Neutral Red Retention by Earthworm Coelomocytes: A Biomarker of Cadmium Contamination in Soil

查看全文 作　　者：S. K. GUPTA Cell Biology Section, Industrial Toxicology Research Centre, M. G. Marg, P. B. No. 80, Lucknow 226 001, India 出　　处：《Biomedical and Environmental Sciences》索引2000年第13卷第2期,共5页 摘　　要：The earthworm Metaphire posthuma were used as a model to assess the toxic potential of cadmium incorporated into the soil by environmental or human activities. The retention period of neutral red in the lysosomes of the coelomocytes was used as a biomarker. The viability of harvested coelomocytes by a non_invasive extrusion protocol was 93% with no alteration by the dye during experimentation. The control cells retained dye for 119 and 121 min in normal soil and KCl, respectively, whereas a linear decline in the retention time in the treated earthworm coelomocytes was observed. This illustrated that the presence of cadmium caused damage to the lysosomes of the coelomocytes. 关 键 词：土壤污染 镉污染 指示生物 蚯蚓体腔细胞 中性红染料滞留法