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| 1 | Orthotopic transplantation model of human gastrointestinal cancer and detection of micrometastases显示文摘AIM To establish a relevant animal model ofhuman gastrointestinal cancer, which can beused for repetitive investigations, so as toimprove our understanding and management ofcarcinogenesis and cancer metastasis.METHODS Intact tissues of human colorectaland pancreatic cancers were transplanted innude mice. The biological characteristics of theoriginal and the corresponding transplantedtumors were investigated by HE staining, PASstaining and immunostaining. The metastases inthe livers and lungs of nude mice wereinvestigated by immunostaining withbiotinylated mab KL-1 and by RT-PCR using CK20specific primers.RESULTS There were totally 9 of 16 surgicalspecimens growing in nude mice subcutaneouslyand/.or orthotopically (4 of 6 colorectal and 5 of10 pancreatic cancer). Tumor cell content of thespecimens and freezing of tissue specimens areimportant factors influencing the growth oftransplanted tumor. In the group of fresh tumortissues with greater than 50% tumor cellcontent, the success rate of the transplantationwas 100% (3 cases of pancreatic cancer and 3cases of colorectal cancer). The orthotopicallytransplanted tumors resemble the original tumormorphologically and biologically, including TAAexpression such as CEA byimmunohistochemistry, and CEA level in theserum of mice. Ki-67 labeling index and theexpression of TAA especially K-ras, 17-lA andRA-96, are associated with the potential of tumorgrowth in nude mice. Micrometastases in thelungs and livers of tumor bearing mice can bedetected by immunostaining with biotinylatedmab KL-1 and CK20-specific RT-PCR.CONCLUSION An orthotopic transplantationmodel for human colon and pancreatic cancer innude mice has been set up. We have alsoestablished sensitive detection methods withCK-immunohistochemistry and CK20-RT-PCR tostudy xenotransplanted human cancer and itsmetastatic cancer cells in the liver and lung ofnude mice. This study may be helpful inunderstanding the mechanism of cancermetastasis and in developing new diagnosticmethods and therapeutic strategies formetastases including micrometastases. | Jun Hui Cui~1 Uwe Krueger~2 Doris Henne-Bruns~2 Bemd Kremer~2 Holger Kalthoff~2 ~1Department of General Surgery,First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,Zhejiang Province,China ~2Department of General Surgery,Christian-Albrechts-University,Kiel,GermanyDr.Jun Hui Cui graduated from Zhejiang Medical University in 1984,earned master degree in 1990,studied in the Surgical Department of Kiel University and worked in the Lab of Molecular Oncology of Kiel University from 1994-1997achieved M.D.from Kiel University.Germany,now associate professor of surgery,specialized in colorectal oncology.Adviser of graduated student for master degree,having 20 publications published in key Chinese or English journals. | 2001 | World Journal of Gastroenterology2001，7，3： | 19 |
| 2 | The Marginal Cost Pricing as a Regulation Mechanism in Mixed Markets显示文摘 | Beato Mas -colell 1984 | 1984 | in Marchand Pestieau and Tulkens eds 'The Performance of Public Enterprises'1984，，： | 1 |
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| 4 | A seven dav lifecvcle cladoceran toxicity test显示文摘 | Mount D I Norberg T J 1984 | 1984 | Environ Toxicol Chem1984，3，： | 1 |
| 5 | Light scattering by leaf layers with application to canopy reflectance modeling:the Sail model显示文摘 | VERHOEF W 1984 | 1984 | Remote Sensing of Environment1984，16，： | 1 |
| 6 | Equilibrium between hydroxyl radicals and thallium (Ⅱ) and the oxidation potential of hydroxyl(aq)显示文摘 | Schwarz H A Dodson R'W 1984 | 1984 | J Phys Chem1984，88，16： | 1 |
| 7 | Identification of recalcitrant seeds 显示文摘 | Chin HF Hor YL Mohd Lassim MB 1984 | 1984 | Seed Sci Technol1984，12，： | 1 |
| 8 | Coevolutionary agricultural development 显示文摘 | Norgaard R (1984) | 1984 | Economic Development and Cultural Change1984，，： | 1 |
| 9 | 显示文摘 | 全国银屑病流行调查组.? 全国1984年银屑病流行调查报告(J)中华皮肤科杂志. 1986 (05) | 1986 | 中华皮肤科杂志1986，，05： | 1 |
| 10 | The analysis on landscape ecosystem of alpine tundra in Changbai Mountain显示文摘 | Huang X C 1984 | 1984 | Acta Geographica Sinica1984，39，3： | 1 |
| 11 | Chemical Preparation of GaAs Surfaces and Their Characterization by Auger Electron and X-ray Photoemission Spectroscopies 显示文摘 | cBN and N +2 ion Irradiation of Boron and Boron Journal of Vacuum Science & Technology A 1984 Chang C C Citrin P H Schwartz B | 1977 | Journal of Vacuum Science and Technology1977，14，4： | 1 |
| 12 | Dephenolizationof industrial wastewaters catalyzed by polyphenol oxidase显示文摘 | Atlow S C Bonadonna-Aparo L Klibanov A M 1984 | 1984 | Biotechnol Bioeng1984，26，： | 1 |
| 13 | The physicochemical speciation of polycyclic aromatic hydrocarbons (PAH) in aquatic systems显示文摘 | Readman J W Mantoura R F C Rhead M M 1984 | 1984 | Fresen Z Anal Chem1984，319，： | 1 |
| 14 | An examination of the Degtjarelt method of determination of soil organic matter and proposed modification of the chromic acid titration method显示文摘 | Walkley A Black A I 1984 | 1984 | Soil Sci1984，37，： | 1 |
| 15 | C, igaspora erythropa, a new species forming arbuscular mycorrhizae 显示文摘 | Koske RE Walker C 1984 | 1984 | Mycologia1984，76，： | 1 |
| 16 | The tribes of the Cruciferae (Brassieaeeae) in the southeastern United States 显示文摘 | Al-Shehbaz IA 1984 | 1984 | J Arnold Arbor1984，65，： | 1 |
| 17 | Modification of the gender of seed plant in varying conditions显示文摘 | Lloyd DG Bawa KS 1984 | 1984 | Evolutionary Biolog1984，17，： | 1 |
| 18 | An Examination of the Stationary of Multivariate Bankruptcy Prediction Models: A Methodological Study显示文摘 | Mensah 1984 | 1984 | Journal of Accounting Research1984，，22： | 1 |
| 19 | New flavones from Bauhinia championii Benth 显示文摘 | Chien CC Yuh PC Hong YH 1984 | 1984 | Chem Pharm Bull1984，32，1： | 1 |
| 20 | International Banking:A Survey and Comments显示文摘 | Aliber Robbort Z 1984 | 1984 | Journal of Money Credit and Banking1984，16，4： | 1 |
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