Hypoxia tolerance in fish depends on catabolic preference between lipids and carbohydrates

查看全文 作　　者：Qiang [1,2]Ma;Yuan [1]Luo;Jia [3]Zhong;Samwel Mchele [4]Limbu;Ling-Yu [1]Li;Li-Qiao [1]Chen;Fang [1]Qiao;Mei-Ling [1]Zhang;Qiang [3]Lin;Zhen-Yu [1]Du 机构地区：[1]LANEH,School of Life Sciences,East China Normal University,Shanghai 200241,China;[2]Yellow Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences,Qingdao,Shandong 266071,China;[3]CAS Key Laboratory of Tropical Marine Bio-Resources and Ecology,South China Sea Institute of Oceanology,Chinese Academy of Sciences,Guangzhou,Guangdong 510301,China;[4]School of Aquatic Sciences and Fisheries Technology,University of Dar es Salaam,Dar es Salaam 60091,Tanzania 出　　处：《Zoological Research》索引2023年第44卷第5期,共13页 基　　金：supported by the National Natural Science Foundation of China (31830102,32202950)。 摘　　要：Hypoxia is a common environmental stress factor in aquatic organisms,which varies among fish species.However,the mechanisms underlying the ability of fish species to tolerate hypoxia are not well known.Here,we showed that hypoxia response in different fish species was affected by lipid catabolism and preference for lipid or carbohydrate energy sources.Activation of biochemical lipid catabolism through peroxisome proliferator-activated receptor alpha(Pparα)or increasing mitochondrial fat oxidation in tilapia decreased tolerance to acute hypoxia by increasing oxygen consumption and oxidative damage and reducing carbohydrate catabolism as an energy source.Conversely,lipid catabolism inhibition by suppressing entry of lipids into mitochondria in tilapia or individually knocking out three key genes of lipid catabolism in zebrafish increased tolerance to acute hypoxia by decreasing oxygen consumption and oxidative damage and promoting carbohydrate catabolism.However,anaerobic glycolysis suppression eliminated lipid catabolism inhibition-promoted hypoxia tolerance in adipose triglyceride lipase(atgl)mutant zebrafish.Using 14 fish species with different trophic levels and taxonomic status,the fish preferentially using lipids for energy were more intolerant to acute hypoxia than those preferentially using carbohydrates.Our study shows that hypoxia tolerance in fish depends on catabolic preference for lipids or carbohydrates,which can be modified by regulating lipid catabolism. 关 键 词：Hypoxia tolerance Catabolic preference LIPID CARBOHYDRATE Oxidative damage