Effects of calmodulin on DNA-binding activity of heat shock transcription factor in vitro

查看全文 作　　者：[1]LIBing;[2]LIUHongtao;等

机构地区：[1]InstituteofAgro-Physics,PhysiologyandBiochemistry.HebeiAcademyofAgriculturalandForestrySciences,Shijiazhuang050051,China;[2]InstituteofMolecularCellBiology,HebeiNormalUniversity,Shijiaz

出　　处：《Chinese Science Bulletin》索引2003年第48卷第3期,共4页

基　　金：supported by the National Key Basic Research Special Funds of China(Grant No.G1999011700);Hebei Provincial Natural Science Foundation(Grant No.301447). 摘　　要：The DNA-binding activity of heat shocktranscription factor (HSF) was induced by heat shock (HS) of a whole cell extract. Addition of antiserum, specific toCaM, to a whole cell extract reduced bind of the HSF to the heat shock element (HSE) with maize, and the re-addition of CaM to the sample restored the activity of the HSF forbinding to HSE. In addition, DNA-binding activity of the HSF was also induced by directly adding CaM to a whole cell extract at non-HS temperature with maize. Similar resultswere obtained with wheat and tomato. Our observationprovide the first example of the involvement of CaM inregulation of the DNA-binding activity of the HSF. 关键词：热休克转录因子钙调蛋白 DNA结合小麦玉米番茄植物

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Effects of calmodulin on DNA-binding activity of heat shock transcription factor in vitro

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