Partial protection induced by phage library-selected peptides mimicking epitopes of Schistosoma japonicum

查看全文 作　　者：[1]欧阳理;易新元Department of Pathogen Biology;曾宪芳Department of Pathogen Biology;周金春Department of Pathogen Biology;王庆林Department of Pathogen Biology;Larry Mc [2]Reynolds 机构地区：[1]Department of Pathogen Biology;[2]New England Biolabs 出　　处：《Chinese Medical Journal》索引2003年第1期,共4页 基　　金：ThisworkissupportedbygrantsfromMinistryofScienceandTechnologyofChina (No 96 A2 3 0 6 0 4),WHO/TDR (IDNo 980 2 5 5 )andNewEnglandBiolabs,Inc USA 摘　　要：Objective To obtain peptide mimicking epitopes of Schistosoma japonicum (S.japonicum) through screening of a phage peptide library and to test their potential for induction of protection. Methods S.japonicum infected sera from Microtus fortis (IMFS) and normal sera from Microtus fortis (NMFS) were used respectively to screen a 12-mers random peptide library by testing the reactivity of anti-S.japonicum serum with the phagotopes. After three rounds of biopanning, the pooled phages were used to immunize mice, after which challenge infection was performed. Results Of 12 randomly picked clones, 10 clones selected using IMFS and 7 clones selected using NMFS were shown to be antigenic. Significant reduction in adult worms (22.6%) and a high reduction (68.9%) in liver eggs were achieved following immunization with phages screened with IMFS. However, no protection was elicited by those selected with NMFS. Conclusion The results show that the phagotopes are both antigenic and immunogenic, suggesting a potential use of phage displayed peptide as novel vaccines against S. japonicum. 关 键 词：噬菌体肽文库 日本血吸虫 模拟抗原决定簇 部分保护作用