Expression of TIMP-1 and TIMP-2 in rats with hepatic fibrosis

查看全文 作　　者：Qing-[3]HeNie;Guo-[1]RongDuan;Xin-[1]DongLuo;Yu-[1]MeiXie;[1]HongLuo;Yong-[1]XingZhou;Bo-[2]RongPan

机构地区：[1]ChinesePLACentreofDiagnosisandTreatmentforInfectiousDiseases,TangduHospital,FourthMilitaryMedicalUniversity,Xi'an710038,ShaanxiProvince,China;[2]DepartmentofOncology,XijingHospital,FourthMilitaryMedicalUniversity,Xi'an710032,ShaanxiProvince,China;[3]ChinesePLACentreofDiagnosisandTreatmentforInfectiousDiseases,TangduHospital,FourthMilitaryMedicalUniversity,Xi'an710038,ShaanxiProvince,China

出　　处：《World Journal of Gastroenterology》索引2004年第10卷第1期,共5页

基　　金：Supported by the Postdoctoral Science Foundation of China,No.1999-10 摘　　要：AIM: To investigate the location and expression of TIMP-1 and TIMP-2 in the liver of normal and experimental hepatic fibrosis in rats. METHODS: The rat models of experimental immunity hepatic fibrosis (n=20) were prepared by the means of immunologic attacking with human serum albumin (HSA),and normal rats (n=10) served as control group. Both immunohistochemistry and in situ hybridization methods were respectively used to detect the TIMP-1 and TIMP-2 mRNA and related antigens in liver. The liver tissue was detected to find out the gene expression of TIMP-1 and TIMP-2 with RT-PCR. RESULTS: The TIMP-1 and TIMP-2 related antigens in livers of experimental group were expressed in myofibroblasts and fibroblasts (TIMP-1: 482±65 vs 60±20; TIMP-2:336±48 vs 50±19, P<0.001). This was the most obvious in portal area and fibrous septum. The positive signals were located in cytoplasm, not in nucleus. Such distribution and location were confirmed bysitu hybridization (TIMP-1/β-actin: 1.86±0.47 vs 0.36±0.08; TIMP-2/β-actin: 1.06±0.22 vs 0.36±0.08,P<0.001). The expression of TIMP-1 and TIMP-2 was seen in the liver of normal rats, but the expression level was very low. However, the expression of TIMP-1 and TIMP-2 in the liver of experimental group was obviously high. CONCLUSION: In the process of hepatic fibrosis, fibroblasts and myofibroblasts are the major cells that express TIMPs.The more serious the hepatic fibrosis is in the injured liver,the higher the level of TIMP-1 and TIMP-2 gene expression. 关键词：肝纤维化动物模型慢性肝炎免疫组织化学金属蛋白酶

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Expression of TIMP-1 and TIMP-2 in rats with hepatic fibrosis

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