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| 1 | Daily genetic profiling indicates JAK/STAT signaling promotes early hepatic stellate cell transdifferentiation显示文摘AIM: To identify signaling pathways and genes that initiate and commit hepatic stellate cells (HSCs) to transdifferentiation. METHODS: Primary HSCs were isolated from male Sprague-Dawley rats and cultured on plastic for 0-10 d. Gene expression was assessed daily (quiescent to day 10 culture-activation) by real time polymerase chain reaction and data clustered using AMADA software. The significance of JAK/STAT signaling to HSC transdifferentiation was determined by treating cells with a JAK2 inhibitor. RESULTS: Genetic cluster analyses, based on expression of these 21 genes, showed similar expression profiles on days 1-3, days 5 and 6, and days 7-10, while freshly isolated cells (day Q) and day 4 cells were genotypically distinct from any of the other days. Additionally, gene expression clustering revealed strong upregulation of interleukin-6, JAK2 and STAT3 mRNA in the early stages of activation. Inhibition of the JAK/STAT signaling pathway impeded the morphological transdifferentiation of HSCs which correlated with decreased mRNA expression of several profibrotic genes including collagens, α-SMA, PDGFR and TGFβR. CONCLUSION: These data demonstrate unique clustered genetic profiles during the daily progression of HSC transdifferentiation and that JAK/STAT signaling may be critical in the early stages of transdifferentiation. | Ashley M Lakner Cathy C Moore Alyssa A Gulledge Laura W Schrum | 2010 | World Journal of Gastroenterology2010，16，40： | 23 |
| 2 | Impact of increased adipose tissue mass on in{lamination, insulin resistance, and dyslipidemia显示文摘 | Dario A Gutierrez Michael J Puglisi Alyssa H Hasty | 2009 | Current Diabetes Reports2009，9，1： | 1 |
| 3 | Development and initial validation of a survey forassessing safety culture within commercial flight operations显示文摘 | Alyssa Mitchell Gibbons Terry L von Thaden Douglas A Wiegmann | 2006 | The International Journal of Aviation Psychology2006，16，2： | 1 |
| 4 | Practicum:Alaska's DNA database:the statute,its problems,and proposed solutions显示文摘 | Marika R Athens Alyssa A Rower | | 0，，： | 1 |
| 5 | DNA methylation is developmentally regulated for genes essential for cardiogenesis 显示文摘 | ALYSSA A CHAMBERLAIN M S MINGYAN L | 2014 | J Am Heart As- soc2014，3，00： | 1 |
| 6 | Theaflavin-3,3'-digallate,a component of black tea:an inducer of oxidative stress and apoptosis显示文摘 | Alyssa a G Schuck Miriam B Ausubel Harriet L Zuckerbraun | 2008 | Toxicology in Vitro2008，22，3： | 1 |
| 7 | Characterization of progesterone receptor isoform expression in fetal membranes显示文摘 | Mills M D Bryan Y | 2006 | American Journal of Obstetrics and Gynecology Volume2006，195，： | 1 |
| 8 | American nonprofit law in comparative perspective 显示文摘 | ALYSSA A D | 2011 | Washington University Global Studies Law Review2011，，10： | 1 |
| 9 | Proliferating reactive astrocytes are regulated by Notch-1 in the peri-infarct area after stroke显示文摘 | Shimada IS Alyssa B Alexander A | 2011 | Stroke2011，42，： | 1 |
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