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| 1 | Alleviated mucosal and neuronal damage in a rat model of Crohn's disease显示文摘AIM:To establish a rat model suitable to investigate the repetitive relapsing inflammations(RRI)characteristic to Crohn’s disease.METHODS:Colitis was induced by 2,4,6-trinitrobenzenesulfonic acid(TNBS).RRI were mimicked by repeating administrations of TNBS.Tissue samples were taken from control,once,twice and three times treated rats from the inflamed and adjacent non-inflamed colonic segments at different timepoints during the acute intestinal inflammation.The means of the ulcerated area were measured to evaluate the macroscopic mu-cosal damage.The density of myenteric neurons was determined on whole mounts by Hu C/Hu D immunohistochemistry.Heme oxygenase-1(HO-1)expression was evaluated by molecular biological techniques.RESULTS:TNBS-treated rats displayed severe colitis,but the mortality was negligible,and an increase of body weight was characteristic throughout the experimental period.The widespread loss of myenteric neurons,and marked but transient HO-1 up-regulation were demonstrated after the first TNBS administration.After repeated doses the length of the recovery time and extent of the ulcerous colonic segments were markedly decreased,and the neuronal loss was on a smaller scale and was limited to the inflamed area.HO-1 m RNA level was notably greater than after a single dose and overexpression was sustained throughout the timepoints examined.Nevertheless,the HO-1protein up-regulation after the second TNBS treatment proved to be transient.Following the third treatment HO-1 protein expression could not be detected.CONCLUSION:Experimentally provoked RRI may exert a protective preconditioning effect against the mucosal and neuronal damage.The persistent up-regulation of HO-1 m RNA expression may correlate with this. | Petra Talapka Lajos István Nagy Alexandra Pál Marietta Zita Poles Anikó Berkó Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2014 | World Journal of Gastroenterology2014，20，44： | 3 |
| 2 | Structural and molecular features of intestinal strictures in rats with Crohn's-like disease显示文摘AIM: To develop a new rat model we wanted to gain a better understanding of stricture formation in Crohn's disease(CD).METHODS: Chronic colitis was induced locally by the administration of 2,4,6-trinitrobenzenesulfonic acid(TNBS). The relapsing inflammation characteristic to CD was mimicked by repeated TNBS treatments. Animals were randomly divided into control, once, twice and three times TNBS-treated groups. Control animals received an enema of saline. Tissue samples were taken from the strictured colonic segments and also adjacent proximally and distally to its 60, 90 or 120 d after the last TNBS or saline administrations. The frequency and macroscopic extent of the strictures were measured on digital photographs. The structural features of strictured gut wall were studied by light- and electron microscopy. Inflammation related alterations in TGF-beta 2 and 3, matrix metalloproteinases 9(MMP9) and TIMP1 m RNA and protein expression were determined by quantitative real-time PCR and western blot analysis. The quantitative distribution of caspase 9 was determined by post-embedding immunohistochemistry.RESULTS: Intestinal strictures first appeared 60 d after TNBS treatments and the frequency of them increased up to day 120. From day 90 an intact lamina epithelialis, reversible thickening of lamina muscularis mucosae and irreversible thickening of the muscularis externa were demonstrated in the strictured colonic segments. Nevertheless the morphological signs of apoptosis were frequently seen and excess extracellular matrix deposition was recorded between smooth muscle cells(SMCs). Enhanced caspase 9 expression on day 90 in the SMCs and on day 120 also in myenteric neurons indicated the induction of