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| 1 | Prolonged high-fat-diet feeding promotes non-alcoholic fatty liver disease and alters gut microbiota in mice显示文摘BACKGROUND Non-alcoholic fatty liver disease (NAFLD) has become an epidemic largely due to the worldwide increase in obesity. While lifestyle modifications and pharmacotherapies have been used to alleviate NAFLD, successful treatment options are limited. One of the main barriers to finding safe and effective drugs for long-term use in NAFLD is the fast initiation and progression of disease in the available preclinical models. Therefore, we are in need of preclinical models that (1) mimic the human manifestation of NAFLD and (2) have a longer progression time to allow for the design of superior treatments. AIM To characterize a model of prolonged high-fat diet (HFD) feeding for investigation of the long-term progression of NAFLD. METHODS In this study, we utilized prolonged HFD feeding to examine NAFLD features in C57BL/6 male mice. We fed mice with a HFD (60% fat, 20% protein, and 20% carbohydrate) for 80 wk to promote obesity (Old-HFD group, n = 18). A low-fat diet (LFD)(14% fat, 32% protein, and 54% carbohydrate) was administered for the same duration to age-matched mice (Old-LFD group, n = 15). An additional group of mice was maintained on the LFD (Young-LFD, n = 20) for a shorter duration (6 wk) to distinguish between age-dependent and age-independent effects. Liver, colon, adipose tissue, and feces were collected for histological and molecular assessments.RESULTS Prolonged HFD feeding led to obesity and insulin resistance. Histological analysis in the liver of HFD mice demonstrated steatosis, cell injury, portal and lobular inflammation and fibrosis. In addition, molecular analysis for markers of endoplasmic reticulum stress established that the liver tissue of HFD mice have increased phosphorylated Jnk and CHOP. Lastly, we evaluated the gut microbial composition of Old-LFD and Old-HFD. We observed that prolonged HFD feeding in mice increased the relative abundance of the Firmicutes phylum. At the genus level, we observed a significant increase in the abundance of Adercreutzia, Coprococcus, Dorea, and Ruminococcus and decreased relative abundance of Turicibacter and Anaeroplasma in HFD mice. CONCLUSION Overall, these data suggest that chronic HFD consumption in mice can mimic pathophysiological and some microbial events observed in NAFLD patients. | Kandy T Velázquez Reilly T Enos Jackie E Bader Alexander T Sougiannis Meredith S Carson Ioulia Chatzistamou James A Carson Prakash S Nagarkatti Mitzi Nagarkatti E Angela Murphy | 2019 | World Journal of Hepatology2019，11，8： | 6 |
| 2 | 人免疫缺陷病毒感染者辅助性T淋巴细胞因子变化及机会性感染的临床观察显示文摘目的观察人免疫缺陷病毒(human immunodeficiency virus,HIV)感染者不同时期及其机会性感染者血清辅助性 T 淋巴细胞(helper T-Lymphoeytes,Th)细胞因子水平的变化规律。方法正常对照组17例。研究组 HIV 阳性85例(A 期17例,B 期29例,C 期39例),其中机会性感染31例。用流式细胞仪检测血 CD_4^+T 利 CD_8^+T 细胞,用酶联免疫吸附技术(enzyme linked immunosorbentassay,ELISA)检测血清白介素-2(interleukin-2,IL-2)、γ-干扰素(interferon-γ,IFN-γ)、白介素-6(interleukin-6,IL-6)、白介素-10(interleukin-10,IL-10),数据采用 SPSS11.0软件进行统计。结果研究组 CD_4^+细胞(361.85±230.61)10~6/L 低于对照组(772.41±161.56)10~6/L(t=6.992,P<0.01),IL-2(61.82±63.59)pg/ml 低于对照组(11 1.25±66.14)pg/ml(t=2.907,P<0.01),研究组 CD_8^+T细胞(713.36±317.59)10~6/L 高于对照组(583.24±96.28)10~6/L(t=3.127,P<0.01)、IL-10(1362.70±869.49)pg/ml 高于对照组(818.54±276.22)pg/ml(t=4.704,P<0.01)和 IL-6(1883.14±1058.61)pg/ml 高于对照组(1208.52±745.36)pg/ml(t=2.502,P<0.05)。随着病程进展,IL-2逐渐下降,C 期(51.72±62.28)pg/ml 和 B 期(69.02±62.77)pg/ml 分别低于对照组,而 IL-6、IL-10逐渐上升,C 期的 IL-6(2040.27±1078.95)pg/ml、IL-10(1472.10±982.03)pg/ml 均高于对照组;B期的 IL-10(1347.35±780.95)pg/ml 高于对照组(818.54±276.22)pg/ml。机会性感染组 IL-6(2236.24±1052.42)pg/ml 高于无机会性感染组(1680.43±1017.05)pg/ml(t=2.395,P<0.05)。结论 HIV 感染者应动态检测血清 IL-2、IL-6、IL-10的变化,同时可考虑上调 IL-2和下调 IL-6、IL-10,调整机体 TH1/TH2细胞的平衡,以延缓疾病进展。 | 饶和平 冯磊 李丹 Bader A 吴南屏 | 2007 | 中华预防医学杂志2007，41，1： | 4 |
