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The culture and establishment of embryonic germ(EG)cell lines from Chinese mini swine

查看全文 作  者:[1]HSlAOCHIENTSUNG;[1]ZHONGWEIDU;[1]RONGRUI;[1]XIULANLI;[1]LINPINGBAO;[1]JUNWU;[2]SHIMINBAO;[1]ZHENYAO 高影响力作者 机构地区:[1]LaboratoryofEmbryonicStemCell,InstituteofBiochemistryandCellBiology,ShanghaiInstitutesforBiologcalSciences,ChineseAcademyofSciences,Shanqhai200031.China;[2]ShanghaiLaboratoryAnimalCenter,ChineseAcademyofScienceslShanghai200233,China高影响力机构 出  处:《Cell Research》索引2003年第13卷第3期,共8页高影响力期刊 基  金:an introductory part of a project supported by National Science Foundation of China(No.39993430). 摘  要:As a part of a basic research project on Xeno-transplantion, we have been engaged in the derivation ofembryonic stem cell lines from Chinese mini swine. Here, we reported for the first time the establishmentof two porcine EG cell lines (BPEG1 and BPEG2) from primordial germ cells of genital ridges of a 28 anda 27 d embryos respectively. Their pluripotent nature has been identified by colony morphology, markercharacterization as well as by in vitro and in vivo differentiation. These porcine EG cells are potentiallyuseful for further basic studies. 关 键 词:EG细胞 中国小猪 胚胎 体外试验
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