Effects of low molecular weight heparin on platelet surface P-selectin expression and serum interleukin-8 production in rats with trinitrobenzene sulphonic acid-induced colitis

查看全文 作　　者：[1]BingXia;[1]HongHan;Ke-[1]JianZhang;[1]JinLi;Guang-[2]SongGuo;Ling-[2]LingGong;Xian-[1]ChangZeng;Jun-[3]YanLiu

机构地区：[1]DepartmentofInternalMedicine,ZhongnanHospital,WuhanUniversity,Wuhan430071,HubeiProvince,China;[2]DepartmentofPathology,ZhongnanHospital,WuhanUniversity,Wuhan430071,HubeiProvince,China;[3]DepartmentofImmunology,MedicalSchool,WuhanUniversity,Wuhan430071,HubeiProvince,China

出　　处：《World Journal of Gastroenterology》索引2004年第10卷第5期,共4页

基　　金：Supported by the Hubei Provincial Natural Science Foundation,No.2000J047 摘　　要：AIM:To observe the effects of low molecular weight heparin(LMWH) on platelet surface P-selectin expression and serum interleukin-8 production in rats with trinitrobenzene sulphonic acid (TNBS) induced colitis.METHODS:Colitis was induced in female Sprauge-Dawley rats by colonic administration of 2, 4, 6-TNBS. LMWH, a dalteparin (150U/kg, 300U/kg) was subcutaneously administrated one hour before induction of colitis and went on once a day for 6 days. Then a half dose was given for the next 7 days. Control animals received the same volume of normal saline once a day for 14 days after treated by TNBS.Animals were sacrificed at 24h, days 7 and 14 after induction of colitis. The colon was excised for the evaluation of macroscopic and histological findings and TNF-α immunohistochemical assay. Platelet surface P-selectin expression was determined by radioimmunoassay and serum IL-8 production was assayed by ELISA method.RESULTS:LMWH treatment in a dose of 300U/kg for 14 days significantly improved colonic inflammation by histological examination. Serum IL-8 production in the 300U/kg treatment group was more significantly decreased at day 14 than that at 24h (P<0.05). However, platelet surface P-selectin expression and TNF-α staining in colonic tissue were not significantly different among the three groups.CONCLUSION:LMWH has an anti-infiammatory effect on TNBS induced colitis in rats. The effect is possibly related to inhibition of proinflammatory cytokine IL-8, but not involved platelet surface P-selectin expression. 关键词：P-选择素血清学检查白细胞介素-8 低分子量肝磷脂三硝基苯硫酸大肠炎动物模型

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Effects of low molecular weight heparin on platelet surface P-selectin expression and serum interleukin-8 production in rats with trinitrobenzene sulphonic acid-induced colitis

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