| 1 | Re-evaluating the role of epithelial-mesenchymal-transition in cancer progression显示文摘Epithelial-mesenchymal transition(EMT) and mesenchymal-epithelial transition(MET) are essential for embryonic development and also important in cancer progression. In a conventional model, epithelial-like cancer cells transit to mesenchymal-like tumor cells with great motility via EMT transcription factors; these mesenchymallike cells migrate through the circulation system, relocate to a suitable site and then convert back to an epithelial-like phenotype to regenerate the tumor. However, recent findings challenge this conventional model and support the existence of a stable hybrid epithelial/mesenchymal(E/M) tumor population. Hybrid E/M tumor cells exhibit both epithelial and mesenchymal properties, possess great metastatic and tumorigenic capacity and are associated with poorer patient prognosis. The hybrid E/M model and associated regulatory networks represent a conceptual change regarding tumor metastasis and organ colonization. It may lead to the development of novel treatment strategies to ultimately stop cancer progression and improve disease-free survival. | Andrew Sulaiman Zemin Yao Lisheng Wang | 2018 | The Journal of Biomedical Research2018，32，2： | 4 |
| 5 | 水动力尾静脉注射介导Akt/β-catenin/Yap质粒转染建立小鼠肝癌模型显示文摘目的通过应用水动力尾静脉注射(hydrodynamic tail vein injection,HTVi)Sleeping Beauty(SB)质粒及促癌基因质粒,构建一种新型小鼠肝癌模型。方法6周龄雄性C57BL/6J小鼠,按照体重随机分为对照组和模型组。将冻存的SB、Akt、β-catenin、Yap质粒转化入感受态E.coli DH5α大肠杆菌,于LB培养基扩增后提取质粒。每只模型组小鼠尾静脉快速注射上述质粒混合溶液2 mL,包含SB质粒5μg,Akt、β-catenin、Yap质粒分别15μg,对照组小鼠给予等量PBS尾静脉注射。继续饲养8周后处死小鼠,观察小鼠情况,记录体重、肝重。观察小鼠肝脏病理学变化,并应用Western blot法检测小鼠肝组织中Akt、β-catenin、Yap基因表达水平。结果高压注射质粒8周后,对照组小鼠活动度好,皮毛光亮;小鼠肝脏形态正常;肝组织HE染色显示,对照组小鼠肝小叶结构完整。模型组小鼠活动减少,皮毛无光泽。与对照组比较,模型组小鼠体重下降、肝重增加、肝体比显著上调;模型组小鼠肝脏体积增大,质地较硬,表面出现颗粒状、结节样改变;肝组织HE染色显示,模型组小鼠肝组织结构紊乱,并出现多个肿瘤结节。此外,与对照组相比,模型组小鼠肝组织中Akt、β-catenin、Yap基因表达均明显上调。结论应用水动力尾静脉注射SB质粒及Akt/β-catenin/Yap质粒的方法可成功建立小鼠肝癌模型,为肝癌发病机制及药物研发提供快速、经济的动物模型。 | 蒋雨薇 宋海燕 李怡萍 汤凯悦 徐娇雅 SULAIMAN Andrew MCGARRY Sarah WANG Lisheng 郑培永 | 2020 | 临床与实验病理学杂志2020，36，10： | 0 |
您所在的机构暂未开通此部分的功能，请联系管理员