Single-cell transcriptomic atlas of goat ovarian aging

查看全文 作　　者：Dejun [1]Xu;Shuaifei [1]Song;Fuguo [1]Wang;Yawen [1]Li;Ziyuan [1]Li;Hui [1]Yao;Yongju [1]Zhao;Zhongquan [1]Zhao

机构地区：[1]Chongqing Key Laboratory of Herbivore Science,College of Animal Science and Technology,Southwest University,Chongqing 400715,China

出　　处：《Journal of Animal Science and Biotechnology》索引2024年第15卷第1期,共16页

基　　金：supported by the National Key Research and Development Program of China(2022YFD1300202);the Technology Innovation and Application Development Special Project of Chongqing(cstc2021jscx-gksb X0008);the National Natural Science Foundation of China(32102623);the National Natural Science Foundation of Chongqing(cstc2021jcyj-msxm X0875);the Ph D Train Scientific Research Project of Chongqing(CSTB2022BSXM-JCX0002)。 摘　　要：Background The ovaries are one of the first organs that undergo degenerative changes earlier in the aging process,and ovarian aging is shown by a decrease in the number and quality of oocytes.However,little is known about the molecular mechanisms of female age-related fertility decline in different types of ovarian cells during aging,especially in goats.Therefore,the aim of this study was to reveal the mechanisms driving ovarian aging in goats at single-cell resolution.Results For the first time,we surveyed the single-cell transcriptomic landscape of over 27,000 ovarian cells from newborn,young and aging goats,and identified nine ovarian cell types with distinct gene-expression signatures.Functional enrichment analysis showed that ovarian cell types were involved in their own unique biological processes,such as Wnt beta-catenin signalling was enriched in germ cells,whereas ovarian steroidogenesis was enriched in granulosa cells(GCs).Further analysis showed that ovarian aging was linked to GCs-specific changes in the antioxidant system,oxidative phosphorylation,and apoptosis.Subsequently,we identified a series of dynamic genes,such as AMH,CRABP2,THBS1 and TIMP1,which determined the fate of GCs.Additionally,FOXO1,SOX4,and HIF1A were identified as significant regulons that instructed the differentiation of GCs in a distinct manner during ovarian aging.Conclusions This study revealed a comprehensive aging-associated transcriptomic atlas characterizing the cell typespecific mechanisms during ovarian aging at the single-cell level and offers new diagnostic biomarkers and potential therapeutic targets for age-related goat ovarian diseases. 关键词：GOAT Granulosa cells Ovarian aging Single-cell transcriptomic

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Single-cell transcriptomic atlas of goat ovarian aging

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