| 3 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 4 | Aspect2 oriented programming显示文摘 | TZILLA E FILMAN R E BADER A | 2001 | Communications of the ACM2001，44，10： | 1 |
| 5 | Ablation dynamics of periodic nanostructures for polymer-based all-optical devices 显示文摘 | Klein-Wiele J H Bader M A Bauer I | 2002 | Synthetic Metals2002，127，： | 1 |
| 6 | Coordination Chemistry and Catalysis with Hemilabile Oxygen-Phosphorus Ligands显示文摘 | Bader A Linder E | 1991 | Coord Chem Rev1991，108，： | 1 |
| 7 | Tissue engineering of vascular grafts:human cell seeding of decellularised porcine matrix显示文摘 | Teebken OE Bader A Steinhoff G | 2000 | Ear J Vasc Endovasc Surg2000，19，4： | 1 |
| 8 | Hole is a novel gene product expressed in the developing heart and brain 显示文摘 | Nesset A L Bader D M | 2002 | Mech Dev2002，117，12： | 1 |
| 9 | Diagnosis and management of adult female stress urinary incontinence : guidelines for clinical practice from the French College of Gynaecologists and Obstetricians 显示文摘 | Fritel X Fauconnier A Bader G | 2010 | Eur J Obstet Gynecol Reprod Biol2010，151，1： | 1 |
| 10 | A low-cost,high-quality new drug discovery process using patient-derived induced pluripotent stem cells显示文摘 | Giri S Bader A | 2015 | Drug Discovery Today2015，20，1： | 1 |
| 11 | Theoretical study of neutral and of acid and base-promoted hydrolysis of formamide显示文摘 | Popelier P L A Bader R F W | 1992 | J Phys Chem1992，96，： | 1 |
| 12 | Production of transgenic models in hypertension 显示文摘 | Popova E Bader M Krivokharchenko A | 2004 | Methods Mol Med2004，108，： | 1 |
| 13 | 显示文摘 | BADER A LINDNER E | 1991 | Coord Chem Rev1991，108，1： | 1 |
| 14 | In vitro activities of benzimidazoles against Echinococcus muhilocularis metacestodes显示文摘 | Jura H Bader A Frosch M | 1998 | J Antimierob Agents Chemother1998，42，5： | 1 |
| 15 | Compression and microstructure of fiber plain woven cloths in the processing of polymer composites显示文摘 | Saunders R A Lekakou C Bader M G | 1998 | Composites Part A:Applied Science and Manufacturing1998，29，4： | 1 |
| 16 | Myocardial revascularization using on-pump beating heart among patients with left ventricular dysfunction显示文摘 | Darwazah A K Bader V Isleem I | 2010 | J Cardiothorac Surg2010，5，1： | 1 |
| 17 | Intellectual property management in inter- firm R&D collaborations 显示文摘 | GASSMANN O BADER M A | 2006 | Taiwan Academy of Manage-ment Journal2006，，6： | 1 |
| 18 | Hepatic tissue culture model for study of host-parasite interactions in alveolar echino- eoecosis显示文摘 | Jura H Bader A Hartmann M | 1996 | Infect Immun1996，64，9： | 1 |
| 19 | Polymer-basedwaveguides and optical switching显示文摘 | M A Bader H M Keller G Marowsky | 1998 | Optical Materials1998，9，： | 1 |
| 20 | Rapid detection of columnaris disease in channel catfish ( lctalurua punctauus ) with a new species - specific 16S rRNA gene - based PCR primer for flavobacterium colunmare显示文摘 | Bader J A Shoemaker C A Klesius P H | 2003 | J Microbiol Meth2003，52，： | 1 |
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