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您的检索式：作者名="Alberto Aloe"
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| 1 | Intranasal nerve growth factor bypasses the blood-brain barrier and affects spinal cord neurons in spinal cord injury显示文摘The purpose of this work was to investigate whether,by intranasal administration,the nerve growth factor bypasses the blood-brain barrier and turns over the spinal cord neurons and if such therapeutic approach could be of value in the treatment of spinal cord injury.Adult Sprague-Dawley rats with intact and injured spinal cord received daily intranasal nerve growth factor administration in both nostrils for 1 day or for 3 consecutive weeks.We found an increased content of nerve growth factor and enhanced expression of nerve growth factor receptor in the spinal cord 24 hours after a single intranasal administration of nerve growth factor in healthy rats,while daily treatment for 3 weeks in a model of spinal cord injury improved the deficits in locomotor behaviour and increased spinal content of both nerve growth factor and nerve growth factor receptors.These outcomes suggest that the intranasal nerve growth factor bypasses blood-brain barrier and affects spinal cord neurons in spinal cord injury.They also suggest exploiting the possible therapeutic role of intranasally delivered nerve growth factor for the neuroprotection of damaged spinal nerve cells. | Luigi Aloe Patrizia Bianchi Alberto De Bellis Marzia Soligo Maria Luisa Rocco | 2014 | Neural Regeneration Research2014，9，10： | 17 |
| 2 | Esti- mating irrigation water requirements in Europe显示文摘 | Gunter Wriedt Marijn Van der Velde Alberto Aloe | 2009 | Journal of Hydrology2009，373，： | 1 |
| 3 | Dual crop coefficients for maize in southern Brazil: Model testing for sprinkler and drip irrigation and mulched soil显示文摘 | Juliano D. Martins Gon?alo C. Rodrigues Paula Paredes Reimar Carlesso Zanandra B. Oliveira Alberto E. Knies Mirta T. Petry Luis S. Pereira | 2013 | Biosystems Engineering2013，，3： | 1 |
| 4 | Growth inhibitory effects of Phyllanthus niruri extracts in combination with cisplatin on cancer cell lines显示文摘AIM:To investigate the cytotoxic effects of spray-dried extracts of Phyllanthus niruri in combination with cisplatin on two cancer cell lines.METHODS:Colorectal carcinoma(HT29) and human hepatocellular carcinoma(HepG2) cells were treated with spray-dried extracts of Phyllanthus niruri(SDEPN) either alone or in combination with cisplatin at different concentrations(0.5 mg/mL and 1 mg/mL) for 4 h and 24 h.To verify and quantify cancer cells treated with these products as well as identify the cell cycle stage and cell viability,we stained the cells with propidium iodide and assessed them by flow cytometry.The percentage of cells in different cell cycle phases was quantified and data were expressed as histograms.Significant differences between groups were determined using analysis of variance and Bonferroni's test,as indicated.A value of P < 0.05 was considered to be statistically significant.RESULTS:SDEPN had significantly different cytotoxic effects on HT29(2.81 ± 0.11 vs 3.51 ± 1.13,P > 0.05) and HepG2(5.07 ± 0.3 vs 15.9 ± 1.04,P < 0.001) cells when compared to control cells for 4 h.SDEPN also had significantly different cytotoxic effects on HT29(1.91 ± 0.57 vs 4.53 ± 1.22,P > 0.05) and HepG2(14.56 ± 1.6 vs 35.67 ± 3.94,P < 0.001) cells when compared to control cells for 24 h.Both cell lines were killed by cisplatin in a dose-dependent manner compared to control cells(HepG2 cells for 4 h:10.78 ± 1.58 vs 53.89 ± 1.53,P < 0.001;24 h:8.9 ± 1.43 vs 62.78 ± 1.87,P < 0.001 and HT29 cells for 4 h:9.52 ± 0.913 vs 49.86 ± 2.89,P < 0.001;24 h:11.78 ± 1.05 vs 53.34 ± 2.65,P < 0.001).In HT29 cells,pretreatment with SDEPN and subsequent treatment with cis-platin resulted in a greater number of cells being killed(12.78 ± 1.01 vs 93.76 ± 1.6,P < 0.001).HepG2 cells showed significant cell killing with treatment with SDEPN when combined with cisplatin(12.87 ± 2.78 vs 78.8 ± 3.02,P < 0.001).CONCLUSION:SDEPN is selectively toxic against two cancer cell lines.Moreover,SDEPN in combination with cisplatin induces a synergistic increase in the cell death of both HT29 and HepG2 cells. | Raimundo Fernandes de Araújo Júnior Luiz Alberto Lira Soares Cínthia Raquel da Costa Porto Ranniere Gurgel Furtado de Aquino Hugo Gon alo Guedes Pedro Ros Petrovick Tatiane Pereira de Souza Aurigena Antunes de Araújo Gerlane Coelho Bernardo Guerra | 2012 | World Journal of Gastroenterology2012，18，31： | 0 |
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