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| 1 | High-throughput RNA interference screens integrative analysis: Towards a comprehensive understanding of the virus-host interplay显示文摘Viruses are extremely heterogeneous entities; the size and the nature of their genetic information, as well as the strategies employed to amplify and propagate their genomes, are highly variable. However, as obligatory intracellular parasites, replication of all viruses relies on the host cell. Having co-evolved with their host for several million years, viruses have developed very sophisticated strategies to hijack cellular factors that promote virus uptake, replication, and spread. Identification of host cell factors(HCFs) required for these processes is a major challenge for researchers, but it enables the identification of new, highly selective targets for anti viral therapeutics. To this end, the establishment of platforms enabling genome-wide high-throughput RNA interference(HT-RNAi) screens has led to the identification of several key factors involved in the viral lifecycle. A number of genome-wide HT-RNAi screens have been performed for major human pathogens. These studies enable first inter-viral comparisons related to HCF requirements. Although several cellular functions appear to be uniformly required for the life cycle of most viruses tested(such as the proteasome and the Golgi-mediated secretory pathways), some factors, like the lipid kinase Phosphatidylinositol 4-kinase Ⅲα in the case of hepatitis C virus, are selectively required for individual viruses. However, despite the amount of data available, we are still far away from a comprehensive understanding of the interplay between viruses and host factors. Major limitations towards this goal are the low sensitivity and specificity of such screens, resulting in limited overlap between different screens performed with the same virus. This review focuses on how statistical and bioinformatic analysis methods applied to HTRNAi screens can help overcoming these issues thus increasing the reliability and impact of such studies. | Sandeep Amberkar Narsis A Kiani Ralf Bartenschlager Gualtiero Alvisi Lars Kaderali | 2013 | World Journal of Virology2013，2，2： | 9 |
| 2 | Hepatoprotective activity of Terminalia paniculata against paracetamol induced hepatocellular damage in Wistar albino rats显示文摘Objective:To evaluate the hepatoprotective activity of Terminalia paniculata against paracetamol induced hepatic damage in rats.Methods:The plant material was shade dried, powdered and extracted with ethanol.Liv 52 and silymarin were used as standard drugs and 2%gum acacia as a control(vehicle).Alteration in the levels of biochemical markers of hepatic damage like AST,ALT,ALP and lipid peroxides were tested,and phytochemical tests were also performed.Results:Paracetamol(2 g/kg) increased the serum levels of alanine aminotransfer (ALT),aspartate aminotransferase(AST),alkaline phosphatase(ALP) and the lipid peroxides. Treatment of Liv 52,silymarin and ethanolic extract of Terminalia paniculata(200 mg/kg) altered levels of biochemical marker and showed significant hepatoprotective activity.Ethanolic extract revealed the presence of phenolic compound and flavanoids.Our findings suggested that ethanolic bark extract of Terminalia paniculata possessed hepatoprotective activity in a dose dependent manner.Conclusions:Terminalia paniculata possesses hepatoprotective activity.It could be an effective and promising preventive agent against PCT induced hepatotoxicity. | Eesha BR Mohanbabu Amberkar V Meena Kumari K Sarath babu Vijay M Lalit M Rajput R | 2011 | Asian Pacific Journal of Tropical Medicine2011，4，6： | 5 |
