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| 1 | In vivo anti-tumor activity of murine hematopoietic stem cells expressing a p185HER2-specific chimeric T-cell receptor gene显示文摘We have confirmed efficient anti-tumor activities of the peripheral lymphocytes transduced with a p185HER2-specific chimeric T-cell receptor gene both in murine and in human in our previous studies. To further test the feasibility of chimeric T-cell receptor in a bone marrow transplantation model, we first, made two murine tumor cell lines: MT901 and MCA-205, to express human p185HER2 by retroviral gene transduction. Murine bone marrow cells were retrovirally transduced to express the chimeric T-cell receptor and gene-modified bone marrow cells were transplanted into lethally irradiated mouse. Six months post transplantation, p185HER2-positive tumor cells:MT-901/HER2 or MCA-205/ HER2 was subcutaneously or intravenously injected to make mouse models simulating primary breast cancer or pulmonary metastasis. The in vivo anti-tumor effects were monitored by the size of the subcutaneous tumor or counting the tumor nodules in the lungs after India ink staining. The size of the subcutaneous tumor was significantly inhibited and the number of pulmonary nodules were significantly decreased in mouse recipients transplanted with chimeric T-cell receptor modified bone marrow cells compared with the control group. Our results suggest the efficient in vivo anti-tumor activities of chimeric T-cell receptor gene modified bone marrow cells. | JIAN MIN YANG MICHAEL S FRIEDMAN MARIANNE T HUBEN JENNIFER FULLER QIAO LI ALFRED E CHANG JAMES J MULE KEVIN T MCDONAGH | 2006 | Journal of Microbiology and Immunology2006，4，2： | 3 |
| 2 | 转染嵌合性T细胞受体基因小鼠T淋巴细胞的体外抗肿瘤作用显示文摘目的:研究小鼠T淋巴细胞在转染嵌合性T细胞受体基因后的体外抗肿瘤作用。方法:应用重组DNA技术,将HER2特异性嵌合性T-细胞受体构建入逆转录病毒载体;转入包装细胞后,收集病毒上清,转染小鼠T淋巴细胞,转基因后的小鼠T淋巴细胞分别与HER2阳性(SK-OV-3)或阴性(MCF-7)的肿瘤细胞系共培养,检测其细胞因子γ干扰素释放,51Cr释放法检测CTL评价其抗肿瘤效应。结果:所构建载体经酶切鉴定符合要求,乒乓法转染包装细胞系GP+E86,检测病毒滴度为1.2×106,Retronectin结合离心法转染经抗CD3/CD28单抗活化的小鼠T淋巴细胞,转染效率可达50%以上;转染嵌合性T细胞受体基因的T淋巴细胞与HER2阳性或阴性的肿瘤细胞系共培养后可检测到HER2特异性的细胞因子γ干扰素释放,51Cr释放法测CTL可见转染嵌合性T细胞受体基因T淋巴细胞对HER2阳性的肿瘤细胞具显著杀伤效应。结论:转染嵌合性T细胞受体基因的小鼠T淋巴细胞在体外可通过细胞因子释放和CTL效应发挥显著的抗肿瘤作用。 | 杨建民 Michael S Friedman 李峤 James J Mule Alfred E Chang Kevin T McDonagh | 2006 | 中国肿瘤生物治疗杂志2006，13，4： | 2 |
| 3 | Perioperative glucose management and outcomes in liver transplant recipients: A qualitative systematic review显示文摘AIM To investigate the relationship between post-liver transplantation(LT) glycemic control and LT outcomes. METHODS A qualitative systematic review on relevant prospective interventions designed to control glucose levels including insulin protocols. Studies investigating an association between glycemic control and post-LT outcomes such as mortality, graft rejection, and infection rate were reviewed. Pub Med, EMBASE, and other databases were searched through October 2016. RESULTS Three thousands, six hundreds and ninety-two patients from 14 studies were included. Higher mortality rate was seen when blood glucose(BG) ≥ 150 mg/dL(P = 0.05). BG ≥ 150 mg/dL also led to higher rates of infection. Higher rates of graft rejection were seen at BG > 200 mg/dL(P < 0.001). Mean BG ≥ 200 mg/dL was associated with more infections(P = 0.002).Nurse-initiated protocols and early screening strategies have shown a reduction in negative post-LT outcomes.CONCLUSION Hyperglycemia in the perioperative period is associated with poor post-LT outcomes. Only a few prospective studies have designed interventions aimed at managing post-LT hyperglycemia, post-transplant diabetes mellitus(PTDM) and their impact on post-LT outcomes. | Prani Paka Sarah R Lieber Ruth-Ann Lee Chirag S Desai Robert E Dupuis Alfred Sidney Barritt | 2018 | World Journal of Transplantation2018，8，3： | 2 |