apoptosis. The m RNA expression profile of TGF-betas after repeated TNBS doses was characteristic to CD, TGF-beta 2, but not TGF-beta 3 was up-regulated. Overexpression of MMP9 and down-regulation of TIMP1 were demonstrated. The progressive increase in the amount of MMP9 protein in the strictures was also obvious between days 90 and 120 but TIMP1 protein was practically undetectable at this time.CONCLUSION: These findings indicate that aligned structural and molecular changes in the gut wall rather than neuronal cell death play the primary role in stricture formation. | Petra Talapka Anikó Berkó Lajos István Nagy Lalitha Chandrakumar Mária Bagyánszki László Géza Puskás éva Fekete Nikolett Bódi | 2016 | World Journal of Gastroenterology2016，22，22： | 3 |
| 3 | A new endoscopic surgical classification and Invasion criteria for pituitary adenomas Involving the cavern- ous sinus显示文摘 | Ceylan S Anik I Koc K | 2011 | Turkish Neurosurgery2011，21，3： | 1 |
| 4 | Endoscopic endonasal transsphenoidal approach for pituitary adenomas invading the cavernous sinus Clinical article 显示文摘 | Ceylan S Koc K Anik I | 2012 | Journal Of Neurosurgery2012，112，1： | 1 |
| 5 | Endoscopic endonasal transsphenoidal approach for pituitary adenomas invading the cavernous sinus显示文摘 | Ceylan S Koc K Anik I | 2010 | J Neurosurg2010，112，： | 1 |
| 6 | A new endoscopic surgical classification and invasion criteria for pituitary adenomas involving the cavernous sinus显示文摘 | Ceylan S Anik I Koc K | 2011 | Turk Neurosurg2011，21，： | 1 |
| 7 | Metallophysical processes in the welding o{ copper and copper alloy welding methods显示文摘 | ANIK S DORN L | 1988 | Welding and Cutting1988，，9： | 1 |
| 8 | a-Glucosidase based bismuth film electrode for inhibitor detection 显示文摘 | Timur S Anik U | 2007 | Anal Chim Acta2007，598，1： | 1 |
| 9 | Al-Si/B4C composite coatings on AI-Si substrate by plasma spray technique显示文摘 | SARIKAYA O ANIK S ASLANLAR S | 2007 | Materials and Design2007，28，9： | 1 |
| 10 | Membranous structures affecting the success of endoscopic third ventriculostomy in adult aqueductus sylvii stenosis 显示文摘 | Anik I Ceylan S Koc K | 2011 | Minim Invasive Neurosurg2011，54，2： | 1 |
| 11 | A1-Si/B4C composite coatings on A1 - Si substrate by plasma spray technique 显示文摘 | Sarikaya O Anik S Aslanlar S | 2007 | Materials and Design2007，28，： | 1 |
| 12 | Development of a microbial biosensor based on carbon nanotube (CNT) modified electrodes显示文摘 | ANIK U ODACI D | 2007 | Electrochemistry Communications2007，9，8： | 1 |
| 13 | Endoscopic endonasal transsphenoidal approach for pituitary adenomas invading the cavernous sinus显示文摘 | Ceylan S Koc K Anik I | | 0，，01： | 1 |
| 14 | A new endoscopic surgical classification and invasion criteria for pituitary adenomas involving the cavernous sinus显示文摘 | Ceylan S Anik I Koc K | 2011 | Turk Neurosurg2011，21，3： | 1 |
| 15 | Extended endoscopic approaches for midline skull-base lesions 显示文摘 | Ceylan S Koc K Anik I | 2009 | Neurosurg Rev2009，32，3： | 1 |
| 16 | Microsurgical anatomy of membranous layers of the pituitary gland and the expression of extracellular matrix collagenous proteins 显示文摘 | Ceylan S Anik I Koc K | 2011 | Acta Neurochir ( Wien)2011，153，12： | 1 |
| 17 | Endoscopic endonasal transsphenoidal approach for pituitary adenomas invading the cavernous sinus显示文摘 | Ceylan S Koc K Anik I | | 0，，01： | 1 |
| 18 | Impact of landfragmentation, farm size, land ownership and crop diversity onprofit and efficiency of irrigated farms in India显示文摘 | Manjunatha A V Anik Asif Reza Speelman S | 2013 | Land Use Policy2013，31，7： | 1 |
| 19 | Wear Behaviour of Plasma-sprayed A1Si/B4 C Composite Coatings 显示文摘 | SARIKAYA O ANIK S CELIK E | 2007 | Materials and Design2007，28，： | 1 |
| 20 | Development of a microbial biosensor based on carbon nanotube (CNT)modified electrodes显示文摘 | Timur S Anik U Odaci D | | 0，，08： | 1 |
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