| 1 | CRISPR-detector:fast and accurate detection,visualization,and annotation of genome-wide mutations induced by genome editing events显示文摘The leading-edge CRISPR/CRISPR-associated technology is revolutionizing biotechnologies through genome editing.To track on/off-target events with emerging new editing techniques,improved bioinformatic tools are indispensable.Existing tools suffer from limitations in speed and scalability,especially with whole-genome sequencing(WGS)data analysis.To address these limitations,we have developed a comprehensive tool called CRISPR-detector,a web-based and locally deployable pipeline for genome editing sequence analysis.The core analysis module of CRISPR-detector is based on the Sentieon TNscope pipeline,with additional novel annotation and visualization modules designed to fit CRISPR applications.Co-analysis of the treated and control samples is performed to remove existing background variants prior to genome editing.CRISPR-detector offers optimized scalability,enabling WGS data analysis beyond Browser Extensible Data file-defined regions,with improved accuracy due to haplotype-based variant calling to handle sequencing errors.In addition,the tool also provides integrated structural variation calling and includes functional and clinical annotations of editing-induced mutations appreciated by users.These advantages facilitate rapid and efficient detection of mutations induced by genome editing events,especially for datasets generated from WGS.The web-based version of CRISPR-detector is available at http://gffzz984746bd41ee48d7s9uox0bvxkx6q6vfn.ffgz.tsg.suse.edu.cn/crispr-detector,and the locally deployable version is available at http://gffzz188fe103f8f1460as9uox0bvxkx6q6vfn.ffgz.tsg.suse.edu.cn/hlcas/CRISPR-detector. | Lei Huang Dan Wang Haodong Chen Jinnan Hu Xuechen Dai Chuan Liu Anduo Li Xuechun Shen Chen Qi Haixi Sun Dengwei Zhang Tong Chen Yuan Jiang | 2023 | Journal of Genetics and Genomics2023，50，8： | 0 |
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