| 4 | Corrosion kinetics of battery zinc alloys in electrolyte solutions 显示文摘 | BARBARA S MARIA C ALFRED N | 1998 | Journal of Power Sources1998，75，： | 1 |
| 5 | Antiendothelial cell antibodies in patients with Wegener's granulomatosis:prevalence and correlation with disease activity and manifestations显示文摘 | Jodi K Alfred D Sohail S | 2007 | The Journal of rheumatology2007，34，5： | 1 |
| 6 | Intracostal sutures decrease the pain of thoracotomy显示文摘 | Robert J Cerfolio Theolynn N Price Ayesha S Bryant Cynthia Sale Bass Alfred A Bartolucci | 2003 | The Annals of Thoracic Surgery2003，，2： | 1 |
| 7 | Unicast-based inference of network link delay distributions with finite mixture models 显示文摘 | Meng-Fu S Alfred O | 2003 | IEEE Trans on Signal Processing2003，51，8： | 1 |
| 8 | Long-term cancer-specific survival in patients with high-risk, non-muscle-invasive bladder cancer and tumour progression : a systematic review 显示文摘 | Van den Bosch S Alfred Witjes J | 2011 | Eur Urol2011，60，3： | 1 |
| 9 | The degrada- tion of arginine by Clostridium perfringens (BP6K) 显示文摘 | GILBERT C S MILAN A L ALFRED A T | 1952 | Journal of Biological Chemisty1952，198，2： | 1 |
| 10 | Effects and mechanisms of nonylphenol on corticosterone release in rat zona fasciculata-reticularis cells 显示文摘 | CHANG L L ALFRED WUN W S WANG P S | 2010 | Toxicol Sci2010，118，2： | 1 |
| 11 | Three-dimensional structure of the 'long' neurotoxin from cobra venom 显示文摘 | Malcolm D Wolfram S Alfred M | 1980 | Biochemistry1980，77，5： | 1 |
| 12 | Delayed neu- rologic sequelae resulting from epidemic diethylene glycol poisoning显示文摘 | Alfred S Coleman P Harris D | 2005 | Clin Toxicol (Phila)2005，43，3： | 1 |
| 13 | Delayed neurologic sequelae resulting from epidemic diethylene glycol poisoning显示文摘 | Alfred S Coleman P Harris D Wigmore T Stachowski E Graudins A | 2005 | Clin Toxicol ( Phila )2005，43，3： | 1 |
| 14 | Clementine Observations of the Aristarchus Region of the Moon显示文摘 | Alfred S M Mark S R Eric M E | 1994 | Science1994，266，： | 1 |
| 15 | Market Segmentation by Personal Values and Salient Product Attributes显示文摘 | Alfred S B | 1981 | Journal of Advertising Research1981，21，1： | 1 |
| 16 | Purification and characterization of histidinol dehydrogenase from cabbage显示文摘 | Atsuko N Alfred S | 1991 | Arch biochem Biophys1991，1，： | 1 |
| 17 | Alkane reactions with photoactivated decatungstate in neutral and acid solutipn molecular orbital theory显示文摘 | JEN S F ALFRED B HILL C L | 1992 | J Phys Chem1992，96，： | 1 |
| 18 | How much do specialistes have to learn from each other when they jointly develop radical product innovations 显示文摘 | CHRISTINA S ALFRED K | 2008 | Research Policy2008，，37： | 1 |
| 19 | Estimation of area burned by forest fires in Mediterranean countries: A remote sensing data mining perspective显示文摘 | Carmen Q Alfonso F M Alfred S | 2011 | Forest Ecology and Management2011，262，： | 1 |
| 20 | Nurses' preparedness and perceived competence in managing disasters显示文摘 | Baack S Alfred D | 2013 | J Nurs Scholarsh2013，45，3： | 1 |
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