niaz;Xiaonan [1]Sun;Qijun [2]Zhang;Shaobin [2]Zhong;Feng [1]Chen

机构地区：[1]National Key Laboratory of Wheat and Maize Crop Science/CIMMYT‐China Wheat and Maize Joint Research Center/Agronomy College,Henan Agricultural University,Zhengzhou 450000,China;[2]Department of Plant Pathology,North Dakota State University,Fargo,North Dakota,USA

出　　处：《Journal of Integrative Plant Biology》索引2023年第65卷第7期,共12页

基　　金：funded by the National Key Research and Development Program (2019YFE0118300);the National Natural Science Foundation (31861143008);Henan Major Science and Technology Projects (181100110200) of China。 摘　　要：Fusarium crown rot(FCR) and sharp eyespot(SE)are serious soil-borne diseases in wheat and its relatives that have been reported to cause wheat yield losses in many areas. In this study, the expression of a cell wall invertase gene, TaCWI-B1,was identified to be associated with FCR resistance through a combination of bulk segregant RNA sequencing and genome resequencing in a recombinant inbred line population. Two biparental populations were developed to further verify TaCWI-B1 association with FCR resistance.Overexpression lines and ethyl methanesulfonate(EMS) mutants revealed TaCWI-B1 positively regulating FCR resistance. Determination of cell wall thickness and components showed that the TaCWI-B1-overexpression lines exhibited considerably increased thickness and pectin and cellulose contents. Furthermore, we found that TaCWI-B1 directly interacted with an alphagalactosidase(TaGAL). EMS mutants showed that TaGAL negatively modulated FCR resistance. The expression of TaGAL is negatively correlated with TaCWI-B1 levels, thus may reduce mannan degradation in the cell wall, consequently leading to thickening of the cell wall. Additionally, TaCWI-B1-overexpression lines and TaGAL mutants showed higher resistance to SE;however, TaCWI-B1 mutants were more susceptible to SE than controls.This study provides insights into a FCR and SE resistance gene to combat soil-borne diseases in common wheat. 关键词：cell wall thickness common wheat fusarium crown rot sharp eyespot TaCWI gene

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A cell wall invertase modulates resistance to fusarium crown rot and sharp eyespot in common wheat

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