Exosome miRNAs profiling in serum and prognostic evaluation in patients with multiple myeloma

查看全文 作　　者：Teng [1,2]Fang;Hao [1,2]Sun;Xiyue [1,2]Sun;Yi [1]He;Peixia [3]Tang;Lixin [1,2]Gong;Zhen [1,2]Yu;Lanting [1,2]Liu;Shiyi [1,2]Xie;Tingyu [1]Wang;Zhenshu [3]Xu;Shuhua [1]Yi;Gang [1]An;Yan [1]Xu;Guoqing [1]Zhu;Lugui [1,2,4]Qiu;Mu [1,2]Hao

机构地区：[1]State Key Laboratory of Experimental Hematology,National Clinical Research Center for Blood Diseases,Haihe Laboratory of Cell Ecosystem,Institute of Hematology&Blood Diseases Hospital,Chinese Academy of Medical Sciences&Peking Union Medical College,Tianjin 300020,China;[2]Tianjin Institutes of Health Science,Tianjin 301600,China;[3]Hematology Department,Fujian Medical University Union Hospital,Fujian Institute of Hematology,Fuzhou 350001,China;[4]Gobroad Healthcare Group,Beijing 100072,China

出　　处：《Blood Science》索引2023年第5卷第3期,共13页

基　　金：supported by the National Natural Science Foundation of China(82170194,81920108006,82270175);the CAMS Innovation Fund for Medical Sciences(CIFMS 2021-I2M-1-040,CIFMS 2022-I2M-022). 摘　　要：MicroRNAs(MiRNAs)carried by exosomes play pivotal roles in the crosstalk between cell components in the tumor microenvironment.Our study aimed at identifying the expression profile of exosomal miRNAs(exo-miRNAs)in the serum of multiple myeloma(MM)patients and investigating the regulation networks and their potential functions by integrated bioinformatics analysis.Exosomes in serum from 19 newly diagnosed MM patients and 9 healthy donors were isolated and the miRNA profile was investigated by small RNA sequencing.Differential expression of exo-miRNAs was calculated and target genes of miRNAs were predicted.CytoHubba was applied to identify the hub miRNAs and core target genes.The LASSO Cox regression model was used to develop the prognostic model,and the ESTIMATE immune score was calculated to investigate the correlation between the model and immune status in MM patients.The top six hub differentially expressed serum exo-miRNAs were identified.513 target genes of the six hub exo-miRNAs were confirmed to be differentially expressed in MM cells in the Zhan Myeloma microarray dataset.Functional enrichment analysis indicated that these target genes were mainly involved in mRNA splicing,cellular response to stress,and deubiquitination.13 core exo-miRNA target genes were applied to create a novel prognostic signature to provide risk stratification for MM patients,which is associated with the immune microenvironment of MM patients.Our study comprehensively investigated the exo-miRNA profiles in MM patients.A novel prognostic signature was constructed to facilitate the risk stratification of MM patients with distinct outcomes. 关键词：Bioinformatics analysis EXOSOME MicroRNA Multiple myeloma Prognosis evaluation

相关文献

参考文献（56）
引证文献（2）
耦合文献（0）

参考文献(56)

引证文献(2)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Exosome miRNAs profiling in serum and prognostic evaluation in patients with multiple myeloma

参考文献(56)

引证文献(2)

用户提示