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| 1 | 糖橙新品种‘橘湘元’的选育显示文摘'橘湘元’是从埃及糖橙芽变中选育的无核糖橙新品种。果实圆球形,果形指数为0.95。平均单果质量173 g,大小较均匀,果面较光滑,有光泽,果肉橙黄色,平均每果实含0.4粒种子。可食率达77.09%,果实出汁率54.6%,可溶性固形物13.63%,可滴定酸0.14%,维生素C含量60.57 mg·100 mL^(-1)。‘橘湘元’在树形树势、叶片大小、花瓣宽度等方面与埃及糖橙均存在明显差异。果实生育期260 d,在湖南省永兴县11月中下旬成熟。SSR结果分析表明‘橘湘元’在300 bp处较‘埃及糖橙’多1条带,具有遗传特异性。‘橘湘元’可在山地和旱地种植,表现生长快,抗性较强。 | 龙桂友 黄洪 张凤 Alessandra Gentile 邓子牛 李娜 | 2019 | 果树学报2019，36，3： | 8 |
| 2 | 转基因枳橙中rolABC基因荧光定量表达分析方法的建立显示文摘以转rolABC基因枳橙B、D、E系及野生植株为材料,制备标准品,采用SYBR Green I染料,构建rol基因和β-actin基因的双标准曲线,校正引物扩增效率后,用2-△△Ct法对rol基因在嫩茎、嫩叶、功能叶、树皮和根中的mRNA水平进行相对定量,建立适合于rolA、rolB、rolC基因实时荧光定量RT-PCR分析方法.初步认为rolC基因在嫩茎、嫩叶、功能叶和树皮中表达量最高;其次是rolA基因,主要在嫩茎中表达;rolB基因表达量最低,主要在根系中表达. | 袁飞荣 严佳文 罗坤 Alessandra Gentile 王晓君 刘东波 邓子牛 | 2010 | 湖南农业大学学报(自然科学版)2010，36，6： | 4 |
| 3 | 转基因枳橙中GA20ox1与rol基因互作关系的研究显示文摘为分析转rolABC基因枳橙GA20ox1基因与rol基因表达的互作关系,进一步阐释其矮化性状形成的分子机制。以转rol基因枳橙实生苗为试验材料,研究其对赤霉素的敏感反应,用荧光定量RT-PCR分析GA20ox1基因和rol基因的表达,并检测幼芽中过氧化物酶活性和植物内源激素含量的变化。结果表明转rol基因枳橙既不属于GA缺陷型,也不属于GA不敏感型,喷施GA3能促进其茎伸长生长,但恢复不到野生型水平,幼芽中IAA(P<0.01)、GA1和GA4(P<0.05)显著降低,POD酶活性显著提高(P<0.01)。转rol基因枳橙幼芽中GA20ox1基因mRNA水平相比对照显著下调(P<0.01)。B、D系与野生型嫩茎中无明显差异,B、D系老叶中明显降低,E系中嫩茎和老叶中均明显增加。B、D和E系嫩叶中GA20ox1基因转录表达均较野生型高。在幼芽、嫩茎中,rolC基因与GA20ox1表达负相关。rol基因通过在幼芽中的高表达下调了GA20ox1基因转录表达,进而抑制了活性GAs在幼芽中的合成,顶端分生组织较低量的活性GAs限制植物茎伸长,在转rolABC基因枳橙矮化性状建成中发挥重要作用。 | 袁飞荣 李芳 蒋巧巧 Alessandra Gentile 严佳文 邓子牛 | 2011 | 中国农学通报2011，27，19： | 3 |
| 4 | HLA class II favors clearance of HCV infection and progression of the chronic liver damage显示文摘 | Alessandra Mangia Raffaela Gentile Isabella Cascavilla Maurizio Margaglione Maria Rosaria Villani Francesco Stella Giovanni Modola Valeria Agostiano Carlo Gaudiano Angelo Andriulli | 1999 | Journal of Hepatology1999，，6： | 1 |
| 5 | HLA class II favors clearance of HCV infection and progression of the chronic liver damage显示文摘 | Alessandra Mangia Raffaela Gentile Isabella Cascavilla Maurizio Margaglione Maria Rosaria Villani Francesco Stella Giovanni Modola Valeria Agostiano Carlo Gaudiano Angelo Andriulli | 1999 | Journal of Hepatology1999，，6： | 1 |
| 6 | 枳橙CPMAX2基因的克隆、载体构建及其表达研究显示文摘转rol ABC基因枳橙莲座状分枝、矮化性状突出。为探索其侧枝生长的植物激素调控机制,通过RT-PCR法从枳橙中克隆到独脚金内酯信号转导关键元件CPMAX2基因,分析了该基因在转rol ABC基因枳橙腋生嫩叶中的转录表达,构建了CPMAX2植物过表达载体。研究表明:CPMAX2与甜橙中同源基因一致性为99.66%,编码694个氨基酸,具有一个典型的F-box蛋白结构域和两个LRR重复区,与甜橙MAX2氨基酸序列一致性为99.14%。CPMAX2在转rol ABC基因枳橙3个株系腋生嫩叶中的转录表达均比野生型显著下调。试验表明CPMAX2在转rol ABC基因枳橙莲座型分枝生长中扮演重要角色。 | 袁飞荣 袁耀明 李智鑫 Alessandra Gentile 邓子牛 | 2015 | 园艺学报2015，42，12： | 1 |
| 7 | High Insulin‐Induced Down‐Regulation of Erk‐1/IGF‐1R/FGFR‐1 Signaling Is Required for Oxidative Stress‐Mediated Apoptosis of Adipose‐Derived Stem Cells显示文摘 | Maria Giovanna Scioli Valerio Cervelli Gaetano Arcuri Pietro Gentile Elena Doldo Alessandra Bielli Elena Bonanno Augusto Orlandi | 2014 | Physiol2014，，12： | 1 |
| 8 | Defence-related gene expression in transgenic lemon plants producing an antimicrobial Trichoderma harzianum endochitinase during fungal infection显示文摘 | Gaetano Distefano Stefano La Malfa Alessandro Vitale Matteo Lorito Ziniu Deng Alessandra Gentile | 2008 | Transgenic Research2008，，5： | 1 |
