Identification and characterization of multipotential stem cells in immortalized normal ovarian surface epithelial cells

查看全文 作　　者：Lin [1,2]Hou;Hanqing [1,2]Hong;Wenjiao [1,2]Cao;Liutong [1,2]Wei;Lichun [1,2]Weng;Shuang [1,2]Yuan;Chengqi [1,2]Xiao;Qiuwan [1,2]Zhang;Qian [1,2]Wang;Dongmei [1,2]Lai 机构地区：[1]The International Peace Maternity and Child Health Hospital,School of Medicine,Shanghai Jiao Tong University,Shanghai 200030,China;[2]Shanghai Key Laboratory of Embryo Original Diseases,Shanghai 200030,China 出　　处：《Acta Biochimica et Biophysica Sinica》索引2024年第56卷第2期,共16页 基　　金：This work was supported by the grants from the National Natural Science Foundation of China(Nos.81971334 and 82271664);the Research Projects of Shanghai Municipal Council for Science and Technology(No.20JC1412100);Shanghai Key Laboratory of Embryo Original Diseases(No.Shelab2022ZD01);Shanghai Municipal Health Committee(No.202240345). 摘　　要：The ovarian surface epithelium(OsE)is a single layer of squamous-to-cuboidal epithelial cells that experience repetitive ovulatory rupture and subsequent repair.However,the characteristics of human immortalized ovarian surface epithelial cells(IOSE80)remain elusive.This study aims to determine whether IOSE80 cells have the characteristics of stem cell proliferation and multilineage differentiation and their application in regenerative medicine.IOsE80 cells are sequenced by high-throughput transcriptome analysis,and 5 sets of public data are used to compare the differences between IosE8o cells and bone marrow mesenchymal stem cells,pluripotent stem cells,and oocytes in transcriptome profiling.The IOsE80 cells present a cobblestone-like monolayer and express the epithelial cell marker KRT18;the stem cell markers IFITM3,ALDH1A1,and VIM;lowly express stem cell marker LGR5 and germ cell markers DDX4 and DAZL.In addition,the GO terms'regulation of stem cell proliferation','epithelial cell proliferation',etc.,are significantly enriched(P<0.05).IOSE80 cells have the potential to act as mesenchymal stem cells to differentiate into adipocytes with lipid droplets,osteoblasts,and chondroblasts in vitro.IOSE80 cells express pluripotent stem cell markers,including OCT4,SSEA4,TRA-1-60,and TRA-1-81,and they can be induced into three germ layers in vitro.IOSE80 cells also form oocyte-like cells in vitro and in vivo.In addition,IOSE80 cells exhibit robust proliferation,migration,and ovarian repair functions after in vivo transplantation.This study de-monstrates that losE8o cells have the characteristics of pluripotent/multipotent stem cells,indicating their im-portant role in tissue engineering and regenerative medicine. 关 键 词：ovarian surface epithelial cell TRANSCRIPTOME multipotential stem cell OOCYTE repair function