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| 1 | CYP24A1 inhibition facilitates the anti-tumor effect of vitamin D3 on colorectal cancer cells显示文摘AIM:The effects of vitamin D3 have been investigated on various tumors, including colorectal cancer (CRC). 25-hydroxyvitamin-D3-24-hydroxylase (CYP24A1), the enzyme that inactivates the active vitamin D3 metabolite 1,25-dihydroxyvitamin D3 (1,25-D3), is considered to be the main enzyme determining the biological halflife of 1,25-D3. During colorectal carcinogenesis, the expression and concentration of CYP24A1 increases significantly, suggesting that this phenomenon could be responsible for the proposed efficacy of 1,25-D3 in the treatment of CRC. The aim of this study was to investigate the anti-tumor effects of vitamin D3 on the human CRC cell line Caco-2 after inhibition of the cytochrome P450 component of CYP24A1 activity. METHODS:We examined the expression of CYP24A1 mRNA and the effects of 1,25-D3 on the cell line Caco-2 after inhibition of CYP24A1. Cell viability and proliferation were determined by means of sulforhodamine-B staining and bromodeoxyuridine incorporation, respectively, while cytotoxicity was estimated via the lactate dehydrogenase content of the cell culture supernatant. CYP24A1 expression was measured by realtime reverse transcription polymerase chain reaction. A number of tetralone compounds were synthesized to investigate their CP24A1 inhibitory activity. RESULTS:In response to 1,25-D3, CYP24A1 mRNA expression was enhanced significantly, in a time- and dose-dependent manner. Caco-2 cell viability and proliferation were not influenced by the administration of 1,25-D3 alone, but were markedly reduced by coadministration of 1,25-D3 and KD-35, a CYP24A1-inhibiting tetralone. Our data suggest that the mechanism of action of co-administered KD-35 and 1,25-D3 does not involve a direct cytotoxic effect, but rather the inhibition of cell proliferation. CONCLUSION:These findings demonstrate that the selective inhibition of CYP24A1 by compounds such as KD-35 may be a new approach for enhancement of the anti-tumor effect of 1,25-D3 on CRC. | János P Kósa Péter Horváth János Wlfling Dóra Kovács Bernadett Balla Péter Mátyus Evelin Horváth Gábor Speer István Takács Zsolt Nagy Henrik Horváth Péter Lakatos | 2013 | World Journal of Gastroenterology2013，19，17： | 5 |
| 2 | Diagnosis of goose circovirus infection in Hungarian geese samples using poly- merase chain reaction and dot blot hybridization tests 显示文摘 | Balla N W Smyth J A Weston J H | 2004 | Avian Pathol2004，33，1： | 1 |
| 3 | Multiple sclerosis and autoimmune diseases 显示文摘 | BAKER H W BALLA J I MACKAY I R | 1972 | Aus N Z J Med1972，2，： | 1 |
| 4 | Is more ever too much:the number of indicators per factor in confirmatory factor analysis显示文摘 | Marsh H W Han K T Balla J R Grayson | 1998 | Multivariate Behavioral Research1998，33，2： | 1 |
| 5 | Once-monthly subcutaneous CERA maintains stable hemoglobin control in patients with chronic kidney disease on dialysis and converted directly from epoetin one to three times weekly 显示文摘 | Sulowicz W Locatelli F Ryckelynck JP Balla J Csiky B Harris K | 2007 | Clin J Am Soc Nephrol2007，2，4： | 1 |
| 6 | Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA显示文摘 | Ballas Z K Rasmussen W L Krieg A M | 1996 | J Immunol1996，157，5： | 1 |
| 7 | Hemin: a possible physiological mediator of low density lipoprotein oxidation and endothelial injury显示文摘 | BALLA G JACOB H S EATON J W | 1991 | Arterioscler Thromb1991，11，6： | 1 |
| 8 | Goodness-of-fit indexes in confirmatory factor analysis: The effect of sample size显示文摘 | Marsh H W BALLA J R MCDONALD R P | 1988 | Psychological Bulletin1988，103，： | 1 |
| 9 | Induction of NK activity in murine and human cells by CpG motifys in oligodoxynucleotides and bacterial DNA显示文摘 | Ballas Z K Rasmussen W L Krieg A M | 1996 | J Immunol1996，157，5： | 1 |
| 10 | Goodness-of-fit indexes in confirmatory factor analysis:The effect of sample size显示文摘 | Marsh H W Balla J R McDonald R P | 1988 | Psychological Bulletin1988，103，3： | 1 |
| 11 | Is more ever too much The number of indicators per factor in confirmatory factor analysis 显示文摘 | Marsh H W Har K T Balla J R Grayson D | 1998 | Multivarial Behavioral Research1998，33，2： | 1 |
| 12 | Diagnosis of goose circovirus infection in Hungarian geese samples using polymerase chain reaction and dot blot hybridization tests 显示文摘 | Balla N W Smyth J A | 2004 | Avian Pathology2004，33，1： | 1 |
| 13 | merican society ofhospital pharmacists ASHP technical assistance bulletin onhandling cytotoxic and hazardous drugs显示文摘 | Krieg A M Ballas Z K Rasmussen W L | 1990 | Am JhospPharm1990，47，8： | 1 |
| 14 | Electrocoagulation/electroflotation of reactive,disperse and mixture dyes in an externalloop airlift reactor显示文摘 | BALLA W ESSADKI A H GOURICH B | | 0，，1： | 1 |
| 15 | Goodness-of-fit indices in confirmatory factor analysis: The effect of sample size and model complexity 显示文摘 | Marsh H W Balla J R | 1994 | Quality & Quantity1994，28，： | 1 |
| 16 | Electrocoagu- lation/electroflotatian of reactive, disperse and mixture dyes in an external - loop airlift reactor显示文摘 | BALLA W ESSADKI A H GOURICH B | 2010 | Journal of Hazardous Ma- terials2010，184，13： | 1 |
| 17 | Engineered por- ous metals for implants显示文摘 | Balla VK Xue W Bose S | 2008 | JOM2008，60，5： | 1 |
| 18 | Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA显示文摘 | Ballas Z D Rasmussen W L Krieg A M | 1996 | J Immunol1996，157，： | 1 |
| 19 | Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA显示文摘 | Ballas Z K Rasmussen W L Krieg A M | 1996 | J Immunol1996，157，5： | 1 |
| 20 | Induction of NK activity in murine and human cells by CpG motifs in oligodeoxynucleotides and bacterial DNA显示文摘 | Ballas Z D Rasmussen W L Krieg A M | 1996 | J Immunol1996，157，： | 1 |
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