| 9 | Bioinformatics and Expression Analysis of CPMAX2 in Citrange显示文摘Transgenic citrange lines with rol ABC genes behave rosette branching and extreme dwarfing.To explore the regulatory mechanism of plant hormones in axillary shoot growth of transgenic citrange,a full length cD NA of CPMAX2 was cloned from citrange [Citrus sinensis(L.) Osb × Poncirus trifoliate(L.) Raf.] by RT-PCR in this study.The expression of CPMAX2 was detected in axillary tender leaves of 3 transgenic citrange lines and the wild type.Additionally,we constructed an over-expression vector CPMAX2-p CAMBIA1301 for further study.The results showed that the c DNA sequence and its putative peptide sequence shared 99.66% and 99.14% of identity with its Citrus sinensis ortholog MAX2.The deduced amino acid sequence contained putatively one F-box and two LRR repeat domains that are highly conserved in MAX2 genes.CPMAX2 was obviously down-expressed in tender leaves of 3 transgenic citrange lines compared with the wild type.The results suggest CPMAX2 play an important role in regulating the rosette shoot growth in transgenic citrange with rol ABC genes. | YUAN Feirong YUAN Yaoming LI Zhixin Alessandra GENTILE DENG Ziniu | 2016 | Horticultural Plant Journal2016，2，3： | 0 |
| 10 | 利用番茄果实特异启动子E8改造‘外源基因清除’的表达载体及转化金柑研究显示文摘利用'外源基因清除'技术(‘Gene-Deletor’),将外源基因从转基因金柑果实中彻底清除,可以达到用转基因作物生产出非转基因食品的目的。本研究利用引物pE8F和pE8R从樱桃番茄中克隆果实特异启动子E8,通过SalⅠ和SmaⅠ双酶切置换掉'外源基因清除'载体(pCambia-LF-polseed-FLP,简称Y-A)中的花粉、种子特异启动子PAB5,重新构造'外源基因清除'载体Y-A-E8,利用农杆菌EHA105介导转化实生态金柑,获得转化植株,并通过nptⅡ基因特异引物nptⅡ-F和nptⅡ-R PCR检测阳性转化植株。载体Y-A-E8 SalⅠ和SmaⅠ双酶切结果表明,E8启动子成功替换启动子PAB5,Y-A-E8经EHA105介导转化成功获得11株转化植株且PCR检测到6株为Y-A-E8转基因植株。研究结果可为'外源基因清除'技术在转基因金柑方面的应用提供参考。 | 谢玉明 李益 严佳文 姚润贤 Alessandra Gentile 邓子牛 | 2015 | 分子植物育种2015，13，10： | 0 |
| 11 | Perimesencephalic Subarachnoid Hemorrhage in Pregnancy: A Case Report显示文摘Perimesencephalic subarachnoid hemorrhage (P-SAH) is a benign subset of subarachnoid hemorrhage. Only two cases of P-SAH during pregnancy have been reported in the literature. We describe a case of P-SAH detected at 29 weeks’ gestation, arose with severe bilateral frontal headache, neck rigidity, and vomiting. A diagnosis of P-SAH was confirmed on magnetic resonance angiography with no evidence of vascular malformation or bleeding sources and conservatively treated. Hemorrhage, vasospasm, and cerebellar edema observed on initial magnetic resonance angiography resolved after two months. The clinical course of P-SAH during pregnancy appears to be favorable. Our case shows for the first time in literature that magnetic resonance angiography is a valid diagnostic test for P-SAH during pregnancy that circumvents radiological risk associated with conventional imaging. | Stefania Ruggieri Maurizia Gatto Maria Annunziata Gentile Salvatore D'Oria Filippo Tamma Domenico Caringella Alessandra Giocolano Chiara Taccalti Maurizio Guido | 2022 | Maternal-Fetal Medicine2022，4，4： | 0 |
| 12 | 转基因枳橙中GA20ox1与rol ABC基因互作关系的研究 | 袁飞荣 李芳 蒋巧巧 Alessandra Gentile 严佳文 邓子牛 | 2011 | 武汉生物工程学院学报2011，，3： | 0 |
| 13 | 枳橙CPMAX2基因的克隆、载体构建及其表达研究显示文摘转rolABC基因枳橙莲座状分枝、矮化性状突出。为探索其侧枝生长的植物激素调控机制,本研究通过RT-PCR法从枳橙中克隆到独脚金内酯信号转导关键元件CPMAX2基因,分析了该基因在转rolABC基因枳橙腋生嫩叶中的转录表达,构建了CPMAX2植物过表达载体。研究表明:CPMAX2与甜橙中同源基因一致性为99.66%,编码694个氨基酸,具有一个典型的Fbox蛋白结构域和两个LRR重复区,与甜橙MAX2氨基酸序列一致性为99.14%。CPMAX2在转rolABC基因枳橙3个株系腋生嫩叶中的转录表达均比野生型显著下调。实验表明CPMAX2在转rolABC基因枳橙莲座型分枝生长中扮演重要角色。 | 袁飞荣 袁耀明 李智鑫 Alessandra Gentile 邓子牛 | 2016 | 武汉生物工程学院学报2016，0，1： | 0 |
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