ANovelEndo-1,4-p-Glucanase Gene(LlpCell) Is Exclusively Expressed in Pollen and Pollen Tubes of Lilium longiflorum

查看全文 作　　者：[1]ZHOUWei;[2]HiroyukiTAKEDA;LIUXi—[1]Zhen;[2]NaokiNAKAGAWA;[2]NaokiSAKIJRAI;[1]HUANGJian;LIYi—[1]Qin 机构地区：[1]ProteinScienceLaboratoryoftheMinistryofEducation,SchoolofLifeSciencesBeijing100084,China;;[2]FacultyofIntegratedArtsHigashi-Hiroshima739—8521,Japan 出　　处：《Acta Botanica Sinica》索引2004年第46卷第2期,共6页 摘　　要：A full—lerlgth cDNA(L/pCe//)encoding an endo-1,4-β-glucanase(EGase)was isolated from pollen tubes of Lilium longiflorum Thunb.by RT-PCR and RACE.The deduced protein,which iS predicted to be a compact globular protein,is of 490 amino acids.in cluding a putative 21 amino acid-signal peptide.LlpCell shares high level of homology(about50%)with plant secreted EGases,which bear apparently neither trans-membrane domain.nor cellulose—binding domain(CBD).LIpCell belongs to gycosyl hydrolase family 9.LlpOell transcripts were detected in pollen grains.germinating pollen and elongating pollen tubes with a similar level.whereas LlpCell transcripts were not detectable in all other lily tissues examined.The specific expression pattern suggests that LlpCell is confined to lily pollen germination and pollen tube elongation. 关 键 词：1,4-β-葡聚糖内切酶 基因 克隆 麝香百合花粉 花粉管 表达 CDNA序列