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Free radical scavenger effect of rebamipide in sperm processing and cryopreservation

查看全文 作　　者：Nam Cheol [1]Park;Hyun Jun [1]Park;Kyeong Mi [1]Lee;Dong Gil [1]Shin 高影响力作者 机构地区：[1]Department of Urology, College of Medicine, Pusan National University, Busan 602 739, Korea,Department of Urology, College of Medicine, Pusan National University, Busan 602 739, Korea,Department of Urology, College of Medicine, Pusan National University, Busan 602 739, Korea,Department of Urology, College of Medicine, Pusan National University, Busan 602 739, Korea高影响力机构 出　　处：《Asian Journal of Andrology》索引2003年第5卷第3期,共7页高影响力期刊 摘　　要：Alm:To study the effect of rebamipide added to semen samples and cryoprotectant on reactive oxygen species (ROS) production. Methods: Semen samples from 30 fertile and healthy volunteers were collected bymasturbation after 2 days-3 days of abstinence. After liquefaction, the specimens were diluted with sperm washmedia to a uniform density of 20×10^6/mL. Rebamipide was added to semen samples and cryoprotectant to a final concentration of 10μmol/L, 30μmol/L, 100μmol/L or 300μmol/L. Specimens were incubated at 37℃ in a 0.5 % CO2 incubator for I h or cryopreserved at -196℃ LN2 for 3 days. The sperm motility and viability and the levels of ROS and lipid peroxidation of sperm membrance were assessed before and after incubation and cryopreservation by means of computer assisted semen analyzer, eosin-nigrosin stain, chemiluminescence and thiobarbituric acid assay,respectively. Results: The sperm motility was significantly increased after incubation with 100 gmol/L and 300gmol/L rebamipide (P<0.05). After cryopreservation, the sperm motility was significantly decreased in all concentra-tions (P<0.05), but the decrease was less with 100μmol/L and 300μmol/L rebamipide than that with otherconcentrations. The sperm viability showed no significant difference before and after incubation (P>0.05). The levels of ROS and lipid peroxidation in semen were significantly decreased in proportion to the concentrations of rebamipide both after incubation and cryopreservation (P<0.05). Conclusion: Rebamipide is an effective flee radical scavenger in semen in vitro. ( Asian J Androl 2003 Sep; 5: 195-201) 关 键 词：男性不育症 抗氧化剂 精子处理 低温贮藏
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