A comparative study to evaluate the effect of limited access dressing on diabetic ulcers

查看全文 作　　者：Thittamaranahalli Muguregowda [1]Honnegowda;Pramod [1,2]Kumar;Krishnananda [3]Prabhu;Ashwini [4]Kumar;Pragna [3]Rao;E.G.Padmanabha [3]Udupa;Shobha [3]Kamath;Antony Sylvan D’[5]Souza;Krishna Kishore [6]Mahato 机构地区：[1]Department of Plastic Surgery and Burns,Kasturba Medical College,Manipal 576104,Karnataka,India;[2]Department of Plastic Surgery,King Abdulaziz Specialist Hospital,Sakaka 42421,Al-Jouf,Saudi Arabia;[3]Department of Biochemistry,Kasturba Medical College,Manipal 576104,Karnataka,India;[4]Department of Forensic Medicine,Kasturba Medical College,Manipal 576104,Karnataka,India;[5]Department of Anatomy,Kasturba Medical College,Manipal 576104,Karnataka,India;[6]Department of Biophysics,School of Life Sciences,Manipal 576104,Karnataka,India 出　　处：《Plastic and Aesthetic Research》索引2015年第2卷第1期,共6页 摘　　要：Aim:Emerging evidence favors the important role of antioxidants,matrix metalloproteinases(MMPs),and nitric oxide(NO)in the healing of diabetic wounds.There is a lack of substantial evidence regarding the effects of negative pressure on antioxidants,MMPs and NO in chronic wounds associated with diabetes.Methods:A total of 55 type 2 diabetic patients with leg ulcers were divided into 2 groups:a limited access dressing(LAD)group(n=27)and a conventional dressing group(n=28).Levels of hydroxyproline,total protein,MMP-2 and MMP-9,NO and antioxidants including reduced glutathione(GSH)and the oxidative biomarker malondialdehyde(MDA)were measured in the granulation tissue at days 0 and 10.Changes in levels between the LAD and conventional groups were determined by the Student’s t-test.Results:After 10 days of treatment,the LAD vs.conventional dressing group showed increase in the levels of hydroxyproline(mean±standard deviation=55.2±25.1 vs.29.2±1,P<0.05),total protein(12.8±6.5 vs.8.34±3.2,P<0.05),NO(1.13±0.52 vs.0.66±0.43,P<0.05),GSH(7.0±2.4 vs.6.6±2.2,P<0.05)and decrease in MMP-2(0.47±0.33 vs.0.62±0.30,P<0.05),MMP-9(0.32±0.20 vs.0.53±0.39,P<0.05)and MDA(6.8±2.3 vs.10.4±3.4,P<0.05).Conclusion:When compared to conventional dressings,LAD induces biochemical changes by significantly increasing the levels of hydroxyproline,total protein,NO and antioxidants levels,and significantly reducing MMPs(MMP-2 and MMP-9)and an oxidative biomarker in diabetic wounds.These biochemical changes are thought to favor diabetic wound healing. 关 键 词：ANTIOXIDANTS diabetic wounds HYDROXYPROLINE limited access dressing matrix metalloproteinases nitric oxide reactive oxygen species and oxidative stress biomarker total protein