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| 1 | hsa-miR-29c and hsa-miR-135b differential expression aspotential biomarker of gastric carcinogenesis显示文摘AIM: To investigate the expression profiles of hsa-mi R-29 c and hsa-mi R-135 b in gastric mucosal samples and their values as gastric carcinogenesis biomarkers. METHODS: The expression levels of hsa-mi R-29 c and hsa-mi R-135 b in normal gastric mucosa, non-atrophic chronic gastritis, intestinal metaplasia and intestinaltype gastric adenocarcinoma were analysed using quantitative real-time PCR. The difference between hsa-mi R-29 c and hsa-mi R-135 b expression profiles in the grouped samples was evaluated by ANOVA and Student's t-test tests. The results were adjusted for multiple testing by using Bonferroni's correction. P values ≤ 0.05 were considered statistically significant. To evaluate hsa-mi R-29 c and hsa-mi R-135 b expressions as potential biomarkers of gastric carcinogenesis, we performed a receiver operating characteristic curve analysis and the derived area under the curve, and a Categorical Principal Components Analysis. In silico identification of the genetic targets of hsa-mi R-29 c and hsa-mi R-135 b was performed using different prediction tools, in order to identify possible genes involved in gastric carcinogenesis.RESULTS: The expression levels of hsa-mi R-29 c were higher in normal gastric mucosal samples, and decreased progressively in non-atrophic chronic gastritis samples, intestinal metaplasia samples and intestinal-type gastric adenocarcinoma samples. The expression of hsa-mi R-29 c in the gastric lesions showed that non-atrophic gastritis have an intermediate profile to gastric normal mucosa and intestinal-type gastric adenocarcinoma, and that intestinal metaplasia samples presented an expression pattern similar to that in intestinal-type gastric adenocarcinoma. This micro RNA(mi RNA) has a good discriminatory accuracy between normal gastric samples and(1) intestinal-type gastric adenocarcinoma; and(2) intestinal metaplasia, and regulates the DMNT3 A oncogene. hsa-mi R-135 b is up-regulated in non-atrophic chronic gastritis and intestinal metaplasia samples and down-regulated in normal gastric mucosa and intestinal-type gastric adenocarcinoma samples. Non-atrophic chronic gastritis and intestinal metaplasia are significantly different from normal gastric mucosa samples. hsa-mi R-135 b expression presented a greater discriminatory accuracy between normal samples and gastric lesions. This mi RNA was associated with Helicobacter pylori presence in non-atrophic chronic gastritis samples and regulates the APC and KLF4 tumour suppressor genes.CONCLUSION: Our results provide evidence of epigenetic alterations in non-atrophic chronic gastritis and intestinal metaplasia and suggest that hsa-mi R-29 c and hsa-mi R-135 b are promising biomarkers of gastric carcinogenesis. | Amanda Ferreira Vidal Aline MP Cruz Leandro Magalhães Adenilson L Pereira Ana KM Anaissi Nélisson CF Alves Paulo JBS Albuquerque Rommel MR Burbano Samia Demachki Ândrea Ribeiro-dos-Santos | 2016 | World Journal of Gastroenterology2016，22，6： | 7 |
| 2 | Increase in ornithine decarboxylase activity in the positive inotropism induced by androgens in isolated left atrium of the rat显示文摘 | Carmen B Jose MR Ana BV | 2001 | Eur J Pharmacol2001，422，13： | 1 |
| 3 | Leiomioma de es6fago trata- miento per abordaje mlnimamente invasivo (laparoscopia-toracoseo- pia) 显示文摘 | Maria JM Ana GN Alfonso MR | 2012 | Cirugia Espaola2012，90，5： | 1 |
| 4 | Mecha- nism in photodynamic therapy Part three: Photosensifi- zers pharmacokinetics,bioditribution,tumor localization and modes of tumordestruction 显示文摘 | Castano Ana P Tatiana N Demidova MR | 2005 | Photodiagnosis Photodyn Ther2005，2，2： | 1 |
| 5 | Kinetics of adsorption of whey proteins and hydroxypropyl-methyl-cellulose mixtures at the air-water interface 显示文摘 | Prez Oscar E Snchez Cecilio Carrera Pilosof Ana MR | 2009 | Journal of Colloid and Interface Science2009，336，2： | 1 |
| 6 | Increase in ornithine decarboxylase activity in the positive inotropism induced by androgens in isolated left atrium of the rat显示文摘 | Carmen B Jose MR Ana BV | 2001 | Eur J Pharmacol2001，422，12： | 1 |
| 7 | Quantitative analysis of argyrophilic nucleolar organizer regions and epidermal growth factor receptor in ameloblastomas 显示文摘 | Payeras MR Sant'Ana Filho M Lauxen IS | 2007 | J Oral Pathol Med2007，36，2： | 1 |
| 8 | Endocrine disruptors:from wingspread to environmental developmental biology显示文摘 | Caroline M Beverly SM Ana MR | 2003 | J Steroid Biochem2003，83，： | 1 |
| 9 | Major histocompatibility complex ( MHC ) class 11 but not MHC class I molecules are required for efficient control of Strongyloides venezuelensis infection in mice 显示文摘 | ROSANGELA MR NEIDE MS ANA LRG et ol | 2009 | Immunology2009，128，1： | 1 |
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