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| 1 | Relationship between Fusobacterium nucleatum,inflammatory mediators and microRNAs in colorectal carcinogenesis显示文摘AIM To examine the effect of Fusobacterium nucleatum(F. nucleatum) on the microenvironment of colonic neoplasms and the expression of inflammatory mediators and microRNAs(miRNAs).METHODS Levels of F. nucleatum DNA, cytokine gene mRNA(TLR2, TLR4, NFKB1, TNF, IL1 B, IL6 and IL8), and potentially interacting miRNAs(miR-21-3p, miR-22-3p, mi R-28-5p, miR-34a-5p, miR-135b-5p) were measured by quantitative polymerase chain reaction(qPCR) TaqMan? assays in DNA and/or RNA extracted from the disease and adjacent normal fresh tissues of 27 colorectal adenoma(CRA) and 43 colorectal cancer(CRC) patients. KRAS mutations were detected by direct sequencing and microsatellite instability(MSI) status by multiplex PCR. Cytoscape v3.1.1 was used to construct the postulated miRNA:mRNA interaction network.RESULTS Overabundance of F. nucleatum in neoplastic tissue compared to matched normal tissue was detected in CRA(51.8%) and more markedly in CRC(72.1%). We observed significantly greater expression of TLR4, IL1 B, IL8, and miR-135 b in CRA lesions and TLR2, IL1 B, IL6, IL8, mi R-34 a and miR-135 b in CRC tumours compared to their respective normal tissues. Only two transcripts for miR-22 and miR-28 were exclusively downregulated in CRC tumour samples. The mRNA expression of IL1 B, IL6, IL8 and miR-22 was positively correlated with F. nucleatum quantification in CRC tumours. The mRNA expression of miR-135 b and TNF was inversely correlated. The miRNA:mRNA interaction network suggested that the upregulation of miR-34 a in CRC proceeds via a TLR2/TLR4-dependent response to F. nucleatum. Finally, KRAS mutations were more frequently observed in CRC samples infected with F. nucleatum and were associated with greater expression of miR-21 in CRA, while IL8 was upregulated in MSI-high CRC.CONCLUSION Our findings indicate that F. nucleatum is a risk factor for CRC by increasing the expression of inflammatory mediators through a possible mi RNA-mediated activation of TLR2/TLR4. | Marcela Alcantara Proenca Joice Matos Biselli Maysa Succi Fábio Eduardo Severino Gustavo Noriz Berardinelli Alaor Caetano Rui Manuel Reis David J Hughes Ana Elizabete Silva | 2018 | World Journal of Gastroenterology2018，24，47： | 15 |
| 2 | Concordance of non-invasive mechanical and serum tests for liver fibrosis evaluation in chronic hepatitis C显示文摘AIM To determine the sensitivity and specificity of liver stiffness measurement(LSM) and serum markers(SM) for liver fibrosis evaluation in chronic hepatitis C.METHODS Between 2012 and 2014,81 consecutive hepatitis C virus(HCV) patients had METAVIR score from liver biopsy compared with concurrent results from LSM [transient elastography(TE) [FibroS can~/ARFI technology(Virtual Touch~)] and SM [FIB-4/aspartate aminotransferase-toplatelet ratio index(APRI)].The diagnostic performance of these tests was assessed using receiver operating characteristic curves.The optimal cut-off levels of each test were chosen to define fibrosis stages F ≥ 2,F ≥ 3 and F = 4.The Kappa index set the concordance analysis.RESULTS Fifty point six percent were female and the median age was 51 years(30-78).Fifty-six patients(70%) weretreatment-na?ve.The optimal cut-off values for predicting F ≥ 2 stage fibrosis assessed by TE were 6.6 kP a,for acoustic radiation force impulse(ARFI) 1.22 m/s,for APRI 0.75 and for FIB-4 1.47.For F ≥ 3 TE was 8.9 kP a,ARFI was 1.48 m/s,APRI was 0.75,and FIB-4 was 2.For F = 4,TE was 12.2 kP a,ARFI was 1.77 m/s,APRI was 1.46,and FIB-4 was 3.91.The APRI could not distinguish between F2 and F3,P = 0.92.The negative predictive value for F = 4 for TE and ARFI was 100%.Kappa index values for F ≥ 3 METAVIR score for TE,ARFI and FIB-4 were 0.687,0.606 and 0.654,respectively.This demonstrates strong concordance between all three screening methods,and moderate to strong concordance between them and APRI(Kappa index = 0.507).CONCLUSION Given the costs and accessibility of LSM methods,and the similarity with the outcomes of SM,we suggest that FIB-4 as well as TE and ARFI may be useful indicators of the degree of liver fibrosis.This is of particular importance to developing countries. | Denise C Paranaguá-Vezozzo Adriana Andrade Daniel F C Mazo Vinicius Nunes Ana L Guedes Taisa G Ragazzo Renata Moutinho Lucas S Nacif Suzane K Ono Venancio A F Alves Flair J Carrilho | 2017 | World Journal of Hepatology2017，9，8： | 7 |
