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| 1 | Translational pancreatic cancer research:a comparative study on patient-derived xenograft models显示文摘AIM To assess the viability of orthotopic and heterotopic patient-derived pancreatic cancer xenografts implanted into nude mice.METHODS This study presents a prospective experimental analytical follow-up of the development of tumours in mice upon implantation of human pancreatic adenocarcinoma samples. Specimens were obtained surgically from patients with a pathological diagnosis of pancreatic adenocarcinoma. Tumour samples from pancreatic cancer patients were transplanted into nude mice in three different locations(intraperitoneal, subcutaneous and pancreatic). Histological analysis(haematoxylin-eosin and Masson's trichrome staining) and immunohistochemical assessment of apoptosis(TUNEL), proliferation(Ki-67), angiogenesis(CD31) and fibrogenesis(α-SMA) were performed. When a tumour xenograft reached the target size, it was reimplanted in a new nude mouse. Three sequential tumour xenograft generations were generated(F1, F2 and F3).RESULTS The overall tumour engraftment rate was 61.1%. The subcutaneous model was most effective in terms of tissue growth(69.9%), followed by intraperitoneal(57.6%) and pancreatic(55%) models. Tumour development was faster in the subcutaneous model(17.7 ± 2.6 wk) compared with the pancreatic(23.1 ± 2.3 wk) and intraperitoneal(25.0 ± 2.7 wk) models(P = 0.064). There was a progressive increase in the tumour engraftment rate over successive generations for all three models(F1 28.1% vs F2 71.4% vs F3 80.9%, P < 0.001). There were no significant differences in tumour xenograft differentiation and cell proliferation between human samples and the three experimental models among the sequential generations of tumour xenografts. However, a progressive decrease in fibrosis, fibrogenesis, tumour vascularisation and apoptosis was observed in the three experimental models compared with the human samples. All three pancreatic patient-derived xenograft models presented similar histological and immunohistochemical characteristics.CONCLUSION In our experience, the faster development andgreatest number of viable xenografts could make the subcutaneous model the best option for experimentation in pancreatic cancer. | Mercedes Rubio-Manzanares Dorado Luis Miguel Marín Gómez Daniel Aparicio Sánchez Sheila Pereira Arenas Juan Manuel Praena-Fernández Juan Jose Borrero Martín Francisco Farfán López Miguel ángel Gómez Bravo Jordi Muntané Relat Javier Padillo Ruiz | 2018 | World Journal of Gastroenterology2018，24，7： | 2 |
| 2 | Flow cytometric characterization of human umbilical cord blood lymphocytes: immunophenotypic features 显示文摘 | D' Arena G Musto P Cascavilla N | 1998 | Haematologica1998，83，3： | 1 |
| 3 | GSK-3beta inhibition/beta-catenin stabilization in ventral midbrain precursors increases differentiation into dopamine neurons显示文摘 | Castelo-Branco G Rawal N Arenas E | | 0，，24： | 1 |
| 4 | Photovoltaic properties of multi-walled carbon nanotubes deposited on n-doped silicon 显示文摘 | Arena A Donato N Saitta G | 2008 | Microelectron J2008，39，12： | 1 |
| 5 | Minimally differentiated acute myeloid leukemia (AML M0): clinico-biological findings of 29 cases显示文摘 | Cascavilla N Melillo L D'Arena G | 2000 | Leuk Lymphoma2000，37，12： | 1 |
| 6 | Phase Ⅲ,randomized,double-blind,placebo-controlled multicenter trial of daily everolimus plus weekly trastuzumab and vinorelbine in trastuzumab-resistant,advanced breast cancer (BOLERO-3)显示文摘 | O' Regan R Ozguroglu M Andre F Toi M Jerusalem G Wilks S Isaacs C Xu B Masuda N Arena FP Yardley D Yap YS Mukhopadhyay P Douma S El-Hashimy M Taran T Sahmoud T Lebwohl D Gianni L | | 0，，15： | 1 |
| 7 | Flow cytometric characterization of human umbilical cord blood lymphocytes: immunophenotypic features显示文摘 | Musto P Cascavilla N | 1998 | Haematologica1998，83，： | 1 |
| 8 | GSK-3β inhibition/β- catenin stabilization in ventral midbrain precursors increases differentiation into dopamine neurons显示文摘 | Castelo-Branco G Rawal N Arenas E | 2004 | J Cell Sci2004，117，24： | 1 |
| 9 | Intracellular trafficking of Brucella abortus in J774 macrophages显示文摘 | Arenas G N Staskevich A S Ahallay A | 2000 | Infect Immun2000，68，7： | 1 |
| 10 | CD117(C-kit) is a restricted antigen of acute myeloid leukemia and characterizes early differentiative levels of M5 FAB subtype显示文摘 | Cascavilla N Musto P D Arena G | 1998 | Haematologica1998，83，5： | 1 |
| 11 | CD117(c-kit) is a restricted antigen of acute myeloid leukemia and characterizes early differentiative levels of M5 FABsubtype显示文摘 | Cascavilla N Musto P D 'Arena G | 1998 | Haematologica1998，83，： | 1 |
| 12 | Capacitive humidity sensors based on MWCNTs/polyelectrolyte interfaces deposited on flexible substrates 显示文摘 | Arena A Donato N Saitta G | 2009 | Microelectronics Journal2009，40，： | 1 |
| 13 | A rootless suture and the loss of the roots of a mountain chain:the Variscan belt of NW Iberia显示文摘 | Martínez Catalán J.R Arenas R Abati J Sánchez Martínez S Díaz García F Fernández Suárez J González Cuadra P Casti(n)eiras P Gómez Barreiro J Díez Montes A González Clavijo E Rubio Pascual F.J Andonaegui P Jeffries T.E Alcock J.E Díez Fernández R López Car | | 0，，： | 1 |
| 14 | Flexible, all-organic ammonia sensor based on dodecylbenzene sulfonic acid-doped polyaniline films显示文摘 | Rizzo G Arena A Donato N | 2010 | Thin Solid Films2010，518，23： | 1 |
| 15 | Flexible ethanol sensors on glossy paper substrates operating at room temperature 显示文摘 | Arena A Donato N Saitta G | 2010 | Sensors & Actuators B : Chemical2010，145，： | 1 |
| 16 | Chronic lymphocytic leukemia-asso- ciated autoimmune hemolytic anemia显示文摘 | D'Arena G Cascavilla N | 2007 | Leuk Lymphoma2007，48，6： | 1 |
| 17 | Wnt- 5a induces Dishevelled phosphorylation and dopaminergic differentiation via a CK1 dependent mechanism 显示文摘 | Bryja V Schulte G Rawal N Grahn A Arenas E | 2007 | J Cell Sci2007，120，4： | 1 |
| 18 | Chronic lymphocytic leukemia-associated autoimmune hemolytic anemia 显示文摘 | D'ARENA G CASCAVILLA N | 2007 | Leuk Lymphoma2007，48，： | 1 |
| 19 | Alkaline and alkaline-earth metals compounds as catalysts for the methanolysis of sunflower oil 显示文摘 | ARZAMENDI G ARGUI N ARENA E CAMPO I | 2008 | Catalysis Today2008，，133135： | 1 |
| 20 | Adult and childhood acute lymphblastic leukmia:clinico-biological differences based on CD34 an an antigen expression显示文摘 | Cascavilla N Musto P D Arena G | 1997 | Haematologica1997，82，1： | 1 |
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