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您的检索式：作者名="Allen P.Liu"
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| 1 | On the gating of mechanosensitive channels by fluid shear stress显示文摘Mechanosensation is an important process in biological fluid–structure interaction. To understand the biophysics underlying mechanosensation, it is essential to quantify the correlation between membrane deformation,membrane tension, external fluid shear stress, and conformation of mechanosensitive(MS) channels. Smoothed dissipative particle dynamics(SDPD) simulations of vesicle/cell in three types of flow configurations are conducted to calculate the tension in lipid membrane due to fluid shear stress from the surrounding viscous flow. In combination with a simple continuum model for an MS channel, SDPD simulation results suggest that shearing adhered vesicles/cells is more effective to induce membrane tension sufficient to stretch MS channels open than a free shear flow or a constrictive channel flow. In addition, we incorporate the bilayer–cytoskeletal interaction in a two-component model to probe the effects of a cytoskeletal network on the gating of MS channels. | Zhangli Peng On Shun Pak Zhe Feng Allen P.Liu Yuan-Nan Young | 2016 | Acta Mechanica Sinica2016，32，6： | 1 |
| 2 | Synergistic and non-specific nucleic acid production by T7 RNA polymerase and Bsu DNA polymerase catalyzed by single-stranded polynucleotides显示文摘Point-of-care molecular diagnostic tests show great promise for providing accurate,timely results in lowinfrastructure healthcare settings and at home.The design space for these tests is limited by a variety of possible background reactions,which often originate from relatively weak promiscuous activities of the enzymes used for nucleic acid amplification.When this background signal is amplified alongside the signal of the intended biomarker,the dynamic range of the test can be severely compromised.Therefore,a detailed knowledge of potential side reactions arising from enzyme promiscuity can improve rational design of point-of-care molecular diagnostic tests.Towards this end,we report a previously unknown synergistic reaction between T7 RNA polymerase and Bsu DNA polymerase that produces nucleic acid in the presence of single-stranded DNA or RNA.This reaction occurs in the absence of any previously reported substrates for either polymerases and compromises a theoretical microRNA amplification scheme utilizing these polymerases. | Nicholas J.Emery Sagardip Majumder Allen P.Liu | 2018 | Synthetic and Systems Biotechnology2018，3，2： | 0 |
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