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Influence of expressed TRAIL on biophysical properties of the human leukemic cell line Jurkat

查看全文 作　　者：[1]KaiCHEN;[1]DanLI;[1]YuHuiJIANG;[1]WeiJuanYAO;[2]XinJuanWANG;[2]XiaoChaoWEI;[3]JingGAO;[1]LiDeXIE;[4]ZongYiYAN;[1]ZongYaoWEN;[5]ShuCHIEN 高影响力作者 机构地区：[1]HemorheologyCenter,DepartmentofBiophysics,SchoolofBasicMedicalSciences,PekingUniversity,Beijing,100083,China.;[2]DepartmentofBiochemicalandMolecularBiology,SchoolofBasicMedicalSciences,PekingUniversity,Beijing,100083,China.;[3]DepartmentofUrology,MedicalCenter,NewYorkUniversity,USA.;[4]DepartmentofMechanicsandEngineeringScience,PekingUniversity,Beijing100871,China.;[5]DepartmentofBioengineeringandWhitakerInstituteofBiomedicalEngineering,UniversityofCalifornia,SanDiego,LaJolla,CA92093-0412,USA高影响力机构 出　　处：《Cell Research》索引2004年第14卷第2期,共8页高影响力期刊 基　　金：This work was supported by The National Natural Science Foundation of China(No.30270355,No.39930110);a Doctoral Funding(No.20010001082). 摘　　要：The cDNA fragment of human TRAIL (TNF-related apoptosis inducing ligand) was cloned into RevTet-On, a Tetregulated and high-level gene expression system. The gene expression system was constructed in a human leukemic cell line: Jurkat. By using RevTet-On TRAIL gene expression system in Jurkat as a cell model, we studied the influence of TRAIL gene on the changes of cellular apoptosis before and after the TRAIL gene expression, which was induced by adding tetracycline derivative doxycycline (Dox). The results indicated that the cellular apoptosis ratio was largely dependent on the TRAIL gene expression level. Moreover, it was found that the apoptosis-inducing TRAIL could cause significant changes in the biophysical properties of Jurkat cells. The cell surface charge density decreased, the membrane fluidity declined, the elastic coefficients K1 increased, and the proportion of o-helix in membrane protein secondary structure decreased. Thus, the apoptosis-inducing TRAIL gene caused significant changes on the biomechanic properties of Jurkat cells. 关 键 词：生物物理性质 人类白血病细胞系 表达 TRAIL 细胞凋亡 基因
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