DNAzyme@CuSNPs dual mimic enzyme probe-amplified chemiluminescent imaging array immunosensor for multiple chicken cytokines detection

查看全文 作　　者：Jingyi [1]Zhan;Feng [1]Shi;Juan [1]Li;Hao [2,3]Zeng;Ming [1]Chen;Xiaoya [1]Hu;Zhanjun [1]Yang 机构地区：[1]School of Chemistry and Chemical Engineering,Yangzhou University,Yangzhou 225002,China;[2]Nationai Engineering Research Center of Immunological,Department of Microbiology and Biochemical Pharmacy,College of Pharmacy and Laboratory Medicine,Third Military Medical University,Chongqing 400037,China;[3]State Key Laboratory of Trauma,Burn and Combined Injury,Third Military Medical University,Chongqing 400037,China 出　　处：《Chinese Chemical Letters》索引2023年第34卷第12期,共4页 基　　金：the financial support from the National Natural Science Foundation of China(Nos.21575125 and 21475116);the Natural Science Foundation of Jiangsu Province(Nos.BK20221370 and BK20191434);Key University Natural Science Foundation of Jiangsu-Province(No.20KJA150004);Priority Academic Program Development of Jiangsu Higher Education Institution(PAPD),Project for Science and Technology of Yangzhou(No.YZ2022074);Postgraduate Research&Practice Innovation Program of Jiangsu Province(No.KYCX22_3462). 摘　　要：The detection of cytokines plays an important role in clinical diagnosis and immune mechanism research of chicken diseases.In this work,a novel and ultrasensitive chemiluminescent(CL)imaging array immunosensor was proposed to detect multiple chicken cytokines based on DNAzyme@CuS nanoparticles(DNAzyme@CuSNPs)dual mimic enzyme signal amplification strategy.DNAzyme@CuSNPs owns excellent peroxidase property,which was modified with second antibody(Ab_(2))to prepare DNAzyme@CusNPs detection probe,and demonstrated high catalysis CL imaging signal due to synergistic catalysis.Chicken interleukin-4(ChIL-4)and chicken interferon-y(ChIFN-y)were used as model analysis samples,the DNAzyme@CusSNPs-based CL imaging immunosensor achieved simultaneous and high-throughput detection of ChIL-4 and ChIFN-y with wide linear range of 10^(-3)-10^(2) ng/mL,and the detection limits are 0.41 pg/mL and 0.36 pg/mL,respectively.The multiplex chicken cytokines CL imaging array immunosensor shows a high sensitivity,wide linear range,excellent specificity and acceptable stability.This research opens dual mimic enzyme signal-amplified strategy to develop sensitive CL imaging immunoassay for chicken diseases detection application. 关 键 词：DNAzyme@CuSNPs Dual mimic enzyme Signal amplification CHEMILUMINESCENCE Array sensor Chicken cytokines