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| 1 | IL28B polymorphism and cytomegalovirus predict response to treatment in Egyptian HCV type 4 patients显示文摘AIM:To test whether the status of positive cytomegalovirus(CMV) DNA detection adds to the predictive value of IL28B and to further categorize C/T allele carriers.METHODS:This study included 166 chronic hepatitis C(CHC) patients who received combined interferon and ribavirin therapy for 48 wk,84 spontaneous hepatitis C virus(HCV) resolvers who were positive for IgG anti-HCV antibody and negative for HCV RNA,and 100 healthy subjects who were negative for both HCV antibodies and RNA as controls.Genomic DNA from peripheral blood was used for IL28B rs.12979860 single nucleotide polymorphism(SNP) and CMV DNA detection.A 139 bp fragment containing IL28B SNP was amplified in all subjects by polymerase chain reaction using a specifically designed primer.Then the IL28B rs.12979860 SNP was detected by restriction fragment length polymorphism(RFLP) genotyping.The presence of CMV DNA was tested by amplification of the gB1 gene using nested polymerase chain reaction.The role of CMV and IL28B rs.12979860 SNP genotypes in determining the response rate to combined interferon therapy and clinical status of patients were statistically analyzed.RESULTS:Current data showed that 67% of patients carrying the IL28B 12979860 C/C allele had a sustained viral response(SVR) while the genotypes C/T and TT were associated with lower SVR rates,50% and 48%,respectively.SVR rates for the C/C allele were lower than other HCV genotypes and/or other populations.Genotype CC was associated with the response to interferon(P = 0.025).Genotype C/C was reduced from 48% in controls to 14% in CHC patients suggesting its protective role against progression to chronicity.The majority of spontaneously cleared subjects(86%) were C/C,confirming its protective role.The C/T allele was present in 71% of CHC patients compared with 38% of controls,so the use of IL28B SNP genotyping only in these patients may be of little value as a predictor of response.CMV reactivation occurred in 40% of CHC patients.Co-infection with CMV seriously diminished the response to interferon(IFN) therapy,with SVR rates in C/C genotypes 87.5% in CMV-negative patients and 12.5% in CMV-positive patients(P < 0.0001).SVR rates among C/T carriers were reduced to < 50% in patients with positive CMV DNA while the non-response rate doubled.These data indicate that a supplemental assay for CMV viremia adds to the prognostic value of IL28B genotyping.CONCLUSION:The results suggest that both genetic(i.e.,spontaneous) and therapeutic(IFN-based therapy) arms are complementary in the battle against HCV.CMV DNA testing may be of value to better predict the response to IFN,particularly in IL28B C/T carriers. | Mostafa K El Awady Noha G Bader El Din Ashraf Tabll Yaser El Hosary Ashraf O Abdel Aziz Hesham El Khayat Mohsen Salama Tawfeek H Abdelhafez | 2013 | World Journal of Gastroenterology2013，19，2： | 8 |
| 2 | Tumor necrosis factor-α-G308A polymorphism is associated with liver pathological changes in hepatitis C virus patients显示文摘AIM To investigate the association of tumor necrosis factor alpha(TNFα)-G308 A polymorphism with different liver pathological changes in treatment-na?ve Egyptian patients infected with hepatitis C virus(HCV) genotype 4.METHODS This study included 180 subjects,composed of 120 treatment-na?ve chronic HCV patients with different fibrosis grades(F0-F4) and 60 healthy controls. The TNFα-G308 A region was amplified by PCR and the different genotypes were detected by restriction fragment length polymorphism analysis. The TNFα protein was detected by enzyme-linked immunosorbent assay. The influence of different TNFα-G308 A genotypes on TNFα expression and liver disease progression were statistically analyzed. The OR and 95%CI were calculated to assess the relative risk confidence.RESULTS Current data showed that the TNFα-G308 A SNP frequency was significantly different between controls and HCV infected patients(P = 0.001). Both the AA genotype and A allele were significantly higher in late fibrosis patients(F2-F4,n = 60) than in early fibrosis patients(F0-F1,n = 60)(P = 0.05,0.04 respectively). Moreover,the GA or AA genotypes increased the TNFα serum level greater than the GG genotype(P = 0.002). The results showed a clear association between severe liver pathological conditions(inflammation,steatosis and fibrosis) and(GA + AA) genotypes(P = 0.035,0.03,0.04 respectively). The stepwise logistic