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| 1 | Single nucleotide polymorphisms of OCTN1, OCTN2, and DLG5 genes in Greek patients with Crohn's disease显示文摘AIM: To validate novel single nucleotide polymorphisms (SNPs) in Greek patients with Crohn's disease (CD).METHODS: A total of 120 patients with CD, 85 patients with UC, and 100 unrelated healthy controls were genotyped. Genotyping was performed by allele-specific PCR or by PCR-RFLP analysis.RESULTS: Our results showed that the 1672T and -207C alleles were obviously over-represented in CD patients only (P<0.01 and P<0.05, respectively) compared to the control population. The G113A polymorphism was completely absent in our studied population. The odds ratio for the carriage of the TC haplotype was 2.21 for CD patients as compared with controls. Additionally, the frequency of the TC haplotype was increased in patients with ileocolitis or colitis, and was mainly associated with the fibrostenotic phenotype of the disease. Furthermore, when the TC haplotype was compared jointly with the carriage of at least one mutation of the NOD2/CARD15 gene, there was an increased risk for CD, but not for UC, compared to controls. Regarding the location of the disease, the concomitant presence of the TC haplotype and NOD2/CARD15 mutations was mainly associated with ileocolitis or ileitis. CONCLUSION: Collectively, our results suggest that the 1672T variant of the OCTN1 gene and the -207C variant of the OCTN2 gene represent risk factors for CD in the Greek population. | Maria Gazouli Gerassimos Mantzaris Athanassios J Archimandritis George Nasioulas Nicholas P Anagnou | 2005 | World Journal of Gastroenterology2005，11，47： | 12 |
| 2 | NOD2/CARD15 , ATG16L1 and IL23R gene polymorphisms and childhood-onset of Crohn’s disease显示文摘AIM: To assess whether the polymorphisms of NOD2/ CARD15 , autophagy-related 16-like 1 (ATG16L1 ), and interleukin-23 receptor (IL23R ) genes play a more critical role in the susceptibility of childhood-onset than in adult-onset Crohn’s disease (CD). METHODS: Polymorphisms R702W, G908R, and 3020insC of NOD2/CARD15 ; rs2241880 A/G of ATG16L1 , and rs11209026 (R381Q) of IL23R gene were assessed in 110 childhood-onset CD, 364 adult-onset CD, and 539 healthy individuals. Analysis of polymorphisms R702W, G908R, and 3020insC of NOD2/CARD15 genotyping was performed by allele specific polymerase chain reaction (PCR) or by PCR-restriction fragment length polymor-phism analysis. The polymorphisms rs2241880 A/G of the ATG16L1 , and rs11209026 (R381Q) of the IL23R gene in the children’s cohort were genotyped by PCR and melting curve analysis whereas adult group genotyping was performed using the Affymetrix Genome-Wide Human SNP Array 5.0 (500K). RESULTS: The 3020insC allele in NOD2/CARD15 was significantly higher in childhood than in adult-onset CD (P = 0.0067). Association with at least 1 NOD2/CARD15 variant was specific for ileal disease (with or without co- lonic involvement). Even if the frequency of G allele of the rs2241880 ATG16L1 polymorphism was increased in both paediatric and adult CD patients compared to con- trols (P = 0.017 and P = 0.001, respectively), no difference was observed between the childhood and the adult cohort. The rare Q allele of IL23R rs11209026 polymorphism was underrepresented in both paediatric and adult CD cases (P = 0.0018 and P = 0.04, respectively) and no difference was observed between the childhood and the adult cohort. The presence of the rs2241880 ATG16L1 and rs11209026 IL23R polymorphisms did not influence disease phenotype. CONCLUSION: Polymorphism 3020insC in NOD2/ CARD15 occurs statistically significantly more often in patients with childhood-onset CD than in patients with adult-onset CD. The ATG16L1 and IL23R variants are associated with susceptibility to CD, but not earlyonset disease. | Maria Gazouli Ioanna Pachoula Ioanna Panayotou Gerassimos Mantzaris George Chrousos Nicholas P Anagnou Eleftheria Roma-Giannikou | 2010 | World Journal of Gastroenterology2010，16，14： | 7 |