| 3 | Wound healing activity of aqueous extract of Crotalaria verrucosa in Wistar albino rats显示文摘Objective:To evaluate the wound healing effect of aqueous extract of Crotalaria verrucosa(C. verrucosa) in rats.Methods:Three wound models including incision,excision and dead space wounds were used in this study.The parameters studied were breaking strength in incision models,granulation tissue dry weight,breaking strength and hydroxyproline content in dead space wounds,percentage of wound contraction and period of epithelialization in excision wound model.Results:Two doses of the extract with and without dexamethasone showed significant increases in mean hydroxyproline,total protein content and dry weight of granulation tissue but it was higher with dose 800 mg/kg comparing with the control.The dexamethasone treated group showed a significant(P<0.001) reduction in the wound breaking strength when compared to control group in incision type of wound model.Coadministration of C.verrucosa with dexamethasone significantly(P<0.001) increased the breaking strength compared to the dexamethasone treated only group.In excision wound model,the percentage of the wound contraction was significantly(P<0.01) increased by two doses of test extract on all the days except the lower dose which exhibited only on 12th,16th days of drug treatment and it also reversed the dexamethasone suppressed wound contraction.It significantly(P<0.001) reduced the time required for epithelialization and reversed the epithelialization delaying effect of dexamethasone (P<0.001).Conclusions:C.verrucosa was found to possess significant wound healing property. This was evident by decrease in the period of epithelialization,increase in the rate of wound contraction,skin breaking strength,and granulation tissue dry weight content.Hence C. verrucosa could be a good wound healing agent. | Meena Kumari Eesha BR Mohanbabu Amberkar Sarath babu Rajshekar Neelesh Kumar | 2010 | Asian Pacific Journal of Tropical Medicine2010，3，10： | 3 |
| 4 | Symbolic notation of planetary gear trains by canonical number approach显示文摘 | MADAN S R AMBERKAR A G JAIN R C | 2000 | International Journal of Mechanical Engineering Education2000，28，1： | 1 |
| 5 | Diagnostic Development for an Electric Power Steering System 显示文摘 | Amberkar S Kushion M Eschtruth K | 2000 | SAE technical paper series2000，1，： | 1 |
| 6 | A Control System Methodology for Steer by Wire System显示文摘 | Sanket Amberkar Farhad Bolourchi | 2004 | SAE TECHNICAL PAPER SERIES2004，，1： | 1 |
| 7 | Diagnostic Development for an Electric Power Steering System显示文摘 | Sanket Amberkar Mark Kushion Kirt Eschtruth etc | 2000 | SAE Technical Paper Series2000，，1： | 1 |
| 8 | Diagnostic Development for an Electric Power Steering System 显示文摘 | Sanket Amberkar Mark Kushion Kirt Eschtruth Farhad Bolourchi | 2000 | Proc of SAE 2000 World Congress2000，，： | 1 |
| 9 | Symbolic notation of planetary gear trains by canonical number approach显示文摘 | MADAM S R AMBERKAR A G JAIN R C | 2000 | International Journal of Mechanical Engineering Education2000，28，1： | 1 |
| 10 | Symbolic notation of planetary gear trains by canonical number approach显示文摘 | Madan S R Amberkar A G Jain R C | 2000 | International Journal of Mechanical Engineering Education2000，28，1： | 1 |
| 11 | Synthesis, characterization and lattice thermal expansion of some compounds in the system GdCexZr2_xO显示文摘 | PATWE S J AMBERKAR B R TYAGI A K | 2005 | Journal of Alloys and Compounds2005，389，： | 1 |
| 12 | A Control System Methodology for Steer by Wire System显示文摘 | Sanket Amberkar Farhad Bolourchi Jon Demerly Scott Millsap | 2004 | SAE Technical Paper Series2004，，1： | 1 |
| 13 | A Control System Methodology for Steer byWire Systems显示文摘 | AMBERKAR S BOLOURCHI F DEMERLY J | 2004 | SAE Paper2004，，： | 1 |
| 14 | A Control System Methodology for Steer by Wire System显示文摘 | Sanket Amberkar Farhad Bolourchi | 2004 | SAE Technical Paper Series2004，，： | 1 |
| 15 | Delphi automotive systems, diagnostic development for an electric power steering system显示文摘 | Sanket Amberkar Mark Kushion Kirt Eschtruth | 2000 | SAE Paper2000，109，： | 1 |
| 16 | Diagnostic Development for an Electric Power Steering System显示文摘 | Sanket Amberkar Mark Kushion Kirt Eschtruth | 2000 | Proc of SAE 2000 World Congress2000，，： | 1 |
| 17 | Symbolic notation of planetary gear trains by canonical number apporach显示文摘 | Madan S R Amberkar A G Jain R C | | 0，，01： | 1 |
| 18 | Delphi Automotive Systems, Diagnostic Development for an Electric Power Steering System 显示文摘 | Sanket Amberkar Mark Kushion Kirt Eschtruth Farhad Bolourchi | 2000 | SAE Technical Paper Series2000，，： | 1 |
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