regression analysis showed that the TNFα genotypes(GA + AA) were significantly associated with liver inflammation(OR = 3.776,95%CI: 1.399-10.194,P = 0.009),severe steatosis(OR = 4.49,95%CI: 1.441-14.0,P = 0.010) and fibrosis progression(OR = 2.84,95%CI: 1.080-7.472,P = 0.034). Also,the A allele was an independent risk factor for liver inflammation(P = 0.003),steatosis(P = 0.003) and fibrosis(P = 0.014). CONCLUSION TNFα SNP at nucleotide-308 represents an important genetic marker that can be used for the prognosis of different liver pathological changes in HCV infected | Noha G Bader El Din Sally Farouk Reem El-Shenawy Marwa K Ibrahim Reham M Dawood Mostafa M Elhady Ahmed M Salem Naglaa Zayed Ahmed Khairy Mostafa K El Awady | 2016 | World Journal of Gastroenterology2016，22，34： | 3 |
| 3 | HepG2 cells support viral replication and gene expression of hepatitis C virus genotype 4 in vitro显示文摘瞄准:与丙肝的长期的复制建立一个房间文化系统病毒(HCV ) 染色体和病毒的抗原的表示在试管内。方法:HepG2 房间线被孵化与长期的丙肝从一个病人与浆液为它的危险性测试到 HCV。房间和上层清液在文化期间在各种各样的时间点被收获。文化上层清液为它感染天真的房间的能力被测试。存在减(反感觉) 在房间的核心和 E1 抗原的 RNA 海滨,和察觉被 RT-PCR 和免疫学的技术(流动血细胞计数和西方的污点) 分别地检验。结果:细胞内部的 HCV RNA 首先在 d 上被检测 3 在感染以后然后能一致地在至少三个月的一个时期上在房间和上层清液被检测。新鲜房间能从有教养的感染的房间感染上层清液。流动 cytometric 分析证明表面和在房子里使用的细胞内部的 HCV 抗原表示使 polyclonal 成为了抗体(反核心,和 anti-E1 ) 。西方的污点分析证明在分子量的产生免疫性的肽的簇的表示在一个月内在 31 和 45 kDa 之间延长了感染的房间的旧文化而这簇在 uninfected HepG2 房间是无法发现的。结论:HepG2 房间线产生 HCV 感染而且支持它的复制在试管内不仅。HCV 结构的蛋白质的表示能在感染的 HepG2 房间被检测。这些房间也能够流病毒的粒子进接着对 uninfected 房间变得传染的培养基。 | Mostafa K El-Awady Ashraf A Tabll Yasmine S El-Abd Mahmoud M Bahgat Hussein A Shoeb Samar S Youssef Noha G Bader El Din El-Rashdy M Redwan Maha El-Demellawy Moataza H Omran Wael T El-Garf Said A Goueli | 2006 | World Journal of Gastroenterology2006，12，30： | 2 |
| 4 | Electrical and optical properties of LiNbO3 显示文摘 | BENAISSA K ASHRIT P V BADER G | 1992 | Thin Solid Films1992，214，2： | 1 |
| 5 | Prevalence and correlates of insomnia in the Swedish population aged 19-75 years 显示文摘 | Ohayon M M Bader G | 2010 | Sleep Med2010，11，10： | 1 |
| 6 | Analyzing yeast protein-protein interaction data obtained from different sources显示文摘 | Bader G D Hogue C W | 2002 | Nat Biotechnol2002，20，10： | 1 |
| 7 | Integrated processing equipment显示文摘 | Bader M E Hall R P Strasser G | 1990 | Solid State Technology1990，33，5： | 1 |
| 8 | Tissue engineering of vascular grafts:human cell seeding of decellularised porcine matrix显示文摘 | Teebken OE Bader A Steinhoff G | 2000 | Ear J Vasc Endovasc Surg2000，19，4： | 1 |
| 9 | Integrated processing equipment显示文摘 | M E Bader R P Hall G Strasser | 1990 | Solid State Technology1990，33，5： | 1 |
| 10 | Diagnosis and management of adult female stress urinary incontinence : guidelines for clinical practice from the French College of Gynaecologists and Obstetricians 显示文摘 | Fritel X Fauconnier A Bader G | 2010 | Eur J Obstet Gynecol Reprod Biol2010，151，1： | 1 |
| 11 | Compression and microstructure of fiber plain woven cloths in the processing of polymer composites显示文摘 | Saunders R A Lekakou C Bader M G | 1998 | Composites Part A:Applied Science and Manufacturing1998，29，4： | 1 |
| 12 | Polymer-basedwaveguides and optical switching显示文摘 | M A Bader H M Keller G Marowsky | 1998 | Optical Materials1998，9，： | 1 |
| 13 | Compressibility and flow permeability of two-dimensional woven reinforcements in the processing of composites显示文摘 | Lekakou C Johari K Bader M G | 1996 | Polymer Composites1996，17，5： | 1 |
| 14 | Preparation of large-area 3D ordered macroporous titania films by silica colloidal crystal templating显示文摘 | Kuai Sulan Badilescu S Bader G | 2003 | Advanced Materials2003，15，： | 1 |
| 15 | Intensified surveillance after surgery for colorectal cancer significantly improves survival 显示文摘 | Laubert T Bader F G Oevermann E | 2010 | Eur J Med Res2010，15，1： | 1 |
| 16 | Leukemia inhibitory factor modulates cardiogenesis in embryoid bodies in opposite fashions 显示文摘 | Al - Dubai H Weitzer G | 2000 | Circ Res2000，86，7： | 1 |
| 17 | Bragg grating in planar polydiacty lene waveguides and their application in integrated optics 显示文摘 | Bader M A Marowsky G | 2001 | Synthetic Metals2001，124，： | 1 |
| 18 | A mass spectrometric analysis of the water-splitting reaction显示文摘 | Bader K P Renger G Schmid G H | 1993 | Photosynthesis Research1993，38，： | 1 |
| 19 | Tensor decompositions and applications显示文摘 | KOLDA T G BADER B W | | 0，，3： | 1 |
| 20 | An automated method for finding molecular complexes in large protein interaction networks显示文摘 | BADER G HOGUE C | 2003 | BMC Bioinformatics2003，4，： | 1 |
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