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| 1 | Microscopy Observations of Habitable Space in Biochar for Colonization by Fungal Hyphae From Soil显示文摘Biochar is a potential micro-environment for soil microorganisms but evidence to support this suggestion is limited. We explored imaging techniques to visualize and quantify fungal colonization of habitable spaces in a biochar made from a woody feedstock. In addition to characterization of the biochar, it was necessary to optimize preparation and observation methodologies for examining fungal colonization of the biochar. Biochar surfaces and pores were investigated using several microscopy techniques. Biochar particles were compared in soilless media and after deposition in soil. Scanning electron microscopy(SEM) observations and characterization of the biochar demonstrated structural heterogeneity within and among biochar particles. Fungal colonization in and on biochar particles was observed using light, fluorescence and electron microscopy. Fluorescent brightener RR 2200 was more effective than Calcofluor White as a hyphal stain. Biochar retrieved from soil and observed using fluorescence microscopy exhibited distinct hyphal networks on external biochar surfaces. The extent of hyphal colonization of biochar incubated in soil was much less than for biochar artificially inoculated with fungi in a soilless medium. The location of fungal hyphae was more clearly visible using SEM than with fluorescence microscopy. Observations of biochar particles colonized by hyphae from soil posed a range of difficulties including obstruction by the presence of soil particles on biochar surfaces and inside pores. Extensive hyphal colonization of the surface of the biochar in the soilless medium contrasted with limited hyphal colonization of pores within the biochar. Both visualization and quantification of hyphal colonization of surfaces and pores of biochar were restricted by two-dimensional imaging associated with uneven biochar surfaces and variable biochar pore structure. There was very little colonization of biochar from hyphae in the agricultural soil used in this study. | Noraini M Jaafar Peta L Clode Lynette K Abbott | 2014 | Journal of Integrative Agriculture2014，13，3： | 10 |
| 2 | Neoadjuvant therapy for pancreas cancer: Past lessons and future therapies显示文摘Pancreatic adenocarcinoma remains a most deadly malignancy, with an overall 5-year survival of 5%. A subset of patients will be diagnosed with potentially resectable disease, and while complete surgical resection provides the only chance at cure, data from trials of postoperative chemoradiation and/or chemotherapy demonstrate a modest survival advantage over those patients who undergo resection alone. As such, most practitioners believe that completion of multimodality therapy is the optimal treatment. However, the sequence of surgery, chemotherapy and radiation therapy is frequently debated, as patients may benefit from a neoadjuvant approach by initiating chemotherapy and/or chemoradiation prior to resection. Here we review the rationale for neoadjuvant therapy, which includes a higher rate of completion of multimodality therapy, minimizing the risk of unnecessary surgical resection for patients who develop early metastatic disease, improved surgical outcomes and the potential for longer overall survival. However, there are no prospective, randomized studies of the neoadjuvant approach compared to a surgeryfirst strategy; the established and ongoing investigations of neoadjuvant therapy for pancreatic cancer are discussed in detail. Lastly, as the future of therapeutic regimens is likely to entail patient-specific genetic and molecular analyses, and the treatment that is best applied based on those data, a review of clinically relevant biomarkers in pancreatic cancer is also presented. | Jeffrey M Sutton Daniel E Abbott | 2014 | World Journal of Gastroenterology2014，20，42： | 5 |
