High glucose enhance expression of matrix metalloproteinase—2 in smooth muscle cells

查看全文 作　　者：HAOFeng YUJin-[1]De

机构地区：[1]DepartmentofCardiology,AffiliatedRuijinHospital,ShanghaiSecondMedicalUniversity,Shanghai200025,China

出　　处：《Acta Pharmacologica Sinica》索引2003年第24卷第6期,共5页

摘　　要：AIM:To investigate the effects of high glucose on expression of matrix metalloproteinase-2(MMP-2) in rat aortic smooth muscle cells and the influence of matrix remodeling on atherogenesis in diabetic patients. METHODS: The smooth muscle cells were cultured from the thoracic aorta of Sprague-Dawley (SD) rat. MMP-2 mRNA was determined by reverse transcriptase-polymerase chain reaction(RT-PCR),MMP-2 protein was measured by Western blotting, and MMP-2 activity in conditioned medium was observed by zymography. RESULTS:In comparison with the control, there was no difference in the expression of MMP-2 when glucose concentration was 1g/L,whereas MMP-2 activity in smooth muscle cells was significantly increased by the glucose 5 g/L(P<0.01). CONCLUSION:High glucose enhanced the expression and activity of MMP-2 in smooth muscle cells, which may provide an explanation for the phenomenon that diabetes patients are prone to have atherosclerotic lesions. 关键词：葡萄糖金属蛋白酶-2 血管平滑肌细胞明胶酶糖尿病并发症动脉硬化

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High glucose enhance expression of matrix metalloproteinase—2 in smooth muscle cells

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