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| 1 | Pioglitazone, a specific ligand of peroxisome proliferator-activated receptor-gamma, protects pancreas against acute cerulein-induced pancreatitis显示文摘ABM: To determine the effect of pioglitazone, a specific peroxisome proliferator-activated receptor-γ(PPARγ) ligand, on the development of acute pancreatitis (AP) and on the expression of heat shock protein 70 (HSP70) in the pancreas. METHODS: AP was induced in rats by subcutaneous infusion of cerulein for 5 h. Pancreatic blood flow was measured by laser Doppler flowmetry. Plasma lipase activity, interleukin-1β (IL-1β) and IL-10 were determined, Pancreatic weight and histology were evaluated and pancreatic DNA synthesis and blood flow as well as pancreatic mRNA for IL-1β and HSP70 were assessed in rats treated with pioglitazone alone or in combination with cerulein. RESULTS: Pioglitazone administered (10-100 mg/kg i.g.) 30 min before cerulein, attenuated dose-dependently the pancreatic tissue damage in cerulein-induced pancreatitis (CIP) as demonstrated by the improvement of pancreatic histology, reduction in plasma lipase activity, plasma concentration of pro-inflammatory IL-1β and its gene expression in the pancreas and attenuation of the pancreatitis-evoked fall in pancreatic blood flow. CIP increased pancreatic HSP70 mRNA and protein expression in the pancreas and this effect was enhanced by pioglitazone treatment. CONCLUSION: Pioglitazone attenuates CIP and the beneficial effect of this pioglitazone is multifactorial probably due to its anti-inflammatory activities, to the suppression of IL-1β and to the over-expression of HSP70. PPARγ ligands could represent a new therapeutic option in the treatment of AP. | Peter C Konturek Artur Dembinski Zygmunt Warzecha Grzegorz Burnat Piotr Ceranowicz Eckhart G Hahn Marcin Dembinski Romana Tomaszewska Stanislaw J Konturek | 2005 | World Journal of Gastroenterology2005，11，40： | 7 |
| 2 | MGMT and MLH1 methylation in Helicobacter pylori-infected children and adults显示文摘AIM:To evaluate the association between Helicobacter pylori(H.pylori) infection and MLH1 and MGMT methylation and its relationship with microsatellite instability(MSI).METHODS:The methylation status of the MLH1 and MGMT promoter region was analysed by methylation specific methylation-polymerase chain reaction(MSPPCR) in gastric biopsy samples from uninfected or H.pylori-infected children(n = 50),from adults with chronic gastritis(n = 97) and from adults with gastric cancer(n = 92).MLH1 and MGMT mRNA expression were measured by real-time PCR and normalised to a constitutive gene(β actin).MSI analysis was performed by screening MSI markers at 4 loci(Bat-25,Bat-26,D17S250 and D2S123) with PCR;PCR products were analysed by single strand conformation polymorphism followed by silver staining.Statistical analyses were performed with either the χ 2 test with Yates continuity correction or Fisher's exact test,and statistical significance for expression analysis was assessed using an unpaired Student's t-test.RESULTS:Methylation was not detected in the promoter regions of MLH1 and MGMT in gastric biopsy samples from children,regardless of H.pylori infection status.The MGMT promoter was methylated in 51% of chronic gastritis adult patients and was associated with H.pylori infection(P < 0.05);this region was methylated in 66% of gastric cancer patients,and the difference in the percentage of methylated samples between these patients and those from H.pylori-infected chronic gastritis patients was statistically significant(P < 0.05).MLH1 methylation frequencies among H.pylori-infected and non-infected chronic gastritis adult patients were 13% and 7%,respectively.We observed methylation of the MLH1 promoter(39%) and increased MSI levels(68%) in samples from gastric cancer patients in comparison to samples from H.pylori-infected adult chronic gastritis patients(P < 0.001 and P < 0.01,respectively).The frequency of promoter methylation for both genes was higher in gastric cancer samples than in H.pylori-positive chronic gastritis samples(P < 0.05).The levels of MLH1 and MGMT mRNA were significantly reduced in chronic gastritis samples that were also hypermethylated(P < 0.01).CONCLUSION:In summary,MGMT and MLH1 methylation did not occur in earlier-stage H.pylori infections and thus might depend on the duration of infection. | Marisa C Alvarez Juliana C Santos Nathália Maniezzo Marcelo S Ladeira Artur LC da Silva Isabel CA Scaletsky José Pedrazzoli Jr Marcelo L Ribeiro | 2013 | World Journal of Gastroenterology2013，19，20： | 5 |
