The role of CDK1 siRNA interference in cell cycle and cell apoptosis

查看全文 作　　者：Hui [1]XIAO;Ming [1]TIAN;Junna [1]GE;Xin [1]WEI;Zhaoming [1]LI;Xiaolan [1]LI;Deding [1]TAO;Junbo [1]HU;Jianping [1]GONG 机构地区：[1]Cancer Research Institute,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan 430030,China 出　　处：《Frontiers of Medicine》索引2009年第3卷第4期,共6页 基　　金：supported by grants from the National Basic Research Program of China(No.2004CB518705);the National Natural Science Foundation of China(No.30570908). 摘　　要：In the present report,cyclin-dependent kinase1(CDK1)siRNA was transfected into cells to silence the CDK1 gene expression and study its role in the cell cycle and cell apoptosis.The siRNA targeting CDK1 gene was chemically synthesized and transfected into Hela cells by lipofectamine 2000.The expression levels of CDK1 gene and protein were examined by real-time quantitative polymerase chain reaction(PCR)and Western blot,respectively.The cell cycle was analyzed by using DNA content analysis byflow cytometry.Cell apoptosis was detected by the Annexin V/PI method.The morphological changes of transfected cells were examined under the microscopy by Wright-Giemsa stain.CDK1 gene was successfully silenced by its siRNA,and the CDK1 protein expression level was decreased significantly,especially from 48th h to 60th h after transfection.The DNA content analysis showed that transfection of CDK1 siRNA led to cells accumulating in G2/M phase.There was no significant difference in the apoptotic rate between transfected cells and the control cells after transfection of CDK1 siRNA for 48 or 60 h.More double nucleus or multinucleus cells could be seen under the microscopy among the transfected cells.The decreased CDK1 expression by siRNA silencing gave rise to cell cycle arrest in G2/M phase but did not induce apoptosis. 关 键 词：cyclin-dependent kinasel siRNA interference cell cycle APOPTOSIS