| 3 | Guiexperts显示文摘 | Irwig L Tosteson ANA Gatsonis C Lau J Colditz G Chalmers TC Mosteller F | 1992 | JAMA1992，268，： | 1 |
| 4 | Captopril improves cerebrovascular structure and function in old hypertensive rats显示文摘 | Dupuis F Atkinson J Limiana P Chillon J M | 2005 | Br J Pharmacol2005，144，3： | 1 |
| 5 | In vitro assessment of Metarhizium anisopliae isolates to control the cattle tick Boophilus microplus 显示文摘 | Ana Paula G F habajara da S V J Aoi M | 2000 | Veterinary Parasitology2000，91，： | 1 |
| 6 | Recent in- sights into the pathogenesis of bacterial sepsis显示文摘 | Anas A A Wiersinga W J de Vos A F | 2010 | Neth J Med2010，68，4： | 1 |
| 7 | Effectiveness of oral rinse with Chlorhex- idine in preventing nosocomial respiratory tract infec- tions among intensive care unit patients显示文摘 | Bellissimo-Rodrigues F Bellissimo-RodriguesW T Vi- ana J M | 2009 | Infect Con- trol Hosp Epidemiol2009，30，10： | 1 |
| 8 | Blood vessel normalization in the hamster oral cancer model for experimental cancer therapy studies显示文摘 | Ana J Molinari A J Romina F | 2012 | Anticancer Res2012，32，7： | 1 |
| 9 | Dietary fiber from vegetable Products as source of functional ingredients 显示文摘 | Rocio R Ana J Juan F B | 2006 | Food Science & Technology2006，，17： | 1 |
| 10 | Structure of calcium silicate hydrates formed in alkaline-activated slag: influence of the type of alkalineactivator显示文摘 | Ana F J Francisca P Isabel S | | Journal of the American Ceramic Society0，，： | 1 |
| 11 | Phagocytic NADPH oxidase over activity underlies oxidative stress in metabolic syndrome显示文摘 | ANA F GORKA S J MARE U | 2006 | Diabeles2006，55，1： | 1 |
| 12 | Dietary fibre from vegetable products as source of functional ingredients 显示文摘 | Rocio R Ana J Juan F | 2006 | Food Science & Technology2006，17，1： | 1 |
| 13 | Dietary fiber from vegetable products as source of functional ingredients 显示文摘 | Rocio R Ana J Juan F B | 2006 | Trends in Food Science&Technology2006，17，1： | 1 |
| 14 | Evaluation of synthetic iron (Ⅲ)-chelates (EDDHA/Fe3+,EDDHMA/Fe3 and the novel EDDHSA/Fe3+) to correct iron chlorosis显示文摘 | Ana A F Sonia G M Juan J L | 2005 | European Journal of Agronomy2005，22，： | 1 |
| 15 | Chitosan nanoparticles as delivery systems for doxorubicin显示文摘 | Kevin A J Marie P F Ana M | | 0，，： | 1 |
| 16 | Dietary fiber from vegetable products as source of functional ingredients显示文摘 | Rocio R Ana J Juan F B | 2006 | Trends in Food Science & Technology2006，17，1： | 1 |
| 17 | In vitro assess-ment of Metarhizium anisopliaeisolates to control the cattle tickBoophilus microplus 显示文摘 | Ana Paula G F Itabajara da S V J Aoi M | 2000 | Veterinary Parasitology2000，94，11： | 1 |
| 18 | Erythrocyte invasion by Babesia boris merozoites is inhibited by polyclonal antisera directed against peptides derived from a homologue of Plasmodium falciparum apical membrane antigen 显示文摘 | FASILA R G ANA P Y FRITS F J | 2004 | Infect Immun2004，72，5： | 1 |
| 19 | Displacement and load transfer mechanism of geogrids under pullout condition 显示文摘 | Ana Cristina C F Sieira Denise M S Gerscovich Alberto S F J Sayao | 2009 | Geotextiles and Geomembranes2009，27，4： | 1 |
| 20 | Glutathione re-ductase activity and isoforms in leaves and roots of wheatplants subjected to cadmium stress显示文摘 | GustavoG Y Ana J F A Diego M St | 2007 | Phytochemistry2007，，68： | 1 |
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