| 1 | Effect of Moringa oleifera on semen quality profiles in endangered Teressa goat under tropical humid island ecosystem显示文摘Objective:To investigate the effect of Moringa oleifera leaf extract on semen quality parameters in Teressa goat buck.Methods:A total of 25 semen samples from six bucks were selected for the study.Spermatozoa of 150×10^(6) were incubated in 0,300,500 and 700μg of leaf extract as groupⅠ,Ⅱ,Ⅲ and Ⅳ,respectively.Liquid stored semen samples were analysed for motility,viability,total sperm abnormalities,plasma membrane,acrosomal and nuclear integrities,seminal plasma intracellular enzymes[aspartate aminotransferase(AST),alanine aminotransferase(ALT)and lactate dehydrogenase(LDH)]and total antioxidant capacity(TAC)and spermatozoa malondialdehyde(MDA)and cholesterol efflux in comparison with the control group(groupⅠ)for up to 72 h.Results:Moringa oleifera leaf extract(500μg/150×10^(6) spermatozoa)-treated semen had significantly higher motility,viability,plasma membrane,acrosomal and nuclear integrities,and TAC(P<0.05),and had significantly lower total sperm abnormalities,AST,ALT,LDH,MDA production and cholesterol efflux compared to those in other Moringa oleifera leaf extract treated(300 or 700μg/150×10^(6) spermatozoa)and control groups at different hours of liquid storage(P<0.05).Semen quality parameters and TAC showed an increasing trend and total sperm abnormalities,MDA production,leakage of intra-cellular enzymes and cholesterol efflux showed a decreasing trend from group Ⅰto group Ⅲand then an opposite trend from group Ⅲ to group Ⅳ at different hours of liquid storage.Conclusions:Moringa oleifera leaf extract 500μg/150×10^(6) spermatozoa can be used for liquid semen preservation in Teressa goat at Andaman and Nicobar Islands. | P.Perumal Jai Sunder A.K.De A.K.Nahak D.K.Chaurasia T.C.Balamurugan D.Bhattacharya | 2024 | Asian pacific Journal of Reproduction2024，13，2： | 0 |
| 2 | Seasonal stress on semen quality profiles, seminal biochemical and oxidative stress attributes in endangered Teressa goat of Andaman and Nicobar Islands显示文摘Objective:To measure seasonal effects on semen quality profiles,seminal biochemical and oxidative stress attributes in fresh and liquid stored semen in monsoon and dry seasons.Methods:A total of 10 Teressa bucks(3-4 years)were selected from breeding farm,ICAR-Central Island Agricultural Research Institute,Port Blair,Andaman and Nicobar Islands,India.Semen samples(n=25 per season)were collected through artificial vagina method and preserved at refrigerated temperature(5℃)for 48 h using Tris citrate glucose based extender.We detected semen quality parameters[volume,mass activity,pH,sperm concentration,total motility,viability,total sperm abnormality,and plasma membrane,acrosomal and nuclear integrities],biochemical profiles[aspartate amino transferase(AST),alanine amino transferase(ALT)and total cholesterol],and oxidative stress markers[total antioxidant capacity(TAC)and malondialdehyde(MDA)]during monsoon and dry seasons.Results:Semen quality parameters significantly differed between seasons(P<0.05)and among storage periods(P<0.05).Volume,pH,mass activity,motility,viability,acrosomal,plasma membrane and nuclear integrities,and TAC were significantly higher(P<0.05).Sperm concentration,sperm abnormalities,MDA,AST,ALT and total cholesterol were significantly lower in fresh semen of monsoon than dry season(P<0.05).Motility,viability,acrosomal,plasma membrane and nuclear integrities,and TAC were significantly decreased(P<0.05)while sperm abnormality,AST,ALT,total cholesterol and MDA were significantly increased as liquid semen storage period advanced(P<0.05).Conclusions:Monsoon season has higher beneficial effects on semen quality profiles and liquid stored semen remained usable for upto 48 h.Good quality ejaculates with higher TAC and lower MDA can be cryopreserved and will be used for artificial insemination. | P.Perumal Jai Sunder A.K.De D.Bhattacharya A.K.Nahak R.Vikram E.B.Chakurkar | 2023 | Asian pacific Journal of Reproduction2023，12，6： | 0 |
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