The Inhibitory Effects of Mouse ICOS-Ig Gene-Modified Mouse Dendritic Cells on T Cells

查看全文 作　　者：[1]GuohuaWang;[2]LijuanZhu;[2]PingHu;[2]HuifenZhu;[2]PingLei;[2]WenjunLiao;[2]BingYu;[2]FeiliGong;GuanxinShen 机构地区：[1]InstituteofBiochemistryandMolecularBiology,HubeiUniversity,Wuhan430062,China;[2]DepartmentofImmunology,TongjiMedicalCollegeofHuazhongUniversityofScienceandTechnology,Wuhan430030,China 出　　处：《Cellular & Molecular Immunology》索引2004年第1卷第2期,共5页 基　　金：surpported by National Key Basic Research Program of China(No.CB510008) 摘　　要：The main approach to reduce graft rejection has been focused on the development of immunosuppressive agents at present. Although these strategies have reportedly reduced graft rejection, there has been a reciprocal increase in more severe immunosuppression and lethal infections, as well as severe side effects. Blockade of costimulatory T cell response has been proved as one of useful strategies to reduce graft rejection. Furthermore,it has been shown that infusion of dendritic cells (DCs) with a potent negative regulatory ability for T cells could prolong allograft survival. In this study mouse DCs (mDCs) were transfected with the recombinant plasmid pcDNA3.0 containing mouse inducible costimulator-Ig (mICOS-Ig) cDNA by electroporation. The transient expression of mICOS-Ig in mDC could be detected by ELISA and SDS-PAGE. Mouse ICOS-Ig fusion protein expressed in mDC and mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in mixed lymphocyte culture (MLC) in vitro. Furthermore, mICOS-Ig gene-modified mDC could inhibit lymphocyte proliferation in recipient mice. These results suggested that mICOS-Ig gene-modified mDC exerted inhibitory effects on T cells, and might be suitable for treatment or prevention of graft rejection and immunopathologicdiseases. 关 键 词：ICOS-Ig 树状细胞 T细胞 免疫抑制作用 老鼠 淋巴细胞 免疫反应