Purification of Aminopeptidase N Protein and Differences in cDNAs Encoding APN1 Between Susceptible and Resistant Helicoverpa armigera Strains to Bacillus thuringiensis Toxins

查看全文 作　　者：LIANGGe-[1]mei;WANGGui-[1]rong;[2]XUGuang;WUKong-[1]ming;GUOYu-[1]yuan

机构地区：[1]InstituteofPlantProtection,ChineseAcademyofAgriculturalSciences,Beijing100094,P.R.China;[2]DepartmentofBiology,GeorgiaSouthernUniversity,GA30460-8042,USA

出　　处：《Agricultural Sciences in China》索引2004年第3卷第6期,共12页

基　　金：Research was supported by the National Natural Science Fundation of China(30200182);973 Program of Ministry of Science and Techno1ogy,China(001CB109004). 摘　　要：The brush border membrane vesicles (BBMVs) in midgut of Helicoverpa armigera weresuccessfully separated, and most of the Aminopeptidase N (APN) activities in BBMV werepreserved. The 3-[(3-chlor-amidopropyl) dimethylammonio]-1-propane-sulphonate (CHAPS)can enhance the dissolution of BBMV, and phosphatidylinositol-specific phosopholipase C(PI-PLC) can cleave the APN from midgut membrane. The APN was primarily purified usinga Mono-Q column. The results of immunoblotting showed that the 120 and 170kDa proteinsin the BBMV could bind Cry1Ac, and 120kDa APN was a glycosylphosphalidylinositol(GPI)-anchored protein. Two Bt-resistant strains (Bt-P, Bt-M) were obtained after beingselected for more than five years in laboratory using Bt insecticides and Bt transgeniccotton incorporated into diet separately. The resistance of Bt-P and Bt-M were 1083.3and 48.7 times that of susceptible strain. The genes encoding APN1 in midgut ofsusceptible and resistant H.armigera were cloned by PCR and RACE techniques. Theinferred amino acid sequences of APN1 possessed the common character of APN family ininsects. In comparison with APN1 in susceptible strain, three nucleotide mutations wereobserved in the APN1 of Bt-M strain and resulted in two amino acid replace in theputative protein sequences, and eight nucleotide mutations were observed in Bt-P strainand resulted in five amino acid replace. 关键词：氨基肽酶纯化杆菌菌株毒素 APNl 抗性

相关文献

参考文献（11）
引证文献（8）
耦合文献（78）

参考文献(11)

引证文献(8)

耦合文献(78)

互联网出版许可证新出网证(渝)字10号全国400电话 - 免长途话费

Purification of Aminopeptidase N Protein and Differences in cDNAs Encoding APN1 Between Susceptible and Resistant Helicoverpa armigera Strains to Bacillus thuringiensis Toxins

参考文献(11)

引证文献(8)

耦合文献(78)

用户提示