| 3 | Ischemic preconditioning inhibits development of edematous cerulein-induced pancreatitis: Involvement of cyclooxygenases and heat shock protein 70显示文摘AIM: To determine whether ischemic preconditioning (IP)affects the development of edematous cerulein-induced pancreatitis and to assess the role of cyclooxygenase-1 (COX-1), COX-2, and heat shock protein 70 (HSP 70) in this process.METHODS: In male Wistar rats, IP was performed by damping of celiac artery (twice for 5 min at 5-min intervals).Thirty minutes after IP or sham operation, acute pancreatitis was induced by cerulein. Activity of COX-1 or COX-2 was inhibited by resveratrol or rofecoxib, respectively (10 mg/kg).RESULTS: IP significantly reduced pancreatic damage in cerulein-induced pancreatitis as demonstrated by the improvement of pancreas histology, reduction in serum lipase and poly-C ribonuclease activity, and serum concentration of pro-inflammatory interleukin (IL)-1β.Also, IP attenuated the pancreatitis-evoked fall in pancreatic blood flow and pancreatic DNA synthesis.Serum level of anti-inflammatory IL-10 was not affected by IP. Cerulein-induced pancreatitis and IP increased the content of HSP 70 in the pancreas. Maximal increase in HSP 70 was observed when IP was combined with cerulein-induced pancreatitis. Inhibition of COXs, especially COX-2, reduced the protective effect of IP in edematous pancreatitis.CONCLUSION: Our results indicate that IP reduces pancreatic damage in cerulein-induced pancreatitis and this effect, at least in part, depends on the activity of COXs and pancreatic production of HSP 70. | Zygmunt Warzecha Artur Dembinski Piotr Ceranowicz Stanislaw J Konturek Marcin Dembinski Wieslaw W Pawlik Romana Tomaszewska Jerzy Stachura Beata Kusnierz-Cabala Jerzy W Naskalski Peter C Konturek | 2005 | World Journal of Gastroenterology2005，11，38： | 2 |
| 4 | Parecoxib sodium administered over several days reduces pain after gynecologic surgery via laparotomy显示文摘 | Michael C Artur J Jan Kotarski | 2007 | J Clin Anesth2007，19，6： | 1 |
| 5 | Human-Gyrovirus-Apoptin triggers mitochondrial death pathway-Nut77 is required for apoptosis triggering显示文摘 | Wiem CB Artur C Mohamed E | 2014 | Neoplasia2014，16，9： | 1 |
| 6 | Application of enzymes for textile fi- bres processing 显示文摘 | Rim A Margarida C Artur CP | 2008 | Biocatalysis and Biotransformation2008，26，5： | 1 |
| 7 | Long linear arrays with time delay in tegration and element deseleetion显示文摘 | ARTURS C P | 1997 | SHE1997，3061，： | 1 |
| 8 | Experimental demonstration of surface selection rules for SERS on flat metallic surfaces 显示文摘 | LE Ru E C MEYER S ARTUR C | 2011 | Chemical Communications2011，47，13： | 1 |
| 9 | Chromones and flavanones from Artemisia campestris subsp maritime 显示文摘 | Joao M J V Artur M S S Jose A S C | 1998 | Phytochemistry1998，49，5： | 1 |
| 10 | The crystal structure of glucose-6-phosphate isomerase from Leishmania mexicarta reveals novel active site features 显示文摘 | Artur T C Paul A M Luiz F D | 2004 | Eur J Biochem2004，271，： | 1 |
| 11 | Population dynamics of filamentous bacteria in Danish wastewater treatment plants with nutrient removal 显示文摘 | Artur T M Kragelund C Eriksen P S | 2012 | Water Res2012，46，12： | 1 |
| 12 | Immunogenicity of anti-tnf- alpha agents in autoimmune diseases 显示文摘 | EMI AIKAWA N DE CARVALHO J F ARTUR ALMEIDA SILVA C | 2010 | Clinical Re- views in Allergy & Immunology2010，38，23： | 1 |
| 13 | Bacterial cel- lulose membranes as transdermal delivery systems for di- clofenac: In vitro dissolution and permeation studies 显示文摘 | Nuno H C S S Artur F R Isabel F A | 2014 | Carbohydr Polym2014，106，： | 1 |
| 14 | Sewage sludge-to-energy approaches based on anaerobic digestion and pyrolysis: Brief overview and energy efficiency assessment 显示文摘 | Yucheng C Artur P | 2011 | Renewable and Sustainable Energy Reviews2011，16，3： | 1 |
| 15 | Wound dressings for a proteolytie- rich environment显示文摘 | ANDREIA V ARTUR C P | 2011 | Appl Microbiol Biotechnol2011，90，2： | 1 |
| 16 | Differential voltagedependent K^+ channel responses during proliferation and activation in macrophages显示文摘 | Ruben V Artur E Mireia C | 2003 | The Journal of Biological Chemistry2003，278，46： | 1 |
| 17 | Immunogenicity of Anti-TNF-alpha agents in autoimmune diseases显示文摘 | Emi Aikawa N de Carvalho JF Artur Almeida Silva C | 2010 | Clin Rev Allergy lmmunol2010，38，23： | 1 |
| 18 | ^1H and ^13C NMR spectroscopy of mono-, di-, tri- and tetrasubstituted xanthones 显示文摘 | Eduarda G R F Artur M S S Jose A S C | 1998 | Magn Reson Chem1998，36，4： | 1 |
| 19 | Human-gyrovirus-apoptin triggers mitochondrial death pathway-nur77 is required for apoptosis triggering 显示文摘 | Wiem C Artur CP Mohamed EG | 2014 | Neoplasia2014，16，9： | 1 |
| 20 | ln-situ enzymatic generation of hy- drogen peroxide for bleaching purpose 显示文摘 | Lopez C Paulo Artur Cavaco | 2008 | Articles2008，1，： | 1 |
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