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Effect of in vitro interferon-beta administration on hepatitis C virus in peripheral blood mononuclear cells as a predictive marker of clinical response to interferon treatment for chronic hepatitis C

查看全文 作　　者：Kaori [1]Mochizuki;Tatehiro [1]Kagawa;Shinji [1]Takashimizu;Kazuya [1]Kawazoe;Sei-Ichiro [1]Kojima;Naruhiko [1]Nagata;Atsushi [1]Nakano;Yasuhiro [1]Nishizaki;Koichi [1]Shiraishi;Masaru [1]Itakura;Norihito [1]Watanabe;Tetsuya [1]Mine;Shohei [1]Matsuzaki 高影响力作者 机构地区：[1]Department of Internal Medicine,Division of Gastroenterology,Tokai University School of Medicine,Bohseidai,Isehara 259-1193,Japan高影响力机构 出　　处：《World Journal of Gastroenterology》索引2004年第10卷第5期,共4页高影响力期刊 摘　　要：AIM:To test whether in vitro incubation of peripheral blood mononuclear cells (PBMC) with interferon (IFN) could efficiently decrease hepatitis C virus-RNA (HCV-RNA) amount and to analyze whether this effect was associated with clinical response to IFN.METHODS:Twenty-seven patients with histologically proven chronic hepatitis C were given intravenous administration of 6 million units (MU) IFN-β daily for 6 weeks followed by three times weekly for 20 weeks. PBMC collected before IFN therapy were incubated with IFN-β and HCV-RNA in PMBC was semi-quantitatively determined.RESULTS: Twenty-five patients completed IFN therapy.Eight patients (32%) had sustained loss of serum HCV-RNA with normal serum ALT levels after IFN therapy (complete responders).HCV-RNA in PBMC was detected in all patients,whereas it was not detected in PBMC from healthy subjects.In vitro administration of IFN-β decreased the amount of HCV-RNA in PMBC in 18 patients (72%). Eight of these patients obtained complete response. On the other hand,none of the patients whose HCV-RNA in PBMC did not decrease by IFN-β was complete responders. Multiple logistic regression analysis revealed that the decrease of HCV-RNA amount in PBMC by IFN-β was the only independent predictor for complete response (P<0.05).CONCLUSION:The effect of in vitro IFN-β on HCV in PBMC reflects clinical response and would be taken into account as a predictive marker of IFN therapy for chronic hepatitis C. 关 键 词：慢性丙型肝炎 干扰素Β 外周血 单个核细胞 免疫应答 HCV-RNA
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