维普中文期刊产品整合服务

Effects of KAI1/CD82 on biological behavior of human colorectal carcinoma cell line

查看全文 作　　者：[1]LiLiu;De-[2]HuaWu;Zu-[1]GuoLi;Guang-[1]ZhiYang;Yan-[1]QingDing 高影响力作者 机构地区：[1]DepartmentofPathology,theFirstMilitaryMedicalUniversity,Guangzhou510515,GuangdongProvince,China;[2]DepartmentofRadiationOncology,NanfangHospital,theFirstMilitaryMedicalUniversity,Guangzhou510515,GuangdongProvince,China高影响力机构 出　　处：《World Journal of Gastroenterology》索引2003年第9卷第6期,共6页高影响力期刊 基　　金：the National Natural Science Foundation,No. 31070423;the Natural Science Foundation of Guangdong Province,No.990385,970335;the Natural Science Foundation of PLA of China,No.01MA128 摘　　要：AIM: To investigate the effects of KAI1/CD82 on biological behavior of colorectal carcinoma cells. METHODS: KAI1 cDNA was transfected into highly malignant colorectal carcinoma cell line, LoVo, which had low level of endogenous KAI1 expression, and established stable transfectant clones with high KAI1/CD82 expression.The cell-cell adhesion, cell aggregation, cell-matrix adhesion and cell invasion assay were performed to determine whether KAI1 transfectant could have an effect on proliferation,adhesion and tumor metastasis in comparison with the control transfectant cells. RESULTS: KAI1 expression did not alter in vitro cell proliferation. But the KAI1 transfectant cells exhibited significantly increased homotypic cell-cell adhesion and cell aggregation in comparison with the control transfectant cells (P<0.05). Furthermore, KAI1 expression significantly suppressed the cell adhesion to extracellular matrix components and in vitro cell invasion in KAI1-transfected LoVo cells. The data indicated that KAI1 expression significantly suppressed the metastatic potential of KAI1-transfected LoVo cells. CONCLUSION: Our results suggest that KAI1 might function as a negative regulator of colorectal carcinoma metastasis. 关 键 词：结肠癌 直肠癌 KAI1 肿瘤转移
相关文献

参考文献(33)

引证文献(16)

网站首页 | 关于我们 | 联系我们 | 产品服务 | 客服中心 | 广告服务 | 版权声明 | 网站联盟 | 友情链接 | 售卡网点

版权所有© 渝B2-20050021-1　渝公网安备 50019002500403号　违法和不良信息举报中心

互联网出版许可证 新出网证(渝)字10号 全国400电话 - 免长途话费