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| 1 | Establishment of customized mouse stem cell lines by sequential nuclear transfer显示文摘治疗学的克隆,胚胎的干细胞(转换字符) 由此从原子转移(NT ) 被导出胚胎,可以在再生药的新时代起一个主要作用。Inthis 学习我们建立了四十原子转移 -- 从不同施主房间类型或段落的 NTembryos 被导出的转换字符(NT 转换字符) 线。我们发现 NT 转换字符能够形成胚胎植物或动物身体。另外, NT 转换字符表示了 pluripotency 干细胞标记试管内并且能区分进胚胎的纸巾体内。从施主房间能形成完整的术语的早经过 R1 的 NT 胚胎开发了小狗,而那些为为实时出生是必要的 reprogramming 从迟了的经过 R1 ES 施主房间失去了潜力。我们随后建立了近来从 NT 胚囊被开发的 sequentialNT-R1-ESC 线经过 R1 转换字符施主。然而,当在他们的早段落用作原子转移施主时,这些 NT-R1-ESC 排队,没能导致实时小狗。这显示用顺序的 NT 转换字符的治疗学的克隆过程不能救居住在以前的施主代的发展缺乏。 | Chunli Zhao Ruqiang Yao Jie Hao Chenhui Ding Yong Fan Xiangpeng Dai Wei Li Tang Hai Zichuan Liu Yang Yu Yingying Wang Xiaojun Hou Weizhi Ji Qi Zhou Alice Jouneau Fanyi Zeng Liu Wang | 2007 | Cell Research2007，17，1： | 1 |
| 2 | Efficient derivation of bovine embryonic stem cells needs more than active core pluripotency factors显示文摘 | Julien Maruotti Marta Mu?oz Severine A. Degrelle Enrique Gómez Claire Louet Carmen Díez Monforte Priscille Huot de Longchamp Vincent Brochard Isabelle Hue José Nestor Caama?o Alice Jouneau | 2012 | Mol Reprod Dev2012，，7： | 1 |
| 3 | Cloning efficiency following ES cell nuclear transfer is influenced by the methylation state of the donor nucleus altered by mutation of DNA methyltransferase 3a and 3b显示文摘The epigenetic state of donor cells plays a vital role in the nuclear reprogramming and chromatin remodel-ing of cloned embryos.In this study we investigated the effect of DNA methylation state of donor cells on the development of mouse embryos reconstructed with embryonic stem(ES)cell nuclei.Our results confirmed that deletion of the DNA methyltransferase 3a(Dnmt3a)and DNA methyltransferase 3b(Dnmt3b)distinctly decreases the level of DNA methylation in ES cells.In contrast to wild type ES cells(J1),Dnmt3a–/–3b–/–(DKO)and Dnmt3b–/–(3bKO)donor cells significantly elevated the percentage of embryonic stem cell nuclear transfer(ECNT)morula,blastocysts and postimplantation embryos(P<0.05).However,the efficiency of establish-ment of NT-ES cell lines derived from DKO reconstructed blastocysts was not improved,and the expression pattern of OCT4 and CDX2 in cloned blastocysts and postim-plantation embryos was not altered either.Our results suggest that the DNA methylation state of the donor nucleus is an important factor in regulation of the donor nuclear reprogramming. | Xiangpeng DAI Xiaoyang ZHAO Hai TANG Jie HAO Jean-Paul RENARD Qi ZHOU Alice JOUNEAU Liu WANG | 2010 | Frontiers in Biology2010，5，5： | 0 |
| 4 | 两性小鼠核移植胚胎干细胞系的建立及其在胚胎干细胞多能性评估中的研究显示文摘治疗性克隆的安全性与风险性是阻碍治疗性克隆发展的关键环节之一.由于受到社会伦理问题的限制,人胚胎干细胞的多能性检测只能通过畸胎瘤的方式鉴定,与传统的小鼠生殖系嵌合鉴定方式不同.但是两种多能性检测方式之间的区别还不清楚.本研究利用遗传突变两性小鼠心肌成纤维细胞作为核移植供体细胞,构建核移植胚胎,经过体外培养获得克隆囊胚,建立核移植胚胎干细胞系.对胚胎干细胞系的鉴定结果表明,两性小鼠核移植胚胎干细胞系表达所有小鼠胚胎干细胞系多能性及特异的分子标记,如碱性磷酸酶活性,Oct4,Nanog,SSEA-1等,干细胞注射到免疫缺陷小鼠(SCID)体内能够形成含有三胚层分化附属物的畸胎瘤组织,证明两性小鼠核移植胚胎干细胞系具有多能性.但通过囊胚注射的方式获得的二倍体嵌合小鼠经过交配不能获得生殖系嵌合的小鼠,因而两性小鼠核移植胚胎干细胞系并不具有全能性,说明体内形成三胚层分化附属物的畸胎瘤组织并不能替代传统的生殖系嵌合,提示在人治疗性克隆胚胎干细胞系多能性鉴定中,仍然需要更多、更严格的标准,进而最大程度地降低治疗性克隆临床应用的风险性. | 范勇 佟曼 赵春丽 丁晨晖 郝捷 吕卓 代相鹏 海棠 李雪梅 姚汝强 于洋 李赞东 王柳 ALICE Jouneau 周琪 | 2008 | 科学通报2008，53，21： | 0 |
| 5 | Comparative pluripotency analysis of mouse embryonic stem cells derived from wild-type and infertile hermaphrodite somatic cell nuclear transfer blastocysts显示文摘Therapeutic cloning, whereby embryonic stem cells (ESCs) are derived from patient-specific cloned blastocysts via somatic cell nuclear transfer (SCNT), holds great promise for treating many human diseases using regenerative medicine. Teratoma formation and germline transmission have been used to confirm the pluripotency of mouse stem cells, but human embryonic stem cells (hESCs) have not been proven to be fully pluripotent owing to the ethical impossibility of testing for germ line transmission, which would be the strongest evidence for full pluripotency. Therefore, formation of differentiated cells from the three somatic germ layers within a teratoma is taken as the best indicator of pluripotency in hESC lines. The possibility that these lines lack full multi-or pluripotency has not yet been evaluated. In this study, we established 16 mouse ESC lines, including 3 genetically defective nuclear transfer- ESC (ntESC) lines derived from SCNT blastocysts of infertile hermaphrodite F1 mice and 13 ntESC lines derived from SCNT blastocysts of normal F1 mice. We found that the defective ntESCs expressed all in vitro markers of pluripotency and could form teratomas that included derivatives from all three germ layers, but could not be transmitted via the germ line, in contrast with normal ntESCs. Our results indicate that teratoma formation assays with hESCs might be an insufficient standard to assess full pluripotency, although they do define multipotency to some degree. More rigorous standards are required to assess the safety of hESCs for therapeutic cloning. | FAN Yong TONG Man ZHAO ChunLi DING ChenHui HAO Jie LV Zhuo DAI XiangPeng HAI Tang LI XueMei YAO RuQiang YU Yang LI ZanDong WANG Liu ALICE Jouneau ZHOU Qi | 2008 | Chinese Science Bulletin2008，53，23： | 0 |
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