| 3 | Development of a quantum-dot-labelled magnetic immunoassay method for circulating colorectal cancer cell detection显示文摘AIM:To detect of colorectal cancer(CRC) circulating tumour cells(CTCs) surface antigens,we present an assay incorporating cadmium selenide quantum dots(QDs) in these paper.METHODS:The principle of the assay is the immunomagnetic separation of CTCs from body fluids in conjunction with QDs,using specific antibody biomarkers:epithelial cell adhesion molecule antibody,and monoclonal cytokeratin 19 antibody.The detection signal was acquired from the fluorescence signal of QDs.For the evaluation of the performance,the method under study was used to isolate the human colon adenocarcinoma cell line(DLD-1) and CTCs from CRC patients' peripheral blood.RESULTS:The minimum detection limit of the assay was defined to 10 DLD-1 CRC cells/mL as fluorescence was measured with a spectrofluorometer.Fluorescenceactivated cell sorting analysis and Real Time RT-PCR,they both have also been used to evaluate the performance of the described method.In conclusion,we developed a simple,sensitive,efficient and of lower cost(than the existing ones) method for the detection of CRC CTCs in human samples.We have accomplished these results by using magnetic bead isolation and subsequent QD fluorescence detection.CONCLUSION:The method described here can be easily adjusted for any other protein target of either the CTC or the host. | Maria Gazouli Anna Lyberopoulou Pericles Pericleous Spyros Rizos Gerassimos Aravantinos Nikolaos Nikiteas Nicholas P Anagnou Efstathios P Efstathopoulos | 2012 | World Journal of Gastroenterology2012，18，32： | 6 |
| 4 | Gestational diabetes exhibits lack of carnitine deficiency despite relatively low carnitine levels and alterations in ketogenesis 显示文摘 | Pappa K I Anagnou N P Salanmlekis E | 2005 | J Matem Fetal Neonatal Med2005，17，1： | 1 |
| 5 | Towards More Successful Gene Therapy Clinical Trials for β-Thalassemia显示文摘 | E. Drakopoulou E. Papanikolaou M. Georgomanoli N. P. Anagnou | 2013 | Current Molecular Medicine2013，，8： | 1 |
| 6 | 5 -azacytidine selectively increases gamma - globin synthesis in a patient with beta^+ thalassemia 显示文摘 | Ley T J DeSimone J Anagnou NP | 1982 | N Engl J Med1982，307，24： | 1 |
| 7 | Emerging issues of the expression profiling technologies for the study of gynecologic cancer显示文摘 | Pappa K I Anagnou N P | 2005 | Am J Obstet Gynecol2005，193，： | 1 |
| 8 | Amniotic fluid and amniotic membrane stem cells : marker discovery 显示文摘 | Roubelakis MG Trohatou O Anagnou NP | 2012 | Stem Cells Int2012，2012，10： | 1 |
| 9 | Novel sources of fetal stem cells: wheredo they fit on the developmental continuum 显示文摘 | Pappa KI Anagnou NP | 2009 | Regen Med2009，4，42： | 1 |
| 10 | 5-HT2A Receptor Gene Polymorphisms and Irritable Bowel Syndrome显示文摘 | Theofano Markoutsaki Theodoros Karantanos Maria Gazouli Nicholas P. Anagnou Dimitrios G. Karamanolis | 2011 | Journal of Clinical Gastroenterology2011，，6： | 1 |
| 11 | Effect of storage temperature on the behaviour of Escherichia coli O157 :H7 and Salmonella enterica serotype Typhimurium on salad vegetables 显示文摘 | Likotrafiti E Anagnou M Lampiri P | 2014 | Journal of Food Research2014，3，2： | 1 |
| 12 | Gestational diabetes mellitus shares polymorphisms of genes associated with insulin resistance and type 2 diabetes in the Greek population显示文摘 | Kalliopi I. Pappa Maria Gazouli Konstantinos Economou George Daskalakis Eleni Anastasiou Nicholas P. Anagnou Aristides Antsaklis | 2011 | Gynecological Endocrinology2011，，4： | 1 |
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