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| 1 | Inhibition of 12-lipoxygenase reduces proliferation and induces apoptosis of hepatocellular carcinoma cells in vitro and in vivo显示文摘BACKGROUND:12-lipoxygenase(12-LOX) has been reported to be an important gene in cancer cell proliferation and survival,and tumor metastasis.However,its role in hepatocellular carcinoma(HCC) cells remains unknown.METHODS:Expression of 12-LOX was assessed in a diethylnitrosamine-induced rat HCC model,and in SMMC-7721,HepG2 and L-02 cells using immunohistochemical staining and reverse transcriptase-polymerase chain reaction(RT-PCR).GST-π and Ki-67 were determined in vivo by immunohistochemical staining.Apoptosis was evaluated by TUNEL assay.Cell viability and apoptosis were determined by MTT assay and flow cytometry,respectively.Apoptosis-related proteins in SMMC-7721 and HepG2 cells were detected by Western blotting.RESULTS:Immunohistochemical staining and RT-PCR showed that 12-LOX was over-expressed in rat HCC and two HCC cell lines,while the expression was inhibited by baicalein,a specific inhibitor of 12-LOX.Baicalein inhibited cell proliferation and induced apoptosis in rat HCC and both cell lines in a dose-and time-dependent manner.Our in vivo study demonstrated that baicalein also reduced neoplastic nodules.Mechanistically,baicalein reduced Bcl-2 protein expression coupled with a slight increase of the expression of Bax and activation of caspase-3.Furthermore,baicalein inhibited the activation of ERK-1/2(phosphorylated).Interestingly,the effects of baicalein were reversed by 12(S)-HETE,a metabolite of 12-LOX.CONCLUSIONS:Inhibition of 12-LOX leads to reduced numbers of HCC cells,partially caused by increased apoptosis.12-LOX may be a potential molecular target for HCC prevention and treatment. | Xi-Ming Xu,Guang-Jin Yuan,Jun-Jian Deng,Hong-Ting Guo,Miao Xiang,Fang Yang,Wei Ge and Shi-You Chen Cancer Center, Renmin Hospital of Wuhan University, Wuhan 430060, China Cancer Center, the 82nd Hospital of the Chinese PLA, Huai’an 223001, China Department of Physiology, Medical College of Wuhan University, Wuhan 430071, China Department of Physiology & Pharmacology, University of Georgia, Athens, GA 30602, USA | 2012 | Hepatobiliary & Pancreatic Diseases International2012，11，2： | 20 |
| 2 | Some results on numerical divided difference formulas显示文摘The remainder estimates of numerical divided difference formula are given for the functions of lower and higher smoothness, respectively. Then several divided difference formulas with super-convergence are derived with their remainder expressions. | Wang Xinghua,Wang Heyu & Ming-Jun Lai Department of Mathematics, Zhejiang University, Hangzhou 310028, China Computing Center, Zhejiang University, Hangzhou 310012, China Department of Mathematics, University of Georgia, Athens, GA 30602-7403, USA | 2005 | Science China Mathematics2005，48，11： | 4 |
| 3 | Occupation Measures of Singularly Perturbed Markov Chains with Absorbing States | G.Yin~(1)) Department of Mathematics,Wayne State University,Detroit,MI 48202,USAQ.Zhang~(2)) Department of Mathematics,University of Georgia,Athens,GA 30602,USAG.Badowski~(3)) Department of Mathematics,Wayne State University,Detroit,MI 48202,USA | 2000 | Acta Mathematica Sinica,English Series2000，16，1： | 3 |
