Apoptosis induced by 3,7-dinitrodibenzobromonium salts in K_(562)cells

查看全文 作　　者：Ruidong [1]MIAO;Xiaohui [1]XIA;Dongling [2]YANG;Minghua [1]LÜ;Qin [1]WANG 机构地区：[1]School of Life Sciences,Lanzhou University,Lanzhou 730000,China;[2]Institute of Biophysics,Chinese Academic of Sciences,Beijing 100101,China 出　　处：《Frontiers in Biology》索引2008年第3卷第3期,共4页 摘　　要：We evaluated the inhibitory effect of 3,7-dini-trodibenzobromonium salts(cBr)on the proliferation of human chronic myelogenous leukemia K_(562)cell by trypan blue exclusion test and MTT colorimetric assay.The degree of DNA damage in K_(562)cells treated with cBr,was detected by isotopic tracer method(^(3)H-TdR).The morphological changes of these K_(562)cells were examined by fluorescence and electron microscopy.Biochemical characteristics of K_(562)cells were detected by flow cytometry and ^(3)H-thymidine incorporation assay.Findings indicated that cBr could significantly inhibit cell proliferation and result in DNA damage of K_(562)cells.cBr is a new type of immunostimulant and can induce cell apoptosis. 关 键 词：3,7-dinitrodibenzobromonium salts(cBr) anti-tumor activity APOPTOSIS