| 3 | Prosthetic above-knee femoropopliteal bypass grafting: Five-year results of a randomized trial显示文摘 | Richard M Green William M Abbott Teruo Matsumoto Jock R Wheeler Normand Miller Frank J Veith Sam Money H Edward Garrett | 2000 | Journal of Vascular Surgery2000，，： | 2 |
| 4 | Interleuki-1 beta stimulates human mesangial cells to synthesize and release interleukin-6 and -8显示文摘 | Abbott F Rvan JJ Ceska M | 1991 | Kidney Int1991，40，4： | 1 |
| 5 | Ikappa-Balpha and IkappaBbeta possess injury context-specific functions that uniquely influence hepatic NF-kappaB induction and inflammation显示文摘 | Fan C Li Q Zhang Y Liu X Luo M Abbott D | 2004 | J Clin Invest2004，113，： | 1 |
| 6 | An introduction to the European Hydrological System-Systeme Hydrologique Europeen, 'SHE', 2 :Structure of a physically-based, distributed modelling systerr显示文摘 | Abbott M B Bathurst J C Cunge J A | 1986 | Journal of Hydrology1986，87，12： | 1 |
| 7 | Plasma fibrinogen andcoronar y heart disease in elderly Japanese-American men 显示文摘 | Sharp D S Abbott R D Burchfied C M | 1996 | Arterioscler Thromb Vase Biol1996，16，2： | 1 |
| 8 | Insulin - like growth factor I receptor gene structure 显示文摘 | Abbott A M Bueno R Pedrini M T | 1992 | Journal of Biological Chemistry1992，267，10： | 1 |
| 9 | System 21,Jupiter,A design system for two-dimensional nearly-horizontal flows显示文摘 | Abbott M B Damsgaard A Rodenhuis G S | 1973 | Journal of Hydraulic Research1973，11，： | 1 |
| 10 | Selective dorsal rhizotomy: outcome and complications in treating spastic cerebral palsy显示文摘 | Abbott R Johann-Murphy M Shiminski-Maher T | 1993 | Neurosurgery1993，33，85： | 1 |
| 11 | Ingestion of amniotic fluid enhances opiate analgesia in rats显示文摘 | KRISTAL M B THOMPSON A C ABBOTT P | 1986 | Physiology&behavior1986，38，： | 1 |
| 12 | Emissions of polychlorinated dibenzo-p-dioxins and polychlorinated dibenzofurans from the open burning of household waste in barrels显示文摘 | Lemieux P M Lutes C C Abbott J A | 2000 | Environmental Science and Technology2000，34，3： | 1 |
| 13 | Case of Aeromonas veronii( DNA Group 10 ) bacteremia 显示文摘 | ABBOTT S L SERVE H JANDA J M | 1994 | J Clin Microbiol1994，32，12： | 1 |
| 14 | An introduction to the European Hydrologic System-System Hydrologique European,SHE显示文摘 | Abbott M B Bathurst J C Cunge J A | 1986 | J of Hydrol1986，87，： | 1 |
| 15 | The insulin receptor tyrosine kinase substrate p58/53 and the insulin receptor are components of CNS synapses显示文摘 | Abbott M Wells D Fallon J | 1999 | J Neurosci1999，19，： | 1 |
| 16 | On the numerical modeling of short waves in shallow water显示文摘 | Abbott M B Petersen H M Skovgaard O | 1978 | Journal of Hydraulic Research1978，16，： | 1 |
| 17 | Optimal stimulus and noise distributions for information Wansmission via suprathreshold stochastic resonance 显示文摘 | M D McDonnell N G Stocks D Abbott | 2007 | Physical Review E2007，75，6： | 1 |
| 18 | An Introduction to the European Hydrological System-Systemc Hydrologique European,' SHE',1:History and Philosophy of a Physically-Based,Distributed Modelling System显示文摘 | Bathurst J C Cunge J A | 1986 | Journal of Hydrology1986，87，： | 1 |
| 19 | An introduction to the European Hydrological System-System Hydrologique European,'SHE' 1:History and philosophy of a physically based Distributed Modeling System显示文摘 | ABBOTT M B BATHURST J CETAL | 1986 | Journal of Hydrology1986，，： | 1 |
| 20 | A single copy of avirus-derived transgene encoding hairpin RNA gives immunityto barley yellow dwarf virUvs显示文摘 | Wang M B Abbott D C Waterhouse P M | 2000 | Molecular Plant Pathology2000，，6： | 1 |
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