| 4 | Birthweight andadult health outcomes in a biethnic population in the USA显示文摘 | Valdez R Athens MA Thompson GH | 1994 | Diabetologia1994，37，： | 1 |
| 5 | Decision-Making about PSA Testing and Prostate Biopsies: A Qualitative Study Embedded in a Primary Care Randomised Trial显示文摘 | Kerry N.L. Avery Jane M. Blazeby J. Athene Lane David E. Neal Freddie C. Hamdy Jenny L. Donovan | 2007 | European Urology2007，，6： | 1 |
| 6 | Plastic deformation mechanisms in poly(acrylonitrile-butadiene styrene) [ABS]显示文摘 | Athene M. Donald Edward J. Kramer | 1982 | Journal of Materials Science1982，，： | 1 |
| 7 | Acid-base characterization of foursulfide minerals显示文摘Acid-basecharacterizationoffoursulfidemineralsLinYuhuan(ResearchCenterforEco-EnvironmentaiSciences,ChineseAcademyofSciences,B... | Lin Yuhuan(Research Center for Eco-Environmentai Sciences, Chinese Academy of Sciences, Beijing 100085, China)George W. Bailey(Environmental Research Laboratory Office of Research and Development, U. S. Environmental Protection Agency, Athens, Georgia 306 | 1994 | Journal of Environmental Sciences1994，6，2： | 1 |
| 8 | The LQG/LTR procedure for multivariable feedback control design 显示文摘 | Stein G Athens M | 1987 | IEEE Transaction on Automatic Control1987，32，2： | 1 |
| 9 | The LQG/LTR Procedure for Multivariable Feedback Control System Design显示文摘 | STEIN G ATHENS M | 1987 | IEEE Trans On Automatic Control1987，32，2： | 1 |
| 10 | Comparison of differentiated dendritic cell infiltration of autoimmune and osteoarthritis synovial tissue显示文摘 | Pettit AR Athen MJ Zehntners | 2001 | Arthritis Rheum2001，44，： | 1 |
| 11 | Structure of acetobacter cellulose composites in the hydrated slate 显示文摘 | Owen M Astley Elisabeth Chanliaud Athene M Dnnald el al | 2001 | Biological Macromolecules2001，，29： | 1 |
| 12 | Practicum:Alaska's DNA database:the statute,its problems,and proposed solutions显示文摘 | Marika R Athens Alyssa A Rower | | 0，，： | 1 |
| 13 | The LQG/LTR procedure for multiv-ariable feedback control design显示文摘 | Athens M | 1987 | IEEE Transaction on A-utomatic Control1987，32，2： | 1 |
| 14 | Prefrontal regional correlates of self-control in male psychiatric patients: Impulsivity facets and aggression显示文摘 | David A. Gansler Athene K.W. Lee Britt C. Emerton Christopher D’Amato Rafeeque Bhadelia Matthew Jerram Carl Fulwiler | 2010 | Psychiatry Research: Neuroimaging2010，，1： | 1 |
| 15 | Birthweight and adult health outcomes in a biethnie population in the USA显示文摘 | Valdez R Athens MA Thompson GH | 1994 | Diabetologia1994，37，： | 1 |
| 16 | A flexibility matrix solution of the vibration problem of plates based on the boundary element method显示文摘 | Katsikadelis J T Kandilas C B Athens | 1990 | Acta Mechanica1990，83，： | 1 |
| 17 | The LQG/LTR procedure for multivariable feedback control design显示文摘 | Stein G Athens M | 1987 | IEEE Trans On Automatic Control1987，32，2： | 1 |
| 18 | The LQG/LTR procedure for multivariable feedback control design显示文摘 | Stein G Athens M | 1987 | IEEE Transaction on Automatic Control1987，32，2： | 1 |
| 19 | R isk management of si mvast at i n or ator vast at i n i nteract ions with CY P3A4 inhibitors显示文摘 | Molden E Skovlu nd E Bra athen P | 2008 | Drug Saf2008，31，7： | 1 |
| 20 | Aqueous sensitive reaction sites in sulfonic acid-functionalized mesoporous sil- icas 显示文摘 | Morales G Athens G Chmelka B F | 2008 | J Catal2008，254，2： | 1 |
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