Unveiling clinical significance and tumor immune landscape of CXCL12 in bladder cancer: Insights from multiple omics analysis

查看全文 作　　者：Zhouting [1]Tuo;Dechao [2,3]Feng;Zhiwei [1]Jiang;Liangkuan [1,4]Bi;Chao [1]Yang;Qi [1]Wang

机构地区：[1]Department of Urology,the Second Affiliated Hospital of Anhui Medical University,Hefei 230601,China;[2]Department of Urology,Institute of Urology,West China Hospital,Sichuan University,Chengdu 610041,China;[3]Department of Rehabilitation,the Affiliated Hospital of Southwest Medical University,Luzhou 646000,China;[4]Department of Urology,Peking University Shenzhen Hospital,Shenzhen 518036,China

出　　处：《Chinese Journal of Cancer Research》索引2023年第35卷第6期,共16页

基　　金：supported by the Research fund of Anhui Institute of Translational Medicine (No. 2021zhyx-C62)。 摘　　要：Objective: The interplay between chemokine C-X-C motif ligand 12(CXCL12) and its specific receptors is known to trigger various signaling pathways, contributing to tumor proliferation and metastasis. Consequently,targeting this signaling axis has emerged as a potential strategy in cancer therapy. However, the precise role of CXCL12 in clinical therapy, especially in immunotherapy for bladder cancer(BCa), remains poorly elucidated.Methods: We gathered multiple omics data from public databases to unveil the clinical relevance and tumor immune landscape associated with CXCL12 in BCa patients. Univariate and multivariate Cox regression analyses were employed to assess the independent prognostic significance of CXCL12 expression and formulate a nomogram. The expression of CXCL12 in BCa cell lines and clinical tissue samples was validated using enzymelinked immunosorbent assays(ELISA) and immunohistochemistry(IHC).Results: While transcriptional expression of CXCL12 exhibited a decrease in nearly all tumor tissues, CXCL12 methylation expression was notably increased in BCa tissues. Single-cell RNA analysis highlighted tissue stem cells and endothelial cells as the primary sources expressing CXCL12. Abnormal CXCL12 expression, based on transcriptional and methylation levels, correlated with various clinical characteristics in BCa patients. Functional analysis indicated enrichment of CXCL12 and its co-expression genes in immune regulation and cell adhesion. The immune landscape analysis unveiled a significant association between CXCL12 expression and M2 macrophages(CD163~+ cells) in BCa tissues. Notably, CXCL12 expression emerged as a potential predictor of immunotherapy response and chemotherapy drug sensitivity in BCa patients.Conclusions: Taken together, these findings suggest aberrant production of CXCL12 in BCa tissues,potentially influencing the treatment responses of affected individuals. 关键词：C-X-C motif ligand 12 bladder cancer tumor immune landscape clinical significance

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Unveiling clinical significance and tumor immune landscape of CXCL12 in bladder cancer: Insights from multiple omics analysis

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