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| 1 | An atypical NLR gene confers bacterial wilt susceptibility in Arabidopsis显示文摘Quantitative disease resistance(QDR)remains the most prevalent form of plant resistance in crop fields and wild habitats.Genome-wide association studies(GWAS)have proved to be successful in deciphering the quantitative genetic basis of complex traits such as QDR.To unravel the genetics of QDR to the devastating worldwide bacterial pathogen Ralstonia solanacearum,we performed a GWAS by challenging a highly polymorphic local mapping population of Arabidopsis thaliana with four R.solanacearum type III effector(T3E)mutants,identified as key pathogenicity determinants after a first screen on an A.thaliana core collection of 25 accessions.Although most quantitative trait loci(QTLs)were highly specific to the identity of the T3E mutant(ripAC,ripAG,ripAQ,and ripU),we finely mapped a common QTL located on a cluster of nucleotide-binding domain and leucine-rich repeat(NLR)genes that exhibited structural variation.We functionally validated one of these NLRs as a susceptibility factor in response to R.solanacearum,named it Bacterial Wilt Susceptibility 1(BWS1),and cloned two alleles that conferred contrasting levels of QDR.Further characterization indicated that expression of BWS1 leads to suppression of immunity triggered by different R.solanacearum effectors.In addition,we showed a direct interaction between BWS1 and RipAC T3E,and BWS1 and SUPPRESSOR OF G2 ALLELE OF skp1(SGT1b),the latter interaction being suppressed by RipAC.Together,our results highlight a putative role for BWS1 as a quantitative susceptibility factor directly targeted by the T3E RipAC,mediating negative regulation of the SGT1-dependent immune response. | Choghag Demirjian Narjes Razavi Gang Yu Baptiste Mayjonade Lu Zhang Fabien Lonjon Fabien Chardon Se bastien Carrere Je rome Gouzy Ste phane Genin Alberto PMacho Fabrice Roux Richard Berthome Fabienne Vailleau | 2023 | Plant Communications2023，4，5： | 0 |
| 2 | Large-scale identification of expression quantitative trait loci in Arabidopsis reveals novel candidate regulators of immune responses and other processes显示文摘The extensive phenotypic diversity within natural populations of Arabidopsis is associated with differences in gene expression.Transcript levels can be considered as in-heritable quantitative traits,and used to map expression quantitative trait loci(eQTL)in genome-wide association studies(GWASs).In order to identify putative genetic de-terminants for variations in gene expression,we used pub-licly available genomic and transcript variation data from 665 Arabidopsis accessions and applied the single nucleotide polymorphism-set(Sequence)Kernel Association Test(SKAT)method for the identification of eQTL.Moreover,we used the penalized orthogonal-components regression(POCRE)method to increase the power of statistical tests.Then,gene annotations were used as test units to identify genes that are associated with natural variations in transcript accumulation,which correspond to candidate regulators,some of which may have a broad impact on gene ex-pression.Besides increasing the chances to identify real as-sociations,the analysis using POCRE and SKAT significantly reduced the computational cost required to analyze large datasets.As a proof of concept,we used this approach to identify eQTL that represent novel candidate regulators of immune responses.The versatility of this approach allows its application to any process that is subjected to natural var-iation among Arabidopsis accessions. | Xingang Wang Min Ren Danni Liu Dabao Zhang Cuijun Zhang Zhaobo Lang Alberto PMacho Min Zhang Jian-Kang Zhu | 2020 | Journal of Integrative Plant Biology2020，62，10： | 0 |
| 3 | The transcriptional regulator JAZ8 interacts with the C2 protein from geminiviruses and limits the geminiviral infection in Arabidopsis显示文摘Jasmonates(JAs) are phytohormones that finely regulate critical biological processes, including plant development and defense. JASMONATE ZIM-DOMAIN(JAZ) proteins are crucial transcriptional regulators that keep JA-responsive genes in a repressed state. In the presence of JA-Ile, JAZ repressors are ubiquitinated and targeted for degradation by the ubiquitin/proteasome system,allowing the activation of downstream transcription factors and, consequently, the induction of JA-responsive genes. A growing body of evidence has shown that JA signaling is crucial in defending against plant viruses and their insect vectors. Here, we describe the interaction of C2proteins from two tomato-infecting geminiviruses from the genus Begomovirus, tomato yellow leaf curl virus(TYLCV) and tomato yellow curl Sardinia virus(TYLCSaV), with the transcriptional repressor JAZ8 from Arabidopsis thaliana and its closest orthologue in tomato, SlJAZ9. Both JAZ and C2proteins colocalize in the nucleus, forming discrete nuclear speckles. Overexpression of JAZ8did not lead to altered responses to TYLCV infection in Arabidopsis;however, knock-down of JAZ8 favors geminiviral infection. Low levels of JAZ8 likely affect the viral infection specifically,since JAZ8-silenced plants neither display obvious developmental phenotypes nor present differences in their interaction with the viral insect vector. In summary, our results show that the geminivirus-encoded C2 interacts with JAZ8 in the nucleus, and suggest that this plant protein exerts an anti-geminiviral effect. | Tabata Rosas-Diaz Pepe Cana-Quijada Mengshi Wu Du Hui Gemma Fernandez-Barbero Alberto PMacho Roberto Solano Araceli GCastillo Xiao-Wei Wang Rosa Lozano-Duran Eduardo R.Bejarano | 2023 | Journal of Integrative Plant Biology2023，65，7： | 0 |
| 4 | A plasma membrane nucleotide-binding leucinerich repeat receptor mediates the recognition of the Ralstonia pseudosolanacearum effector RipY in Nicotiana benthamiana显示文摘Bacterial wilt disease caused by several Ralstonia species is one of the most destructive diseases in Solanaceae crops.Only a few functional resistance genes against bacterial wilt have been cloned to date.Here,we showthat the broadly conserved typeⅢsecreted effector RipY is recognized by the Nicotiana benthamiana immune system,leading to cell death induction,induction of defense-related gene expression,and restriction of bacterial pathogen growth.Using a multiplexed virus-induced gene-silencing-based N.benthamiana nucleotide-binding and leucine-rich repeat receptor(NbNLR)library,we identified a coiled-coil(CC)nucleotide-binding and leucine-rich repeat receptor(CNL)required for recognition of RipY,which we named RESISTANCE TO RALSTONIA SOLANACEARUM RIPY(RRS-Y).Genetic complementation assays in RRS-Y-silenced plants and stable rrs-y knockout mutants demonstrated that RRS-Y is sufficient to activate RipY-induced cell death andRipY-induced immunity to Ralstonia pseudosolanacearum.RRS-Y function is dependent on the phosphate-binding loop motif of the nucleotide-binding domain but independent of the characterized signaling components ENHANCED DISEASE SUSCEPTIBILITY 1,ACTIVATED DISEASE RESISTANCE 1,and N REQUIREMENT GENE 1 and the NLR helpers NB-LRR REQUIRED FOR HR-ASSOCIATED CELL DEATH-2,-3,and-4 in N.benthamiana.We further show that RRS-Y localization at the plasma membrane is mediated by two cysteine residues in the CC domain and is required for RipY recognition.RRSY also broadly recognizes RipY homologs across Ralstonia species.Lastly,we show that the C-terminal region of RipY is indispensable for RRS-Y activation.Together,our findings provide an additional effector/receptor pair system to deepen our understanding of CNL activation in plants. | Boyoung Kim Wenjia Yu Haseong Kim Qian Dong Sera Choi Maxim Prokchorchick Alberto PMacho Kee Hoon Sohn Cécile Segonzac | 2023 | Plant Communications2023，4，6： | 0 |
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